Fars2 (NM_001013139) Rat Tagged ORF Clone

CAT#: RR214056

Fars2 (Myc-DDK-tagged ORF) - Rat phenylalanyl-tRNA synthetase 2, mitochondrial (Fars2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR214056

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fars2
• Synonyms: Fars1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214056 representing NM_001013139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGTTTGGCCTTCATCAGAGCTGCCCATGAGCACCTCTACCTGGTGAGGAAAGTCAGTCATGTCT
  GCAGATGTCATCAACATCGGGCTTGGGGCTCAAGGCCAGCTGCATCGCAGTTTGCTGTCCAAGGTGCTCC
  AGGCCGTGTGCTGGAGCTGCTTGGTAAATCCTACCCTCAAGATGACCACACTAACCTCACCCAGAAAGTC
  CTTTCCAAGGTGGGCAGGAACCTGCACAACCAGAAGTTCCACCCTCTGTGGCTGATTAAGGAGCGGGTGA
  AGGAGCACTTCTACCAGCAGTACATGGGGCGATCCAGGACACCTCTGTTCTCCGTCTATGACCAGCTTTC
  CCCAGTGGTCACCACCTGGCAGAACTTCGATAGCCTGCTAATCCCAGCTGACCACCCCAGCAGGAAGAAG
  GGGGACAACTATTACTTGAATCGGGGACACATGCTGAGAGCACACACATCAGCACATCAGTGGGACTTGC
  TGCATGCGGGGCTCAATGCCTTCCTCGTGGTAGGTGATGTGTATCGGCGTGACCAGATTGATTCCCAGCA
  CTACCCAGTTTTCCACCAGCTGGAGGGCGTCCGACTCTTCTCCAAGCATGAGTTATTTGCTGGTGTAAAG
  GATGGAGAAAGCTTGCAGCTCTTTGAAGAAAGTTCTCGTTCTGCTCATAAGCAAGAGACACACACCATGG
  AGGCTGTGAAGCTTGTGGAATTTGACCTTAAACAAGTACTCACTAGGCTTGTGACACATCTTTTTGGAGA
  TGGGCTGGAGGTCCGGTGGGTGGACTGCTACTTTCCCTTTACCCACCCTTCCTTCGAGATGGAGATCAAC
  TTCCGTGGAGAATGGCTTGAAGTCCTCGGCTGCGGGGTGATGGAACAACAGCTTGTCAATTCAGCTGGTG
  CTCAGGATCGGATCGGCTGGGCTTTTGGCCTGGGATTAGAGAGACTGGCTATGGTCCTCTACGACATCCC
  GGACATCCGCCTTTTCTGGAGTGAGGATGAGCGCTTCCTGAAGCAGTTCCGCCTATCAGACATCAATCAA
  AGCGTCAAGTTTCAGAGATGGTTCTTCCAAGAAGAAAGGGCCACAGGAATACAAAGGAAGATGGGAAGAC
  AGCTCTGTCCTTTTAGCAAGTACCCCGCTGTGTTCAATGACATTTCATTCTGGCTGCCCTCCGAGAACTA
  CACGGAGAATGATTTCTATGACATTGTCCGAACAGTCGGAGGAGACCTGGTGGAAAAGGTTGATCTTATA
  GACAAGTTTGAACATCCAAAGACACACAGAACCAGCCATTGCTACCGCATTACCTACCGGCACATGGAAC
  GGACACTGTCCCAGAGAGAAGTCAGCAGTGTCCACCAGGCTGTGCAGGAGGCCGCTGTGCAGCTACTGGG
  TGTGGAAGGCCGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214056 representing NM_001013139
  Red=Cloning site Green=Tags(s)
  
  MVCLAFIRAAHEHLYLVRKVSHVCRCHQHRAWGSRPAASQFAVQGAPGRVLELLGKSYPQDDHTNLTQKV
  LSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMGRSRTPLFSVYDQLSPVVTTWQNFDSLLIPADHPSRKK
  GDNYYLNRGHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQIDSQHYPVFHQLEGVRLFSKHELFAGVK
  DGESLQLFEESSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEIN
  FRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQFRLSDINQ
  SVKFQRWFFQEERATGIQRKMGRQLCPFSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLI
  DKFEHPKTHRTSHCYRITYRHMERTLSQREVSSVHQAVQEAAVQLLGVEGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013139
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013139.1, NP_001013157.1
• RefSeq Size: 1782 bp
• RefSeq ORF: 1419 bp
• Locus ID: 306879
• UniProt ID: Q6AYQ3
• Cytogenetics: 17p12
• MW: 55.2 kDa
• Gene Summary: Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.[UniProtKB/Swiss-Prot Function]