Ano6 (NM_001108108) Rat Tagged ORF Clone

CAT#: RR212483

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  • TrueORF®

Ano6 (Myc-DDK-tagged ORF) - Rat anoctamin 6 (Ano6), (10 ug)



  "NM_001108108" in other vectors (3)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,221.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Ano6
Synonyms Tmem16f
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR212483 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGATGATGACTAGGAACGTCCTGCTGAACATGGAGCTGGAAGAGGACGACGATGAGGATGGAGACA
TTGGTGATGTTCCTGACTCCAGGAGACCTTTTCTAGCTCCTCATACTCCTCTACCATCCGGTCTGGTGCT
GGAAAACTTCGACCAGACCATAGTCTGCTCCACCTTTGGATCACTGGAGAGCCAGGAGGACTTCCGCACT
CCAGAGTTTGAAGAGTTTAACGGAAAGCCTGACTCGCTCTTTTTCACCGATGGCCAGAGACGGATCGACT
TCGTCCTCGTGTACGAAGACGAGAGCAAGAAGGAGAACAATAAGAAAGGGACAAATGAGAAACAGAAGAG
GAAAAGGCAAGCATACGAATCTAACCTCATCAGCCACGGCCTGCAGCTGGAAGCCACAAGATCTGTTTCT
GATGACAAGCTTGTGTTCGTCAAAGTGCATGCGCCCTGGGAAGTGCTGTGCACCTATGCTGAGATCATGC
ACATCAAGCTCCCACTAAAGCCCAATGACCTGAAGACTCGTTCCACCTTTGACACCTTCAACTGGTTCAC
CAAGGTCCTCCGTGTGAACGAGAGTGTCATCAAGCCGGAGCAGGAGTTCTTCACTGCCCCCTTTGAGAAG
AGCCGGATGAATGACTTCTACATCCTTGACAGAGACTCCTTCTTCAATCCCGCCACCAGAAGTCGCATTG
TTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAATTTGGGATTAATAGACT
GGTCAGTTCTGGCATCTACAAAGCAGCGTTCCCTCTGCACGACTGCAAATTCAACTATAAGTCGGAAGAC
CCCAACTGTCCTAGCGAGCGTTACCTCCTGTACAGGGAATGGGCTCACCCTCGGAGTATATACAAGAAAC
AGCCCTTGGATCTTATCAGGAAGTATTACGGAGAGAAGATTGGAATCTACTTCGCGTGGCTGGGCTATTA
TACGCAGATGCTCCTTCTAGCAGCTGTGGTGGGCGTGGCCTGCTTCCTCTATGGATATCTTAATCAGGAT
AACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAGATCCTGATGTGTCCCCAGTGTG
ACAGGTTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCCTCTAAGGAGGAGGAGCGTATCCCATT
TACCACCTGTGGGAAGTGTATCCGGATCACCCTGTGTGCGAGCGCTGTCTTCTTCTGGATCCTGCTCATC
ATCGCCTCTGTGATTGGGATCATTGTCTACAGGCTGTCCGTGTTCATCGTGTTCTCCACGACGCTCCCCA
AGAACCCCAATGGGACAGACCCAATCCAGAAGTACCTGACCCCACAGATGGCCACGTCCATCACGGCTTC
CATTATTAGTTTCATCATCATCATGATCCTCAACACGATCTACGAGAAGGTGGCCATCATGATCACCAAC
TTTGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTGACCATGAAGATGTTCTTGTTCCAGTTTG
TCAACTACTACTCCTCCTGCTTCTACATCGCATTCTTCAAGGGCAAATTTGTCGGCTATCCGGGGGACCC
GGTGTACTGGCTGGGCAAATACAGAAACGAAGAGTGTGACCCGGGGGGCTGCCTCCTTGAACTGACTACA
CAGCTGACGATCATCATGGGGGGAAAGGCGATCTGGAACAACATACAAGAAGTTCTGCTCCCGTGGGTTA
TGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATTA
CCATCTGCAGCCCATGGGCAGGCTGGGATTGTTCTATGAGTACCTTGAAATGATTATTCAGTTTGGGTTC
GTCACCTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCCCTGGTGAACAATATACTGGAGATAA
GAGTGGATGCGTGGAAGCTTACAACACAGTTTCGACGCATGGTGCCAGAGAAAGCCCAGGATATCGGCGC
GTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACGCCATGATCATTGCCTTCACC
TCAGACATGATCCCCCGCCTGGTGTACTATTGGTCCTTCTCCATCCCTCCCTATGGGGACCACACTTACT
ACACCATGGACGGCTACATCAACAACACTCTCTCGGTCTTCAACATCACCGACTTCAAAAATACAGACAA
AGAAAACCCGTACATTGGGCTTGTCAGGCTCTCGTTCACAGTGTTAAACCAAAAGATTTGTCTCCTAACG
TGCCTGGTTGTACTTCCAGGTACCGTGACTTCCGAAACCCACCCGGGCACCCACAAGAGTATAAACACAA
CATCTACTATTGGCACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR212483 protein sequence
Red=Cloning site Green=Tags(s)

MQMMTRNVLLNMELEEDDDEDGDIGDVPDSRRPFLAPHTPLPSGLVLENFDQTIVCSTFGSLESQEDFRT
PEFEEFNGKPDSLFFTDGQRRIDFVLVYEDESKKENNKKGTNEKQKRKRQAYESNLISHGLQLEATRSVS
DDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKTRSTFDTFNWFTKVLRVNESVIKPEQEFFTAPFEK
SRMNDFYILDRDSFFNPATRSRIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCKFNYKSED
PNCPSERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLNQD
NCTWSKEVCDPDIGGQILMCPQCDRLCPFWRLNITCESSKEEERIPFTTCGKCIRITLCASAVFFWILLI
IASVIGIIVYRLSVFIVFSTTLPKNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITN
FELPRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVGYPGDPVYWLGKYRNEECDPGGCLLELTT
QLTIIMGGKAIWNNIQEVLLPWVMNLIGRYKRVSGSEKITPRWEQDYHLQPMGRLGLFYEYLEMIIQFGF
VTLFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFT
SDMIPRLVYYWSFSIPPYGDHTYYTMDGYINNTLSVFNITDFKNTDKENPYIGLVRLSFTVLNQKICLLT
CLVVLPGTVTSETHPGTHKSINTTSTIGT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001108108
ORF Size 2397 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001108108.1, NP_001101578.1
RefSeq Size 5451 bp
RefSeq ORF 2400 bp
Locus ID 315272
Cytogenetics 7q35
MW 92.2 kDa

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