Ptk7 (NM_001106889) Rat Tagged ORF Clone

CAT#: RR213149

Ptk7 (Myc-DDK-tagged ORF) - Rat PTK7 protein tyrosine kinase 7 (Ptk7), (10 ug)

Product Images

Circular map for RR213149

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptk7
• Synonyms: Srf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213149 representing NM_001106889
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCTTTGCCAGGGTGCTGCTGGCACCTCAGGACGTCGTTGTGGCAAGGAATGAGGAGGCCATGTT
  CCATTGCCAGTTTTCAGCTCAGCCGCCCCCGAGCCTGCAGTGGGTCTTTGAGGATGAGACTCCCATCACG
  AACCGAAGCCGCCATGGTGTTTGCCAATGGCTCCCTGCTGCTGACCCAAGTCCGGCCACGGAACGCAGGA
  GTCTACCGTTGCATTGGTCAGGGACAGAGGGGTCCCCCCATCGTCCTAGAAGCCACTCTTCACCTGGCAG
  AGATCGAAGACATGCTGCCGTTGGAACCACGGGTGTTCATAGCTGGTGGTGAGGAACGTGTTGCCTGCCC
  AGCCCCCCAGGGTCTGCCTACGCCCAGTGTCTGGTGGGAACACGCTGGAGTCCGTCTTCCTGCTCATGGA
  AGGGTCCACCAGAAGGGCCTCGAGCTCGTGTTTGCCACTATCGCTGAGAGTGACGCCGGTGTCTACACCT
  GCCACGCAGCCAACCTGGCTGGTCAGCGGCGACAGGATGTGAACATCACAGTGGCCACTGTTCCCACGTG
  GTTAAGGAAGCCCCAAGACAGCCAGCTGGAAGAGGGCAAACCTGGCTACCTGCACTGTCTGACACAGGCC
  ACACCCAAGCCCACCGTCATCTGGTACAGGAACCAGATGCTTATCTCGGAGGACTCACGCTTCGAAGTCT
  CAAAGAATGGGACCTTGCGCATCAACAGTGTGGAGGTATATGACGGGACAGTGTACCGCTGTGTGAGCAG
  CACCCCAGCTGGCAGCATCGAGGCCCAGGCCCGTGTCCAGGTGCTGGAAAAACTCAAGTTCACGCCACCG
  CCACAGCCACAGCAATGCATGGAGTTTGATAAGGAGGCTACGGTGCCCTGCTCAGCCACTGGCCGAGAGA
  AACCCACGGTTAAGTGGGTACGGGCAGATGGGAGCAGCCTCCCCGAATGGGTGACAGACAATGCGGGGAC
  CCTGCACTTTGCCCGAGTGACCCGAGATGACGCTGGCAACTACACCTGCATTGCGTCCAATGAGCCACAG
  GGCCAGATCCGTGCCCATGTCCAGCTGACGGTGGCAGTTTTCATCACGTTCAAGGTGGAACCGGAGCGTA
  CAACGGTGTACCAGGGCCACACGGCGCTGCTGCGGTGTGAGGCGCAGGGGGATCCCAAGCCTCTTATTCA
  GTGGAAGGGCAAAGACCGAATCCTGGACCCCACCAAGCTGGGACCCAGGATGCACATCTTCCAGAACGGC
  TCCCTGGTGATCCATGATGTGGCCCCTGAGGACTCAGGCTCCTACACCTGCATCGCTGGCAACAGCTGTA
  ACATCAGGCACACAGAGGCCCCGCTCTTAGTCGTGGGTGGGCCTTTGCAGAATGGACAGCCGTCAGCAGA
  GATCCAGGAAGAAGTGGCGTTGACCAGCTTGGGCTCTGGCCCTACAGCCACCAACAAGCGCCACAGCACT
  GGCGATAAGATGCATTTCCCTCGGGCCAGCCTGCAGCCCATCACCACTCTGGGGAAAAGCGAGTTCGGGG
  AGGTGTTCCTCGCAAAGGCTCAGGGCTTAGACGAAGGCGCGACAGAGACCCTGGTGCTTGTGAAAAGCCT
  GCAGAACAAGGACGAGCAGCAGCAGCTGGACTTCCGGAGGGAGTTTGAGATGTTTGGGAAACTGAACCAT
  GCCAACGTGGTGCGGCTCCTGGGGCTGTGCCGGGAGGCTGAGCCCCATTACATGGTGCTGGAGTATGTGG
  ACCTGGGAGATCTCAAACAGTTCCTGAGGATTTCCAAGAACAAGGATGAAAAGTTGAAGTCACAGCCCCT
  CAGCACCAAACAGAAGGTGGCCCTGTGTTCCCAGGTGGCCCTGGGCATGGAACACTTGTCCAACAACCGC
  TTTGTGCACAAGGACTTAGCTGCTCGCAACTGCCTGGTCAGCGCCCAGAGGCAGGTGAAGGTGTCCGCGC
  TGGGCCTCAGCAAGGATGTATACAACAGTGAATACTACCACTTCCGCCAGGCCTGGGTACCTTTGCGTTG
  GATGTCCCCAGAGGCTGTCCTGGAGGGTGACTTCTCCACCAAGTCTGACGTCTGGGCCTTTGGCGTGCTC
  ATGTGGGAAGTGTTCACTCATGGAGAGATGCCCCATGGTGGACAGGGAGATGATGAGGTGCTGGCGGACT
  TGCAGGCTGGGAAGGCTCGGCTCCCGCAGCCCGAAGGCTGCCCGTCTAAGCTCTACCGGCTGATGCAACG
  CTGCTGGGCCCTGAACCCCAAGGACCGGCCCTCCTTCAGCGAGATCGCTAGCACCCTGGGAGATGGCCCT
  GCAGACAGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213149 representing NM_001106889
  Red=Cloning site Green=Tags(s)
  
  MRALPGCCWHLRTSLWQGMRRPCSIASFQLSRPRACSGSLRMRLPSRTEAAMVFANGSLLLTQVRPRNAG
  VYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHG
  RVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQA
  TPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPP
  PQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQ
  GQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNG
  SLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVGGPLQNGQPSAEIQEEVALTSLGSGPTATNKRHST
  GDKMHFPRASLQPITTLGKSEFGEVFLAKAQGLDEGATETLVLVKSLQNKDEQQQLDFRREFEMFGKLNH
  ANVVRLLGLCREAEPHYMVLEYVDLGDLKQFLRISKNKDEKLKSQPLSTKQKVALCSQVALGMEHLSNNR
  FVHKDLAARNCLVSAQRQVKVSALGLSKDVYNSEYYHFRQAWVPLRWMSPEAVLEGDFSTKSDVWAFGVL
  MWEVFTHGEMPHGGQGDDEVLADLQAGKARLPQPEGCPSKLYRLMQRCWALNPKDRPSFSEIASTLGDGP
  ADSKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106889
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106889.1, NP_001100359.1
• RefSeq Size: 3934 bp
• RefSeq ORF: 2328 bp
• Locus ID: 301242
• Cytogenetics: 9q12
• MW: 85.9 kDa