Afp (NM_012493) Rat Tagged ORF Clone

CAT#: RR213708

Afp (Myc-DDK-tagged ORF) - Rat alpha-fetoprotein (Afp), (10 ug)

Product Images

Circular map for RR213708

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Afp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213708 representing NM_012493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGCCAGCAACCATGAAGTGGAGCGCATCCATTTCCTTCCTTCTCCTGCTAAATTTTGCTGAAC
  CCAGAGTACTGCACACAAATGAGTTTGGAATAGAATCCACATTAGACTCTTCCCAGTGCCCGACAGAGAA
  AAATATGTTTAACGTAGCTACCATTGTCGTTGCCCAGTTTGTTCAGGATGCCACAAAGGCCGAAGTAAAC
  AAAATGAGTAGCGATGCGTTGGCTGCAATGAAGGAAAACACTGGCGATGGGTGTTTAGAAAACCAGCTAT
  CTGTGTTTCTGGATGAAATTTGCCACGAGACGGAACTCTCTAACAAGTATGGATTCTCAGGTTGCTGCAA
  CCAAAGCGGGGTGGAAAGACACCAGTGTCTGCTGGCTCGCAAGAAGACCGCTCCAGACTCTGTCCCACCC
  TTCCACTTTCCAGAAACTGCCGAGAGTTGCCCAGCATATGAAGAAAACAGGGCGATGTCCATAAACACGT
  TCATCTACGATGTGTCGAAGAGGAACCCCTTCCTGTATGCTCCCACCATTCTTTACTTAGCCGCTCAGTA
  TGACAAGGCAGTTCCAGCATGCTGCAAAGCTGACAACATGGAGGAATGCTTCCAGACAAAGAGAGCATCA
  ATGGCAAAGGAATTAAGAGAAGGAAGCATGCTCAATGAGCATGTGTGTGCAGTAATAAGAAAGTTTGGAT
  CCCGGAACCTCCAGGCTGTACTCATCATTAAACTAAGTCAAAAGTTTCCCAAAGCAAATATTACTGAGAT
  TCGGAAGCTGGCCCTGGACGTGGCCCACATCCACGAGCAGTGCTGCCACGGCAACGCGATGGAGTGCCTA
  CAGGATGGGGAAAGCGTCATGACACATATATGTTCTCAACAAGAAATTCTGTCGAGCAAAACAGCAGAGT
  GCTGCAAACTACCCACCATCGAGCTCGGCTATTGCATAATTCATGCCGAAAATGGCGACAAACCGGAAGG
  TTTAACTCTGAACCCAAGCGAGTTTTTGGGAGACAGAAATTTTGCCCAGTTTTCTTCAGAGGAAAAACTC
  CTGTTTATGGCAAGCTTTCTTCATGAATATTCAAGAAATCACCCCAACCTTCCTGTCTCAGTCATTCTAA
  AAACTGCTAAGTCATACCAGGAAATTTTGGAGAAGTGCTCCCAGTCTGAAACGCCATCGAAATGCCAGGA
  CAATATGGAAGAAGAATTGCAAAAACACATCCAGGAGAGCCAGGCACTGGCCAAACAAAGTTGTGATCTC
  TACCAGAAGTTAGGACCCTACTACTTACAAAATCTGTTCCTCATTGGCTACACGCGGAAGGCTCCTCAGC
  TGACCTCAGCAGAGCTGATAGACCTGACAGGGAAGATGGTGAGCATTGCCTCAATGTGCTGCCAGCTCAG
  TGAGGAGAAACGGTCCGCCTGCGGTGAGGGACTGGCCGACATTTACATTGGACACTTGTGTTTAAGACAT
  GAGGCAAACCCTGTGAACTCCGGTATCAACCACTGCTGCAGTTCCTCGTATTCCAACAGGAGGCTCTGCA
  TCACCAGCTTTCTGAGGGACGAAACCTACGTCCCTCCACCATTCTCTGAAGACAAATTCATCTTCCACAA
  GGATCTGTGCCAAGCTCAGGGCCGAGCCCTACAGACCATGAAACAAGAGCTTCTCATTAACCTGGTGAAA
  CAAAAGCCTGAAATGACAGAGGAGCAGCACGCGGCTGTCACTGCTGATTTCTCTGGCCTCTTGGAGAAGT
  GCTGCAAAGACCAGGATCAGGAAGCCTGTTTCGCAAAAGAGGGTCCAAAGTTGATTTCCAAAACTCGTGA
  GGCTTTGGGGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213708 representing NM_012493
  Red=Cloning site Green=Tags(s)
  
  MKQPATMKWSASISFLLLLNFAEPRVLHTNEFGIESTLDSSQCPTEKNMFNVATIVVAQFVQDATKAEVN
  KMSSDALAAMKENTGDGCLENQLSVFLDEICHETELSNKYGFSGCCNQSGVERHQCLLARKKTAPDSVPP
  FHFPETAESCPAYEENRAMSINTFIYDVSKRNPFLYAPTILYLAAQYDKAVPACCKADNMEECFQTKRAS
  MAKELREGSMLNEHVCAVIRKFGSRNLQAVLIIKLSQKFPKANITEIRKLALDVAHIHEQCCHGNAMECL
  QDGESVMTHICSQQEILSSKTAECCKLPTIELGYCIIHAENGDKPEGLTLNPSEFLGDRNFAQFSSEEKL
  LFMASFLHEYSRNHPNLPVSVILKTAKSYQEILEKCSQSETPSKCQDNMEEELQKHIQESQALAKQSCDL
  YQKLGPYYLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASMCCQLSEEKRSACGEGLADIYIGHLCLRH
  EANPVNSGINHCCSSSYSNRRLCITSFLRDETYVPPPFSEDKFIFHKDLCQAQGRALQTMKQELLINLVK
  QKPEMTEEQHAAVTADFSGLLEKCCKDQDQEACFAKEGPKLISKTREALGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012493
• ORF Size: 1833 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012493.2, NP_036625.2
• RefSeq Size: 1986 bp
• RefSeq ORF: 1836 bp
• Locus ID: 24177
• Cytogenetics: 14p22
• MW: 68.4 kDa
• Gene Summary: plasma protein expressed in the fetal liver and yolk sac [RGD, Feb 2006]