Radil (NM_001037218) Rat Tagged ORF Clone

CAT#: RR213686

Radil (Myc-DDK-tagged ORF) - Rat Rap GTPase interactor (Radil), (10 ug)

Product Images

Circular map for RR213686

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Radil
• Synonyms: RGD1306155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213686 representing NM_001037218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTATGGGACTCAGTTCATCATGTCTCCTCCCACCAAGAATAAACTGAAGCGACAGAGCCAGCTAC
  TATCTTCTATGCTGTCCCGGACACTGAGCTACAAGTATCGTGACCTAGACTCCACCTTCTGCAGCCTAGG
  TGCCAGTGATGACCCCTCGGAGCTCTCCACGCAGCTCTCAGCCCCTGGGGTCCTGAAGGTGTTTGGGGAC
  AGTGTCTGTACAGGCACCCACTATAAGAGCGTCCTGGCCACTGGCGCTTCCAGCGCCCAGGAGCTGGTGA
  AAGAGGCACTGGAGCGCTATGCCCTGGATCCCGAGTGTGCCAGCCAGTATGTGCTATGTGATGTTGTGGG
  CCAGGCTGGGGACTCTGGGCAGCGGTGGCAGGCCCAGTGCTTCCGAGTGTTTGGGGACAATGAGAAGCCC
  CTCTTGATTCAGGAGTTATGGAAGCCCCGAGAAGGCTTGTCCCGGAGGTTTGAGCTAAGGAAGAAGTCAG
  ATGTGGAAGAGCTGGCGGCGAGGGATGTGGATACCACCACAGCAGGGATAAACGCCCAAGCTCGGAGGCT
  ACAGCGTATCCGTGCCAAAGGAACCCCAGCCCTCACCTCTGAGACTGCCCAAAGCTCCCCACCAACCGGG
  CTGCGCCGTACAGTCAGCGAGACCAGCCTAAGCCCAACACCAACGCTACCTGAGGCTGCCCAGAGGCCTG
  AGGAGCCCCTCCCTGACAGCATGCGTTACTCACTGTACCAGTGCCCGCACCTGCTGCTGTTGCAGGGCTA
  CAACCAGCAGCACGACAGCCTGGTGTACGTGCTCAGCCGGGAGCGGCACACAGTGGGCCAGCGCACGCCC
  TCCAGCAAACCCAGCATCAGCTTGTCTGCCCCAGACATCCTGCCTCTGCACTGCACCATTCGCCGCCACC
  AGTCCCCAGATGGTGGCCCGGCGGGGACCAGGCTAGTGCTGGAGCCCATCGCAGGGGCACCGGTGTCAGT
  CAACTTCTCAGAAGTGGGAAGGAACCCCGTGGTGCTACAACATGGCGACCTGCTCTCCCTGGGTCTCTAC
  TACCTGCTGCTGTTCAAGGACCCAGGGCAGGCGCAGCCACTGCCTGCCTGTGCCCTCGCCCGTCTTGGGG
  CAGCACCCCAGAGCTGTAGAATGTGTGGTGCGGCGCTCAAAGCCCGCGGGCCGGCTTCCTTGCCTGCTGC
  TGTGGTGCGGCGGCGGTCACTGTTCCTGGAGTTTGAGCCCGACGTGGAGGACACACTGCTGCAGAGGATC
  ATGACACTGATTGAGCCTGGAGGGGATGACCACAAGCTGACACCCGCCTTCCTCCTCTGCCTCTGCATCC
  AGCACTCTGCCACTCACTTCCAGCCGGGTACCTTCAGGCATCTCCTGCTCAAGATCGCCAAAAGGATCCG
  AGACACTGTCTGGGAGAAGACCAAAGAACTAGCAGAAAAGCAGGCGCAACTGCAGGAACCCATCTCCTGG
  GCCAGCTTCCCCATGGCCGACCTGGTTCCTGACCTGCAGCACATTCTCTTTTGGATGTCAAACTGCATCG
  AACTCCTGTACTTCATCCAACAGAAGTCGCCACTGTACGTGCAGAGCATGGAGGAGGAGCTGGATGTCAC
  AGGCTCCAAGGAGTCGCTGTTCTCCTGCACGCTGACAGCCAGTGAGGAGGCCATGGCGGCCCTGGAGGAG
  GTGGTGCTGTACGCTTTCCAGCAGTGTGTGTATTACCTCTCCAAGTGCCTGTATGTCTGCCTCCCGGCCC
  TCTTGGAATGCCCCCCATTTCAGACAGAGCGCAGGGAGAGCTGGCGCTCGGGCCCCGCTCTGCCTGAAGA
  GCTTCGGCGCGTGGTGTCGGTGTTCCAGGCCACCATGGACCTCCTGCAGCAGCTGCAGATGCATCCTGAG
  GTGGCCTCTCAGATGCTCGCTTACCTCTTCTTCTTCTCTGGTACACTGCTCCTCAATCAGGTGCTGGACA
  AGGGGCCCTCCCTGAGCTGCTTCCACTGGCCCAGGGGTGTCCAGGTGTGTGCCCGCCTGCAGCAGTTTCT
  GGAGTGGGCCCGCAGCGCTGGCCTCGGAGCACCTGCCGAGCGCTTCTTTCGGAAGCTTTCCTGCACCCTG
  CATCTGCTGGCCACTCCCCGTGCTCAGCTTGTCCAGATGAGCTGGGCCACCCTACGAGCCACCTTCCCTG
  CCCTGAACCCTGCTCAGCTGCACCGGCTGCTGACTCAGTACCAACTGGCCTCTGCCATGGGCCCTGTGAG
  CGCTTGGGATCCAGGAGCCCAAGAGAGCCCTGAGGCCTTCCAGACAGAGGACATCCTGGAGTCCTATGAG
  AACCCGCCACCCATCGTTCTGCCGAGCCAGGGCTTCCAGGTGGACCTGGAGGCCGACTGCCCGGAGGACA
  GCATCTACCAGCACCTGCTCTACATCCGCCACTTCCTGTGGGGACTGCGGGGCCAAGCAAGCCCTGAGAG
  TGGGCCTGCCCAGCCTGACAGCATCGAGGGCCTGTACCACACAATTCCTGAGGGGCATCTGGAAGGCCAT
  GGCTGCCCCTTGACTAATAGAGAACAAGGCAGAGGAGCTGTGGAAACTGCTCCTGGCCATTCTCTTCCTG
  TGCCTGGAGCTCCCCGGGCACAGGGTCCCCCTGGAAGACAGCCTCCCCGAGGGGACCGCAGGGGCTCCCA
  GGCTGGCTCTCTGCACACTGACTCCTCCTGCCTGCTCACTCCTCCCAGCACACCACTGGGCCTGGAGCCT
  GGAGGCCCCACCTGGCCAGAGCCCAGCGGCCTCTGTGGAAAAGCGCTCCTCGAAGGGCAGAGGAATGGAC
  CAGGTGGTCTTCCTGGTGCAGTTCTAGAAGGAGACGCGGTTCAGGCTGCGGCTGAGCCTCCTGCCGAGGC
  CTCCAGCCCCAGCTCCAGCACAGAGGACTTCTGCTACGTGTTCATGGTGGAGCTGGAGCGAGGCCCCTCG
  GGGCTGGGGATGGGCCTGATCGATGGCATGCACACACCTCTGGAGGCCCCGGGACTCTACATACAGACCT
  TGCTTCCTGGGAGCCCCGCGGCATCTGATGGACGGCTGTCCCTGGGAGACCAAATCCTGGAGGTGAACGG
  CAGCAGCCTGATGGGTGTCAGCTACATGAGGGCAGTGGATCTCATCCGACATGGAGGCAAGAAGATGCGA
  TTTCTGGTTGCCAAGTCTGACATGGAAACGGCCAAGAAAATCCGCTTCCGCAAACCCCCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213686 representing NM_001037218
  Red=Cloning site Green=Tags(s)
  
  MFYGTQFIMSPPTKNKLKRQSQLLSSMLSRTLSYKYRDLDSTFCSLGASDDPSELSTQLSAPGVLKVFGD
  SVCTGTHYKSVLATGASSAQELVKEALERYALDPECASQYVLCDVVGQAGDSGQRWQAQCFRVFGDNEKP
  LLIQELWKPREGLSRRFELRKKSDVEELAARDVDTTTAGINAQARRLQRIRAKGTPALTSETAQSSPPTG
  LRRTVSETSLSPTPTLPEAAQRPEEPLPDSMRYSLYQCPHLLLLQGYNQQHDSLVYVLSRERHTVGQRTP
  SSKPSISLSAPDILPLHCTIRRHQSPDGGPAGTRLVLEPIAGAPVSVNFSEVGRNPVVLQHGDLLSLGLY
  YLLLFKDPGQAQPLPACALARLGAAPQSCRMCGAALKARGPASLPAAVVRRRSLFLEFEPDVEDTLLQRI
  MTLIEPGGDDHKLTPAFLLCLCIQHSATHFQPGTFRHLLLKIAKRIRDTVWEKTKELAEKQAQLQEPISW
  ASFPMADLVPDLQHILFWMSNCIELLYFIQQKSPLYVQSMEEELDVTGSKESLFSCTLTASEEAMAALEE
  VVLYAFQQCVYYLSKCLYVCLPALLECPPFQTERRESWRSGPALPEELRRVVSVFQATMDLLQQLQMHPE
  VASQMLAYLFFFSGTLLLNQVLDKGPSLSCFHWPRGVQVCARLQQFLEWARSAGLGAPAERFFRKLSCTL
  HLLATPRAQLVQMSWATLRATFPALNPAQLHRLLTQYQLASAMGPVSAWDPGAQESPEAFQTEDILESYE
  NPPPIVLPSQGFQVDLEADCPEDSIYQHLLYIRHFLWGLRGQASPESGPAQPDSIEGLYHTIPEGHLEGH
  GCPLTNREQGRGAVETAPGHSLPVPGAPRAQGPPGRQPPRGDRRGSQAGSLHTDSSCLLTPPSTPLGLEP
  GGPTWPEPSGLCGKALLEGQRNGPGGLPGAVLEGDAVQAAAEPPAEASSPSSSTEDFCYVFMVELERGPS
  GLGMGLIDGMHTPLEAPGLYIQTLLPGSPAASDGRLSLGDQILEVNGSSLMGVSYMRAVDLIRHGGKKMR
  FLVAKSDMETAKKIRFRKPPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037218
• ORF Size: 3213 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037218.2, NP_001032295.2
• RefSeq Size: 3687 bp
• RefSeq ORF: 3216 bp
• Locus ID: 304299
• Cytogenetics: 12p11
• MW: 117.3 kDa