Ezr (NM_019357) Rat Tagged ORF Clone

CAT#: RR212289

Ezr (Myc-DDK-tagged ORF) - Rat ezrin (Ezr), (10 ug)

Product Images

Circular map for RR212289

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ezr
• Synonyms: Vil2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212289 representing NM_019357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAGCCAATCAACGTCCGCGTGACCACCATGGATGCAGAGCTGGAGTTTGCCATCCAGCCAAACA
  CCACCGGGAAGCAGCTCTTTGATCAGGTGGTAAAGACCATTGGCCTCCGGGAAGTGTGGTACTTCGGCCT
  CCAGTATGTAGACAATAAAGGATTTCCTACCTGGCTGAAACTTGATAAAAAGGTCTCTGCACAGGAGGTT
  CGAAAGGAGAACCCTGTCCAGTTCAAATTCCGGGCCAAGTTCTACCCCGAGGATGTGGCTGATGAGCTCA
  TCCAGGACATCACACAGAAGCTCTTCTTCCTGCAAGTCAAGGAAGGGATCCTCAGCGATGAGATCTACTG
  CCCCCCAGAGACAGCCGTGCTCCTGGGGTCCTATGCCGTTCAGGCCAAGTTCGGAGATTATAACAAGGAA
  ATGCACAAGTCTGGGTACCTCAGCTCCGAGAGACTTATCCCTCAGAGAGTCATGGACCAGCACAAGCTCA
  GCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCACGCAGAACACCGAGGGATGCTCAAGGACAGTGC
  TATGCTCGAATACCTGAAGATTGCCCAGGACCTGGAAATGTATGGGATCAACTATTTCGAGATCAAAAAC
  AAGAAAGGAACAGATCTTTGGCTTGGAGTGGATGCCCTTGGACTTAACATTTATGAGAAAGATGACAAGT
  TGACCCCGAAGATCGGCTTCCCTTGGAGTGAAATCAGGAACATCTCTTTCAACGACAAGAAGTTTGTCAT
  CAAGCCCATCGACAAGAAGGCTCCTGACTTCGTGTTCTACGCTCCCCGCCTGAGAATTAACAAGCGGATT
  CTGCAGCTCTGCATGGGGAACCACGAGCTGTACATGCGCCGCAGGAAGCCCGACACCATCGAGGTGCAGC
  AGATGAAGGCCCAGGCTCGGGAGGAGAAGCACCAGAAGCAGCTGGAGCGACAACAGTTGGAAACCGAGAA
  GAAGAGGAGAGAAACTGTAGAGAGAGAAAAGGAACAGATGCTCCGGGAGAAGGAAGAGCTGATGCTTCGG
  CTGCAGGACTTTGAGCAGAAGACCAAGAGGGCAGAGAAAGAGCTCTCCGAGCAGATTGAGAAGGCCCTCC
  AGCTGGAGGAAGAGAGGAGGCGAGCCCAGGAGGAGGCTGAACGTCTGGAGGCCGACCGCATGGCCGCCCT
  GCGGGCCAAGGAAGAACTGGAGAGACAGGCACAGGATCAGATAAAGAGCCAGGAGCAGCTGGCTGCAGAG
  CTGGCAGAGTATACTGCCAAGATTGCCCTGCTGGAGGAGGCACGGAGGCGCAAGGAGGACGAGGTGGAGG
  AGTGGCAGCACCGGGCTAAAGAGGCCCAGGACGACCTGGTGAAGACCAAAGAGGAGCTGCACCTGGTGAT
  GACGGCTCCCCCGCCCCCACCCCCGCCCGTGTACGAGCCCGTGAATTACCACGTGCAGGAGGGGCTGCAG
  GATGAGGGAGCAGAGCCCATGGGCTACAGTGCCGAGCTCTCCAGCGAGGGCATCCTGGACGACCGTAACG
  AGGAGAAGCGGATCACCGAGGCAGAGAAGAACGAGCGCGTGCAGCGGCAGCTGCTGACTCTGAGCAATGA
  GTTGTCCCAGGCCCGGGATGAGAATAAGAGGACCCACAATGACATCATCCACAATGAGAACATGCGGCAA
  GGCCGGGACAAGTATAAGACGCTGCGGCAGATCAGGCAGGGCAACACCAAGCAGCGCATCGACGAGTTTG
  AGGCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212289 representing NM_019357
  Red=Cloning site Green=Tags(s)
  
  MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEV
  RKENPVQFKFRAKFYPEDVADELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKE
  MHKSGYLSSERLIPQRVMDQHKLSRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKN
  KKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRI
  LQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMLREKEELMLR
  LQDFEQKTKRAEKELSEQIEKALQLEEERRRAQEEAERLEADRMAALRAKEELERQAQDQIKSQEQLAAE
  LAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVNYHVQEGLQ
  DEGAEPMGYSAELSSEGILDDRNEEKRITEAEKNERVQRQLLTLSNELSQARDENKRTHNDIIHNENMRQ
  GRDKYKTLRQIRQGNTKQRIDEFEAM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019357
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019357.1, NP_062230.1
• RefSeq Size: 2973 bp
• RefSeq ORF: 1761 bp
• Locus ID: 54319
• UniProt ID: P31977
• Cytogenetics: 1q11
• MW: 69.4 kDa
• Gene Summary: connects the microvillar cytoskeleton to the plasma membrane [RGD, Feb 2006]