Adam30 (NM_001109600) Rat Tagged ORF Clone

CAT#: RR213699

Adam30 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase domain 30 (Adam30), (10 ug)

Product Images

Circular map for RR213699

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213699 representing NM_001109600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCAGTGTGGGCCTCCCTCCCCCAACACCGCTGGCTGCTTCTGGCGATGCTCTTTCTTGAGGCTA
  TGGCCGAAGACCTACTATTTGACCCTGACTGGGGCTTCGACTCGTATGAAATCACCATTCCCAAAGTACT
  AAGCTTGAGGAAAGAGGCGCAGGGTGTCGACAGTTCTTTGTCATACCTCTTACAGATAGAAGGCAAGGAG
  CGTGTGGTGCACTTGCGGCCCAAGAAGTTACTGCTGCCCAGACACCTGCCAGTTTTCTCCTTCACCCAAG
  AGGACAGTCTGATAGAGGACTACCCACATATTCCAGACCAGTGTAATTACGTGGGCTTCGTGGAGGGCTC
  TCAGGAAGCCGAATCTACTCTGAGTACATGCATGGGGGGTCTGCGAGGCGTCTTGAACATTGACTCCAGA
  TACTACCAAATCGAACCCCTCAGGGCCTCTTCCCGTTTTGAACATGTAGTGTATCTCCTAACTGAGGACA
  ACTTCGGCAATGAGACCTGTGGTGTGGTTGATGAAGAAACAGATGGGCAAACAGCCGAGGAAGAGGAAGT
  GGCTAGAATAAAAAACTTTCATCAGTCCTACAAGCACCAAAGGTACTTGGAATTGGTCATGGTCTTTGAC
  CTGAGTAGATATGTGTTTACCCGAGGCAATATGACTAGAATGGTAGAGGACGCCATTCTCTTGGCTTCAA
  TTGCAGACACCTACTTTCAGGATGTCAGCTTGAAGATTCAACTGAAGGCGCTTGAAGTCTGGACGCAGTT
  TGACAAAATACATCTTTATTATTCCTCTTTATCCCAAGTGTTAGGCCAATTTGTGCTTTACAAAAGAGCT
  TCGCTGCATCACCGGCTTCCTGCGGACTGGGCACATTTATACATTGCAAGGAGATACAGTGATGCTACTG
  CGTGGTCTTGGGGAAGGGCATGTGAAGAATTCCATGCTGGATCCGCAAGCAGCTTCCTCAACGTTAATCT
  CCTGGGACCTGCCACTTGGACTGCTCACGAAATGGGCCATTGTGTGGGGATGTATCATGATGAGGAATTC
  TGTCAGTGCAGAGGCAGGAAGAGCTGTGTCATGGGTACAGGACGTACAGGGTTTAGTAACTGTAGTTACC
  ATCAGTATTTTTTACACGCGTCTTACAAAATGTCCTATTGTCTATCTGACTTCCCAAAGCTGCATATAGT
  AGAGAGGTGTGGAAATAAAATTGTGGAAAACAAGGAGGAGTGTGACTGCGGCTCCGAAGAAGAGTGCAAA
  AGCGACCTGTGCTGCGGGCCAGACTGTAAGTGGAAAGAGGGCGTCAACTGCTCCTCCGGGCTCTGTTGTC
  ATAAGTGCAACTTTCTTCCATCGGGCTATGTGTGCAGGGAGGAAGAAAATGAATGCGACCTCGCAGAGTA
  CTGTGACGGGCTCTCAGGTTTCTGTCCGAAAGACTATTACAAGCAGGATGGGACTCCGTGCAGGTATGGA
  GGCTTTTGCTTCCATAAGGGGTGCAGATCCAGGTACTTGCAGTGCCAGGCCATTTTTGGACGTAGAGCCA
  AGGAGGCCCCTCGCCAGTGTTACGATGCAGTTAACATGATAGGTGACCAGTCTGGGAACTGTGGCATTGT
  AAATGCTACCCGATACACCAAGTGTCATAGAGACAACACGATCTGTGGTCGGCTCCAGTGTGTAAACATT
  AAAGCCCTCTCCAGATTTCCTGATCACACCCTTCTACTGAGCACCCACCTGAAGAAAGACAATCTCAAGT
  GCTGGGGCACGAGCTACCATGGGACCATGATACCGCACGGGGTACCCGACATAGGTGATGTCAACGACGG
  TACCTCCTGCGGTCCTAACCGGGTGTGCCTTAACAGGACTTGCGTTGACTACGCCACCCTGAAGTTCGAC
  TGTTTGCCTAAAAAGTGCAATAGCCGGGGTGTTTGCAACAACAAAAAGAACTGCCATTGCACATATGGCT
  GGGAACCCCCATTCTGTGTGGAGGTAGGGTACGGTGGGAGCATTGACAGTGGGCCCCCTGGACCCAGGGC
  GAGACTCGCAAAGGTTCACGTGCTGTACATGCTGCGTACTATTCTGTTCATCTTTTCTGTGATCGTTGTG
  TGTTTTACGCAGCGAATGAAAAATTTGTTTCGTAAGCGCCAGCAAAAAAACCCAGCTGAGACTCAGGGGA
  AGAAGAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213699 representing NM_001109600
  Red=Cloning site Green=Tags(s)
  
  MRSVWASLPQHRWLLLAMLFLEAMAEDLLFDPDWGFDSYEITIPKVLSLRKEAQGVDSSLSYLLQIEGKE
  RVVHLRPKKLLLPRHLPVFSFTQEDSLIEDYPHIPDQCNYVGFVEGSQEAESTLSTCMGGLRGVLNIDSR
  YYQIEPLRASSRFEHVVYLLTEDNFGNETCGVVDEETDGQTAEEEEVARIKNFHQSYKHQRYLELVMVFD
  LSRYVFTRGNMTRMVEDAILLASIADTYFQDVSLKIQLKALEVWTQFDKIHLYYSSLSQVLGQFVLYKRA
  SLHHRLPADWAHLYIARRYSDATAWSWGRACEEFHAGSASSFLNVNLLGPATWTAHEMGHCVGMYHDEEF
  CQCRGRKSCVMGTGRTGFSNCSYHQYFLHASYKMSYCLSDFPKLHIVERCGNKIVENKEECDCGSEEECK
  SDLCCGPDCKWKEGVNCSSGLCCHKCNFLPSGYVCREEENECDLAEYCDGLSGFCPKDYYKQDGTPCRYG
  GFCFHKGCRSRYLQCQAIFGRRAKEAPRQCYDAVNMIGDQSGNCGIVNATRYTKCHRDNTICGRLQCVNI
  KALSRFPDHTLLLSTHLKKDNLKCWGTSYHGTMIPHGVPDIGDVNDGTSCGPNRVCLNRTCVDYATLKFD
  CLPKKCNSRGVCNNKKNCHCTYGWEPPFCVEVGYGGSIDSGPPGPRARLAKVHVLYMLRTILFIFSVIVV
  CFTQRMKNLFRKRQQKNPAETQGKKR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109600
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109600.1, NP_001103070.1
• RefSeq Size: 3441 bp
• RefSeq ORF: 2181 bp
• Locus ID: 690547
• Cytogenetics: 2q34
• MW: 82.8 kDa