Gabrp (NM_031029) Rat Tagged ORF Clone

CAT#: RR212766

Gabrp (Myc-DDK-tagged ORF) - Rat gamma-aminobutyric acid (GABA-A) receptor, pi (Gabrp), (10 ug)

Product Images

Circular map for RR212766

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gabrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212766 representing NM_031029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACAGTCTCTATTTGGCCTTTGTGTGTCTGAATCTCCTCGCTCAAAGGATGTGCATCCAGGGGA
  ACCAGTTCAATGTGGAGGTCAGCAGAAGCGACAAGCTTTCCTTGCCTGGATTTGAGAACCTCACTGCAGG
  ATATAACAAATTCCTCAGGCCCAATTTTGGTGGTGACCCAGTTCGGATAGCGCTGACTCTGGACATCGCA
  AGCATCTCTAGTATTTCAGAGAGTAACATGGACTACACAGCCACTATATACCTCCGACAGCGCTGGACCG
  ACCCACGGCTGGTGTTCGAAGGCAACAAGAGCTTTACTCTAGATGCCCGTCTCGTGGAGTTCCTCTGGGT
  GCCAGATACTTACATCGTGGAGTCCAAGAAGTCCTTCCTGCATGAAGTCACGGTGGGAAACAGGCTCATC
  CGCCTCTTCTCCAACGGCACAGTCTTGTATGCACTCAGAATCACAACAACAGTTACATGTAACATGGATC
  TGTCGAAATACCCCATGGACACACAGACCTGCAAGTTGCAGCTGGAAAGTTGGGGATACGATGGCAATGA
  CGTGGAGTTCTCCTGGCTAAGAGGGAACGATTCGGTACGTGGACTCGAAAACCTGCGCCTTGCTCAGTAC
  ACCATCCAACAGTATTTCACCCTGGTCACCGTATCCCAGCAGGAGACAGGAAACTATACGCGGTTGGTTT
  TGCAATTTGAGCTTCGGAGGAATGTCCTGTATTTCATTCTGGAAACCTACGTCCCTTCCACCTTCCTGGT
  GGTGTTATCCTGGGTCTCCTTTTGGATCTCTCTCGAGTCGGTTCCTGCGAGAACCTGCATTGGAGTGACA
  ACAGTGTTGTCCATGACAACGCTGATGATTGGCTCCCGCACATCCCTACCCAACACCAACTGCTTCATAA
  AGGCCATTGATGTTTACCTGGGGATTTGCTTCAGCTTTGTGTTTGGGGCCTTACTGGAGTATGCAGTTGC
  TCACTACAGCTCCTTACAGCAGATGGCAGTCAAAGATAGGGGGCCAGCAAAGGACTCAGAAGAAGTCAAT
  ATCACCAACATCATCAACAGCTCCATCTCCAGCTTTAAACGAAAGATCAGCTTCGCCAGCATTGAAATTT
  CTGGCGACAATGTCAACTACAGCGACCTGACGATGAAAGCCAGCGACAAGTTTAAGTTTGTCTTCCGAGA
  AAAGATCGGCAGAATCATTGATTACTTCACAATTCAAAATCCCAGCAACGTGGATCGATATTCCAAACTA
  CTGTTTCCTTTGATTTTTATGTTGGCCAATGTATTTTACTGGGCATACTACATGTATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212766 representing NM_031029
  Red=Cloning site Green=Tags(s)
  
  MSYSLYLAFVCLNLLAQRMCIQGNQFNVEVSRSDKLSLPGFENLTAGYNKFLRPNFGGDPVRIALTLDIA
  SISSISESNMDYTATIYLRQRWTDPRLVFEGNKSFTLDARLVEFLWVPDTYIVESKKSFLHEVTVGNRLI
  RLFSNGTVLYALRITTTVTCNMDLSKYPMDTQTCKLQLESWGYDGNDVEFSWLRGNDSVRGLENLRLAQY
  TIQQYFTLVTVSQQETGNYTRLVLQFELRRNVLYFILETYVPSTFLVVLSWVSFWISLESVPARTCIGVT
  TVLSMTTLMIGSRTSLPNTNCFIKAIDVYLGICFSFVFGALLEYAVAHYSSLQQMAVKDRGPAKDSEEVN
  ITNIINSSISSFKRKISFASIEISGDNVNYSDLTMKASDKFKFVFREKIGRIIDYFTIQNPSNVDRYSKL
  LFPLIFMLANVFYWAYYMYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031029
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031029.1, NP_112291.1
• RefSeq Size: 1384 bp
• RefSeq ORF: 1323 bp
• Locus ID: 81658
• UniProt ID: O09028
• Cytogenetics: 10q12
• MW: 50.5 kDa
• Gene Summary: pi subunit of the GABA-A receptor; alters the sensitivity of GABA-A receptors to modulatory agents such as allopregnanolone [RGD, Feb 2006]