Taok3 (NM_001024254) Rat Tagged ORF Clone

CAT#: RR213390

Taok3 (Myc-DDK-tagged ORF) - Rat TAO kinase 3 (Taok3), (10 ug)

Product Images

Circular map for RR213390

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taok3
• Synonyms: JIK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213390 representing NM_001024254
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTAAAGGAGTGCTGAAGGACCCCGAGATTGCCGAGCTGTTCTTCAAGGAGGACCCCGAAGAGCTCT
  TTATCGACCTGCATGAAATCGGACATGGCAGCTTTGGAGCCGTGTACTTCGCAACTAATGCGCACACCAA
  CGAGGTGGTTGCTATTAAGAAAATGTCCTACAGTGGGAAGCAGACCCACGAGAAATGGCAAGATATCCTG
  AAGGAAGTTCGATTCCTCCAGCAGCTGAAGCACCCGAACACCATCGAGTACAAAGGCTGTTATTTAAAGG
  AGCACACCGCTTGGTTGGTGATGGAGTACTGCTTAGGTTCAGCCTCTGACTTGTTAGAAGTTCATAAGAA
  ACCACTTCAGGAAGTAGAGATTGCTGCTATCACCCATGGTGCCTTGCAGGGGCTGGCCTACCTCCACTTT
  CACTCCCTGATTCACAGGGACATTAAAGCAGGGAATATCCTCCTCACAGAGCCAGGCCAGGTGAAGCTGG
  CTGACTTCGGGTCCGCTTCCATGGCTTCTCCTGCCAACTCCTTCGTGGGGACTCCTTACTGGATGGCCCC
  AGAGGTGATCTTAGCCATGGACGAAGGGCAGTACGACGGGAAAGTTGACATCTGGTCACTCGGCATCACC
  TGCATAGAGCTGGCGGAGCGGAAGCCCCCCCTCTTCAACATGAACGCCATGAGCGCCCTCTACCACATCG
  CTCAGAATGACTCCCCCACGCTGCAGTCCAGAGAATGGACAGACTCCTTCAGGAGATTCGTTGATTACTG
  CTTGCACAAAATACCTCAGGAACGACCAGCCGCGGCGGAACTGTTGCGGCACGACTTCATTCGGAGGGAG
  CGGCCGCCGAGGGTCCTCATTGACCTCATACAGAGGACGAAAGATGCTGTCCGAGAGCTGGACAACCTGC
  AGTACAGGAAAATGAAGAAGATCCTCTTCCAGGAGACTCGGAACGGACCCCTGAATGAGTCACAAGAAGA
  GGAAGAAGACAGTGAGCAAGGAAGCAACCTGAACCGAGAGGTGGACAGTCTGGGGAGCATCCACTCCATC
  CCCAGCGTGTCAGTGAGCACGGGCAGCCGGAGCAGCAGCGTGAACAGCATGCAGGAGGTCATGGACGAGG
  GCAGCCCTGAGCTCGTCATGATGCAGGAGGATGAAGGCACAGTCAACTCCAGCTCCTCTATGGTACACAA
  GAAGGATCATGTATTCGTAAGGGATGAGGCGGGCCACGGCGATCCCAGGCCTGAGCCGCGGCCTACCCAG
  TCAGTTCAGAGCCGGGCCCTCCACTATCGGAACCGAGAGCGCTTTGCCACGATCAAATCCGCGTCTTTGG
  TTACACGGCAGATCCACGAGCATGAGCAGGAGAACGAGCTGCGGGAACAGATGTCAGGTTATAAGCGGAT
  GCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAACAAGCTGAAGGCTGAGATGGATGAGCACCGC
  CTCAAGCTCCAGAAGGAGGTGGAGACGCATGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGA
  AGCAAGTGGCTACCATAGAAAAGGAGGCAAAGGTAGCCGCAGCGGATGAGAAGAAGTTCCAGCAGCAGAT
  CCTGGCCCAGCAGAAGAAGGACTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTACAAGATTTGTAAG
  GAGAAAATAAAAGAGGAAATGAACGAGGACCATAGCACACCCAAGAAGGAGAAGCAGGAGCGCATATCGA
  AGCATAAAGAGAACCTGCAGCACACGCAGGCCGAGGAGGAAGCCCACCTGCTCACGCAACAGAGGCTGTA
  CTACGACAGAAACTGCCGCTGCTTCAAGCGGAAAATAATGACCAAGCGCCACGAGGTGGAACAGCAGAAC
  ATTCGGGAGGAACTAAATAAAAAGAGGACCCAGAAAGAAATGGAGCACGCCATGCTGATCCGGCATGATG
  AATCCACCCGAGAGCTCGAGTACAGACAGCTGCACACACTGCAGAAGCTCCGCATGGACCTGATCCGGTT
  ACAGCACCAGACTGAGCTGGAGAACCAGCTGGAGTACAATAAGAGGCGGGAGCGGGAGCTGCACCGGAAG
  CACGTCATGGAGCTTCGGCAACAGCCGAAAAACTTAAAGGCCATGGAAATGCAAATTAAAAAGCAGTTTC
  AGGACACTTGCAAAGTACAGACCAAACAGTACAAAGCACTCAAAAACCACCAGTTGGAAGTCACTCCAAA
  GAATGAGCACAAAGCAATCCTAAAGACACTGAAGGAGGAGCAGACGAGGAAGCTGGCCATCTTGGCCGAG
  CAGTATGAACAGAGTATAAACGAGATGATGGCCTCTCAAGCGTTACGGCTAGACGAGGCGCAAGAGGCAG
  AATGCCAGGCCTTGAGACTACAGCTCCAGCAGGAGATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAA
  GATGCAAACGGAGGCCCAGCACGAGCGCGAGCTGCAGAAGCTAGAGCAGAGGGTGTCCCTGCGCAGAGCG
  CATCTTGAGCAGAAGATTGAAGAGGAGCTGGCCGCCCTGCAGAAGGAACGCAGTGAGAGGATCAAGACTC
  TCCTGGAAAGACAAGAGCGAGAGACCGAGACTTTTGACATGGAGAGCCTCAGAATGGGGTTTGGGAATTT
  GGTGACATTAGATTTTCCTAAGGAGGACTATAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213390 representing NM_001024254
  Red=Cloning site Green=Tags(s)
  
  MRKGVLKDPEIAELFFKEDPEELFIDLHEIGHGSFGAVYFATNAHTNEVVAIKKMSYSGKQTHEKWQDIL
  KEVRFLQQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALQGLAYLHF
  HSLIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGIT
  CIELAERKPPLFNMNAMSALYHIAQNDSPTLQSREWTDSFRRFVDYCLHKIPQERPAAAELLRHDFIRRE
  RPPRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEEEEDSEQGSNLNREVDSLGSIHSI
  PSVSVSTGSRSSSVNSMQEVMDEGSPELVMMQEDEGTVNSSSSMVHKKDHVFVRDEAGHGDPRPEPRPTQ
  SVQSRALHYRNRERFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHR
  LKLQKEVETHANNSSIELEKLAKKQVATIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICK
  EKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLTQQRLYYDRNCRCFKRKIMTKRHEVEQQN
  IREELNKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRERELHRK
  HVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKAILKTLKEEQTRKLAILAE
  QYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAYQSKIKMQTEAQHERELQKLEQRVSLRRA
  HLEQKIEEELAALQKERSERIKTLLERQERETETFDMESLRMGFGNLVTLDFPKEDYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024254
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024254.1, NP_001019425.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 2697 bp
• Locus ID: 304530
• UniProt ID: Q53UA7
• Cytogenetics: 12q16
• MW: 105.5 kDa
• Gene Summary: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF) (By similarity).[UniProtKB/Swiss-Prot Function]