Ddr1 (NM_013137) Rat Tagged ORF Clone

CAT#: RR213471

Ddr1 (Myc-DDK-tagged ORF) - Rat discoidin domain receptor tyrosine kinase 1 (Ddr1), (10 ug)

Product Images

Circular map for RR213471

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddr1
• Synonyms: Cak; Drd1; PTK3D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213471 representing NM_013137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGGGACCCTCTCATCTCTCCTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA
  AGGGACATTTTGACCCAGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCAGACAGCGA
  TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCCGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA
  GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACCTGCAGGTGGACCTTCGTA
  GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG
  AAGCTACCGGTTGCGTTACTCCCGAGATGGCCGACGATGGATGGACTGGAAAGACAGATGGGGTCAGGAG
  GTGATTTCGGGTAACGAGGATCCTGGGGGAGTGGTGCTGAAGGATCTCGGGCCCCCCATGGTGGCCCGGC
  TGGTCCGTTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT
  CTGGCGGGACGGACTCTTGTCCTATACAGCCCCCGTGGGGCAGACCATGCAATTATCTGAGATGGTGTAT
  CTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTACGGTGGTCTGGGCCAACTGGCAG
  ACGGCGTGGTGGGGCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGTTTGGCCAGGCTATGACTATGT
  GGGATGGAGCAACCATAGCTTCCCCAGCGGCTACGTGGAGATGGAGTTTGAGTTTGACCGGCTGAGGTCT
  TTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTCCCAGGCGGGGTGGAAT
  GCCGGTTTAAAAGGGGTCCGGCCATGGCCTGGGAAGGAGAGCCTGTACGCCATGCCCTGGGGGGTAGCCT
  TGGAGACCCCAGAGCCAGGGCCATCTCGGTGCCCCTGGGTGGTCACGTGGGCCGCTTCCTGCAGTGCAGA
  TTCCTCTTTGCAGGACCCTGGTTACTCTTCAGTGAGATCTCTTTCATCTCGGATGTGGTGAACGACTCGT
  CTGACACCTTCCCACCAGCCCCCTGGTGGCCGCCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGAGCT
  GGAGCCCCGGGGTCAACAGCCTGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGCCTG
  GTGGCCATCATCCTTCTCCTCCTCCTTATCATTGCCCTGATGCTCTGGAGGCTGCACTGGCGCCGGCTGC
  TCAGCAAGGCAGAGCGCCGGGTGTTGGAGGAGGAGCTGACGGTTCACCTCTCTGTCCCTGGGGACACCAT
  CCTCATCAACAACCGCCCAGGGCCCCGAGAGCCACCCCCTTACCAGGAACCCCGGCCTCGGGGGACTCCA
  ACTCACTCTGCGCCCTGCGTCCCCAATGGCTCTGCGTTGCTGCTCTCCAATCCGGCCTACCGCCTCCTTC
  TGGCCACTTACGCCCGTCCCCCTCGAGGCCCGGGCCCCCCCACACCCGCCTGGGCCAAACCCACCAACAC
  CCAGGCCTGCAGTGGGGACTACATGGAGCCCGAGAAGCCGGGCGCCCCACTTCTACCCCCACCTCCCCAG
  AACAGCGTCCCCCATTACGCCGAGGCTGACATTGTCACCCTGCAGGGCGTCACAGGGGGCAACACCTACG
  CTGTTCCCGCACTGCCCCCAGGGGCGGTTGGGGATGGGCCCCCCAGAGTGGATTTCCCTCGGTCACGGCT
  CCGTTTCAAGGAGAAGCTCGGCGAGGGCCAATTTGGGGAGGTACATCTCTGTGAAGTAGAGGACCCTCAA
  GATCTGGTCACTAGTGACTTCCCTATCAGTGTGCAAAAGGGACACCCCTTGCTGGTGGCAGTGAAGATCC
  TCCGGCCAGATGCCACCAAAAATGCCAGGAATGACTTCCTGAAGGAGGTAAAGATCATGTCACGGCTGAA
  GGACCCAAACATCATCCGGCTCCTGGGTGTGTGTGTGCAGGATGACCCTCTCTGCATGATTACAGACTAC
  ATGGAGAACGGTGACCTGAACCAATTCCTCAGTGCCCACCAGCTGGAGAACAAGGTCACTCAGGGGCTTC
  CTGGGGACAGAGAGTCTGACCAGGGGCCCACAATCAGCTACCCTATGCTGTTACACGTGGGGGCCCAGAT
  CGCCTCTGGCATGCGATATCTGGCCACCCTTAACTTTGTGCATCGGGACCTGGCCACCCGGAACTGCTTG
  GTTGGGGAAAATTTCACCATCAAAATCGCCGACTTTGGCATGAGCCGGAATCTCTACGCTGGGGATTATT
  ACCGTGTACAGGGCCGGGCAGTGCTGCCCATCAGGTGGATGGCCTGGGAGTGTATCCTCATGGGGAAGTT
  CACAACAGCCAGTGATGTGTGGGCCTTCGGAGTGACCCTGTGGGAGGTGCTTATGCTCTGCAGGTCACAG
  CCCTTCGGGCAACTCACAGACGAGCAGGTCATCGAGAACGCCGGCGAGTTCTTCAGGGACCAGGGCCGGC
  AGGTATACTTATCCAGGCCACCCGCCTGCCCGCAGACCTTGTATGAGCTGATGCTCCGGTGTTGGAGCCG
  GGAGCCGGAGCAGCGGCCGCCCTTCTCCCAGCTTCATCGGTTCCTGGCGGATGATGCACTCAACACAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213471 representing NM_013137
  Red=Cloning site Green=Tags(s)
  
  MGTGTLSSLLLLLLLVTIGDADMKGHFDPAKCRYALGMQDRTIPDSDISVSSSWSDSTAARHSRLESSDG
  DGAWCPAGPVFPKEEEYLQVDLRRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMDWKDRWGQE
  VISGNEDPGGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMQLSEMVY
  LNDSTYDGYTAGGLQYGGLGQLADGVVGLDDFRQSQELRVWPGYDYVGWSNHSFPSGYVEMEFEFDRLRS
  FQTMQVHCNNMHTLGARLPGGVECRFKRGPAMAWEGEPVRHALGGSLGDPRARAISVPLGGHVGRFLQCR
  FLFAGPWLLFSEISFISDVVNDSSDTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTAILIGCL
  VAIILLLLLIIALMLWRLHWRRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGTP
  THSAPCVPNGSALLLSNPAYRLLLATYARPPRGPGPPTPAWAKPTNTQACSGDYMEPEKPGAPLLPPPPQ
  NSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDGPPRVDFPRSRLRFKEKLGEGQFGEVHLCEVEDPQ
  DLVTSDFPISVQKGHPLLVAVKILRPDATKNARNDFLKEVKIMSRLKDPNIIRLLGVCVQDDPLCMITDY
  MENGDLNQFLSAHQLENKVTQGLPGDRESDQGPTISYPMLLHVGAQIASGMRYLATLNFVHRDLATRNCL
  VGENFTIKIADFGMSRNLYAGDYYRVQGRAVLPIRWMAWECILMGKFTTASDVWAFGVTLWEVLMLCRSQ
  PFGQLTDEQVIENAGEFFRDQGRQVYLSRPPACPQTLYELMLRCWSREPEQRPPFSQLHRFLADDALNTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013137
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013137.2, NP_037269.2
• RefSeq Size: 2921 bp
• RefSeq ORF: 2733 bp
• Locus ID: 25678
• Cytogenetics: 20p12
• MW: 101.2 kDa
• Gene Summary: protein tyrosine kinase that may play a role in nervous system development [RGD, Feb 2006]