Cnr1 (NM_012784) Rat Tagged ORF Clone

CAT#: RR212368

Cnr1 (Myc-DDK-tagged ORF) - Rat cannabinoid receptor 1 (brain) (Cnr1), (10 ug)

Product Images

Circular map for RR212368

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnr1
• Synonyms: SKR6R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212368 representing NM_012784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCGATCCTAGATGGCCTTGCAGACACCACCTTCCGTACCATCACCACAGACCTCCTCTACGTGG
  GCTCGAATGACATTCAGTATGAAGATATCAAAGGAGACATGGCATCCAAATTAGGATACTTCCCACAGAA
  ATTCCCTCTAACTTCCTTCAGGGGTAGTCCCTTCCAAGAAAAGATGACCGCAGGAGACAACTCCCCGTTG
  GTCCCAGCAGGAGACACAACAAACATTACAGAGTTCTATAACAAGTCTCTCTCGTCGTTCAAGGAGAATG
  AGGAGAACATCCAGTGTGGGGAGAACTTTATGGACATGGAGTGCTTTATGATTCTGAATCCCAGCCAGCA
  GCTGGCCATCGCTGTACTGTCCCTCACACTGGGCACCTTCACGGTTCTGGAGAACCTACTGGTGCTGTGT
  GTCATCCTGCACTCCCGCAGTCTCCGATGCAGGCCTTCCTACCACTTCATCGGCAGCCTGGCAGTGGCCG
  ACCTCCTGGGAAGTGTCATTTTTGTGTACAGCTTTGTTGACTTCCATGTATTCCACCGTAAAGACAGCCC
  CAATGTGTTTCTGTTCAAACTGGGTGGGGTTACAGCCTCCTTCACAGCTTCTGTGGGCAGCCTGTTCCTC
  ACAGCCATCGACAGGTACATATCCATTCACAGGCCTCTGGCCTATAAGAGGATCGTCACCAGGCCCAAGG
  CCGTTGTGGCCTTTTGCCTGATGTGGACTATCGCAATAGTAATCGCTGTGTTGCCTCTCCTGGGCTGGAA
  CTGCAAGAAGCTGCAATCTGTTTGCTCGGACATTTTCCCACTCATTGACGAGACCTACCTGATGTTCTGG
  ATTGGGGTGACCAGTGTGCTGCTGCTGTTCATTGTGTACGCGTACATGTACATTCTCTGGAAGGCTCACA
  GCCACGCGGTCCGCATGATTCAGCGTGGGACCCAGAAGAGCATCATCATCCACACGTCAGAAGACGGCAA
  GGTGCAGGTGACCCGGCCTGACCAAGCCCGCATGGACATTAGGCTGGCCAAAACCCTGGTTCTGATCCTG
  GTGGTGTTGATCATCTGCTGGGGCCCTCTGCTTGCGATCATGGTGTATGACGTCTTCGGGAAGATGAACA
  AGCTTATCAAGACGGTGTTTGCCTTCTGCAGTATGCTCTGCCTGCTGAACTCCACCGTGAACCCCATCAT
  CTATGCTCTGAGGAGCAAGGACCTGAGACATGCTTTCCGAAGCATGTTCCCTTCGTGCGAAGGCACCGCA
  CAGCCTCTAGACAACAGCATGGGGGACTCAGACTGCCTGCACAAGCACGCCAACAACACAGCCAGCATGC
  ACAGGGCCGCGGAGAGCTGCATCAAGAGCACCGTTAAGATCGCGAAGGTGACCATGTCTGTGTCCACAGA
  CACGTCCGCCGAGGCTCTG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212368 representing NM_012784
  Red=Cloning site Green=Tags(s)
  
  MKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNSPL
  VPAGDTTNITEFYNKSLSSFKENEENIQCGENFMDMECFMILNPSQQLAIAVLSLTLGTFTVLENLLVLC
  VILHSRSLRCRPSYHFIGSLAVADLLGSVIFVYSFVDFHVFHRKDSPNVFLFKLGGVTASFTASVGSLFL
  TAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCKKLQSVCSDIFPLIDETYLMFW
  IGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLIL
  VVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTA
  QPLDNSMGDSDCLHKHANNTASMHRAAESCIKSTVKIAKVTMSVSTDTSAEAL
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012784
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012784.4, NP_036916.1
• RefSeq Size: 5465 bp
• RefSeq ORF: 1422 bp
• Locus ID: 25248
• UniProt ID: P20272
• Cytogenetics: 5q21
• MW: 52.8 kDa
• Gene Summary: G-protein coupled receptor for psychoactive cannabinoids; may mediate inhibition of NO production and participate in retrograde signaling by endocannabinoids [RGD, Feb 2006]