Csf1 (NM_023981) Rat Tagged ORF Clone

CAT#: RR212926

Csf1 (Myc-DDK-tagged ORF) - Rat colony stimulating factor 1 (macrophage) (Csf1), (10 ug)

Product Images

Circular map for RR212926

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212926 representing NM_023981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCGCGGGGCGCCGCGGGGCGCTGCCCTTCTTCGACATGGATGGGCTCCCGGCTGCTGCTGGTCT
  GTCTCCTCGTGAGCAGGAGTGTTGCCGAGGTGTCGGAGCACTGTAGCCACATGATTGGGAATGGACACCT
  ACAGATTTTGCAGCAGTTGATCGACAGCCAAATGGAGACTGCATGCCTGATCGAATATAAATTTGTAGAC
  CAGGAACAGCTGGACGATCCCGTTTGCTACCTAAAGAAGGCCTTTGTTTTGGTACAAGTCATAATCGAGG
  AAACCATGCGCTTTAAAGACAACACCCCCAACGCCAACGCCACCGAGAGGCTACAGGAACTCTCTATGAA
  ACTGAACAGCTGCTTTATCAAGGACTATAAGGAACAGAACGAGGCCTGTGTCCAAACTTACAAAGAGAGT
  CCTCTCCGGTTGCTGGAGAAAATCAAGAATTTCTTTAACGAAACAAAGAATTTCCTTGAAAAGGACTGGA
  ATATTTTTAGCAAGAACTGCAATGACAGCCTTGCTAAATGCTCTAGCAGAGATGTGGTGACCAAGCCTGA
  TTGCAACTGCCTGTACCCTAAAGCCACCCCTAGCAGCGACCTGGCCTCTGCCTCCCCTCACCAGCCCCCT
  GCCCCCTCCATGGCCCCTCTGGCTGACTTGGCTTGGGATGATTCTCAGAGGACAGAGGGAAGCTCCCTCT
  TGCCCAGTGATCTGCCTCTTCGCATAGAGGACCCAGGCAGTGCTAAGCAGCGACCGCCCAGGAGTACCTG
  CCAGACCCTCGAGTCAACAGAGCAACCAAATCACGAGGACCCACAACCTCATCCTTCTGCGGGGGCCCCC
  ATCCCTGGGGTGGAAGACATTATTGAATCTTCAATGGGCACTAACTGGGTCTTAGAAGAAGCTTCTGGAG
  AGGCTAGTGAGGGATTTTTGACCCAGGAAAGGAAGTTTTCCCCCTCCAATCCTGTAGGGGGCAGCATCCA
  GGCAGAGACTGACAGACCCTGGGCCCGCTCAGCATCTTCTCCATTCCCTAAATTAACAGAGGATCAACAG
  CCAACGAATATAACAGACACCCCGTTGACAGAGGTGAACCCTATGAGACCCACTGGCCAGACACTGAATA
  ATACTCCTGAGAAGACGGATGGTTCATCTACACTGCGCGAAGACCAGCAGGAGCCACGCTCTCCCCATTT
  TGCCACACTGAATCCCCAACGAGTCGGCAACTCAGCCACCCCCTATGCTAAGTTACTGCCTCCCAAAAGC
  CACTCTTGGGGCATTGTACTGCCCCTTGGGGAGCTCGAGGGCAAGAAAAGTACTAGAGATCGAAGGAGCC
  CCGCAGAGCTGAAAGGAGGACCAGCAAGTGAGGGGGCAGCCAGGCCTGTGGCCCAAAGTACTAGAGATCG
  AAGGAGCCCCGCAGAGCTGAAAGGAGGACCAGCAAGTGAGGGGGCAGCCAGGCCTGTGGCCCGTTTTAAT
  TCCATTCCTTTGACCGACACAGGCTCTTCTATTCAGGATCCCCAGACCTCTGCGTTTGTCTTCTGGGTGC
  TAGGCATCATCCTAGTCTTGCTGGCTGTCGGGGGCCTCCTGTTCTACAGTTGGAAGCGGAGGAGCCATCG
  AGACCCTCGGACATTGGATTCTTCTGTGGGGCGACCAGAGGGCAGCTCCCTGGCCCAGGATGAGGACAGA
  CAGGTGGAACTGCCAGTGCAGGTGGAACTGCCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212926 representing NM_023981
  Red=Cloning site Green=Tags(s)
  
  MTARGAAGRCPSSTWMGSRLLLVCLLVSRSVAEVSEHCSHMIGNGHLQILQQLIDSQMETACLIEYKFVD
  QEQLDDPVCYLKKAFVLVQVIIEETMRFKDNTPNANATERLQELSMKLNSCFIKDYKEQNEACVQTYKES
  PLRLLEKIKNFFNETKNFLEKDWNIFSKNCNDSLAKCSSRDVVTKPDCNCLYPKATPSSDLASASPHQPP
  APSMAPLADLAWDDSQRTEGSSLLPSDLPLRIEDPGSAKQRPPRSTCQTLESTEQPNHEDPQPHPSAGAP
  IPGVEDIIESSMGTNWVLEEASGEASEGFLTQERKFSPSNPVGGSIQAETDRPWARSASSPFPKLTEDQQ
  PTNITDTPLTEVNPMRPTGQTLNNTPEKTDGSSTLREDQQEPRSPHFATLNPQRVGNSATPYAKLLPPKS
  HSWGIVLPLGELEGKKSTRDRRSPAELKGGPASEGAARPVAQSTRDRRSPAELKGGPASEGAARPVARFN
  SIPLTDTGSSIQDPQTSAFVFWVLGIILVLLAVGGLLFYSWKRRSHRDPRTLDSSVGRPEGSSLAQDEDR
  QVELPVQVELPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023981
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3975 bp
• RefSeq ORF: 1701 bp
• Locus ID: 78965
• Cytogenetics: 2q34
• MW: 62.2 kDa
• Gene Summary: plays a role in macrophage formation; involved in osteoclastogenesis and endochondral ossification [RGD, Feb 2006]