Kl (NM_031336) Rat Tagged ORF Clone

CAT#: RR213144

Kl (Myc-DDK-tagged ORF) - Rat Klotho (Kl), (10 ug)

Product Images

Circular map for RR213144

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213144 representing NM_031336
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCCGCGCCCCTCCTCGCCGCCTGCCGCGGCTCTTGCTGCTCCGTTTGCTGTCGCTGCATCTGC
  TGTTGCTCACCCTGCGCGCCCGCTGCCTAAGCGCTGAGCCGGGTCAGGGCGCGCAGACCTGGGCTCGCTT
  TGCGCGCCCTCCTGTCCCAGAGGCCTCTGGCCTCCTCCATGACACCTTCCCCGATGGTTTCCTCTGGGCG
  GTAGGCAGCGCCGCCTACCAGACGGAGGGCGGCTGGCGACAGCACGGCAAAGGCGCGTCCATCTGGGACA
  CTTTCACCCATCACCCTCGGGCCATCCCGGAGGACTCCCCGATCGTCATGGCGCCGTCGGGTGCCCCGTT
  GCCTCCCCTGCCCTCCACTGGAGATGTGGCCAGCGACAGTTACAACAACGTCTACCGCGACACAGAGGGG
  CTACGCGAACTGGGGGTCACCCACTACCGCTTCTCCATATCGTGGGCGCGGGTGCTCCCCAATGGCACCG
  CGGGCACCCCCAACCGCGAGGGGCTGCGCTACTACCGGCGGCTGCTGGAGCGGCTGAGGGAGCTGGGCGT
  GCAGCCGGTGGTCACTCTGTACCATTGGGACCTGCCGCAGCGCCTGCAGGACACCTATGGCGGCTGGGCC
  AATCGCGCCCTGGCCGACCATTTCAGGGATTACGCCGAGCTCTGCTTCCGCCACTTCGGTGGTCAGGTCA
  AGTACTGGATCACCATTGACAACCCCTACGTGGTGGCCTGGCACGGGTACGCCACCGGCCGCCTGGCCCC
  GGGCGTGCGGGGCAGCTCCAGGCTCGGGTACCTGGTTGCCCACAACCTACTTTTGGCTCACGCCAAAGTC
  TGGCGTCTCTACAACACCTCCTTCCGCCCCACTCAGGGGGGCCGGGTGTCCATCGCCTTAGGTTCCCATT
  GGATCACCCCTCGAAGGATGACCGACTATCACATCAGAGAGTGTCAGAAGTCTCTTGACTTCGTGCTAGG
  CTGGTTTGCCAAGCCCATATTTATCGATGGCGACTACCCAAAGAGTATGAAGAACAACCTCTCATCCCTG
  CTGCCCGATTTTACTGAATCCGAGAAGAGATTCATCAGGGGAACAGCTGACTTCTTTGCTCTCTCCTTCG
  GACCAACCTTGAGCTTTCAACTACTGGACCCCAGCATGAAGTTCCGCCAGCTGGAGTCTCCCAGCCTGAG
  GCAGCTTCTGTCTTGGATAGATCTGGAGTATAACCACCCTCAGATATTTATTGTAGAAAATGGCTGGTTT
  GTCTCGGGGACCACCAGAAGAGATGATGCCAAATACATGTATTATCTCAAGAAGTTCATAATGGAAAGCT
  TAAAAGCCATCAGGCTGGATGGGGTCGACGTCATTGGGTACACCGCATGGTCCCTCATGGATGGTTTCGA
  GTGGCACAGGGGCTACAGCATCCGACGAGGACTCTTCTATGTCGACTTTCTGAGTCAGGACAAGGAGTTG
  TTGCCCAAGTCTTCGGCCTTGTTCTACCAAAAGCTGATAGAGAACAATGGCTTCCCTCCTTTACCTGAGA
  ACCAGCCCCTTGAAGGGACGTTTCCCTGTGACTTTGCTTGGGGAGTGGTTGACAACTACATTCAAGTGGA
  CCCCACTCTCTCTCAGTTTACTGACCCGAATGTCTATCTGTGGGATGTGCATCACAGTAAGAGGCTTATT
  AAAGTAGACGGGGTTGTAGCCAAGAAGAGAAAACCTTACTGTGTTGATTTCTCTGCCATCCGGCCTCAGA
  TAACCTTACTTCGAGAAATGCGGGTCACCCACTTTCGGTTCTCCCTAGACTGGGCCCTGATCTTGCCTCT
  GGGTAACCAGACTCAAGTGAACCGCACGGTTCTGCACTTCTACCGCTGTATGGTCAGCGAGCTGGTGCAC
  GCCAACATCACTCCAGTGGTGGCCCTGTGGCAACCAGCAACCCCACACCAAGGCCTGCCACATGCCCTTG
  CAAAACATGGGGCCTGGGAGAACCCGCACACTGCCCTGGCATTTGCAGACTACGCAAACCTGTGCTTTGA
  AGAGCTAGGCCACTGGGTCAAGTTCTGGATCACCATAAATGAGCCAAACTCACGGAACATGACATACCGT
  GCGGGGCACCACCTGCTTAAGGCTCATGCCTTGGCATGGCACCTGTACGATGACAAATTTAGGGCGGCTC
  AGAAAGGCAAAATATCCATCGCCTTGCAGGTTGACTGGATAGAACCGGCCTGCCCATTCTCTCAGAAGGA
  CAAAGAAGTGGCGGAGAGAGTTTTGGAATTTGATGTAGGCTGGCTGGCAGAGCCCATTTTCGGCTCCGGA
  GATTATCCGCATGTGATGAGGGAATGGCTGAACCAAAAAAACAACTTTCTTCTGCCCTATTTCACGGAAG
  ATGAGAAAAAGCTAATCCGGGGTTCCTTTGACTTCCTGGCGTTGAGCCATTACACCACCATCCTTGTAGA
  CTGGGAAAAGGAGGATCCGATAAAATACAACGATTACCTGGAGGTGCAGGAGATGACTGACATCACGTGG
  CTCAACTCCCCCAATCAGGTGGCGGTGGTGCCTTGGGGGCTGCGCAAAGCGCTCAACTGGCTAAGGTTCA
  AGTATGGAGACCTCCCGATGTTCGTGACAGCCAATGGCATTGATGACGATCCCCATGCCGAGCAAGACTC
  ACTGAGGATGTATTATATTAAGAATTACGTGAATGAGGCTCTGAAAGCCTACGTGTTGGACGGCATCAAC
  CTTTGTGGCTACTTTGCGTATTCGCTTAGTGACCGCTCAGTTCCCAAGTCCGGCTTTTATCGATACGCTG
  CGAATCAGTTCGAGCCCAAACCGTCTATTAAACATTACAGGAAAATTATTGACAACAACGGCTTCCTGGG
  TTCTGGAACGCTGGGAAGGTTTTGTCCGGAAGAGTACACCGTGTGCACCGGATGCGGCTTTTTTCAAACC
  CGGAAGTCCCTGCTGGCCTTCATCTCGTTTCTAGTTTTTGCTTTCGTTACTTCTCTCGCTCTTATTTATT
  ACTACTCCAAGAAAGGCCGGAGACGTTATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213144 representing NM_031336
  Red=Cloning site Green=Tags(s)
  
  MPARAPPRRLPRLLLLRLLSLHLLLLTLRARCLSAEPGQGAQTWARFARPPVPEASGLLHDTFPDGFLWA
  VGSAAYQTEGGWRQHGKGASIWDTFTHHPRAIPEDSPIVMAPSGAPLPPLPSTGDVASDSYNNVYRDTEG
  LRELGVTHYRFSISWARVLPNGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWA
  NRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLAHAKV
  WRLYNTSFRPTQGGRVSIALGSHWITPRRMTDYHIRECQKSLDFVLGWFAKPIFIDGDYPKSMKNNLSSL
  LPDFTESEKRFIRGTADFFALSFGPTLSFQLLDPSMKFRQLESPSLRQLLSWIDLEYNHPQIFIVENGWF
  VSGTTRRDDAKYMYYLKKFIMESLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKEL
  LPKSSALFYQKLIENNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDPTLSQFTDPNVYLWDVHHSKRLI
  KVDGVVAKKRKPYCVDFSAIRPQITLLREMRVTHFRFSLDWALILPLGNQTQVNRTVLHFYRCMVSELVH
  ANITPVVALWQPATPHQGLPHALAKHGAWENPHTALAFADYANLCFEELGHWVKFWITINEPNSRNMTYR
  AGHHLLKAHALAWHLYDDKFRAAQKGKISIALQVDWIEPACPFSQKDKEVAERVLEFDVGWLAEPIFGSG
  DYPHVMREWLNQKNNFLLPYFTEDEKKLIRGSFDFLALSHYTTILVDWEKEDPIKYNDYLEVQEMTDITW
  LNSPNQVAVVPWGLRKALNWLRFKYGDLPMFVTANGIDDDPHAEQDSLRMYYIKNYVNEALKAYVLDGIN
  LCGYFAYSLSDRSVPKSGFYRYAANQFEPKPSIKHYRKIIDNNGFLGSGTLGRFCPEEYTVCTGCGFFQT
  RKSLLAFISFLVFAFVTSLALIYYYSKKGRRRYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031336
• ORF Size: 3042 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031336.1, NP_112626.1
• RefSeq Size: 3525 bp
• RefSeq ORF: 3045 bp
• Locus ID: 83504
• UniProt ID: Q9Z2Y9
• Cytogenetics: 12p12
• MW: 116.8 kDa
• Gene Summary: may be involved in suprresion of aging phenotypes [RGD, Feb 2006]