Hadh (NM_057186) Rat Tagged ORF Clone

CAT#: RR212204

Hadh (Myc-DDK-tagged ORF) - Rat hydroxyacyl-Coenzyme A dehydrogenase (Hadh), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR212204

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hadh
• Synonyms: Hadhsc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212204 representing NM_057186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCGTGACCAGGCAATTCGTGCGCTCCATGTCCTCCTCTTCCTCTGCGTCGGCGGCCGCCAAGA
  AGATCCTGATCAAGCATGTGACGGTCATCGGCGGCGGGCTGATGGGCGCCGGCATCGCGCAGGTAGCTGC
  AGCAACTGGCCATACAGTAGTGTTGGTGGACCAAACAGAGGACATCCTGGCAAAATCCAAGAAGGGAATT
  GAAGAGAGCCTTAAGAGAATGGCAAAGAAGAAGTTCACAGAAAACCCTAAGGCTGCCGATGAGTTTGTGG
  AGAAAACCCTGAGCTCCCTTTCAACCAGCACCGATGCCGCGTCCGTGGTGCACAGCACAGACCTGGTGGT
  GGAGGCCATTGTGGAGAACCTGAAGTTGAAGAATGAGCTATTCCAGAGGCTGGACAAGTTTGCTGCAGAA
  CACACCATCTTTGCCAGCAACACGTCTTCTTTGCAGATCACAAACATAGCCAATGCCACCACCAGACAAG
  ACCGATTTGCTGGCCTCCACTTTTTTAACCCCGTCCCCATGATGAAGCTTGTGGAGGTCATTAAAACACC
  GATGACCAGCCAGAAGACATTTGAATCTCTGGTTGACTTTTGTAAAACCTTAGGGAAGCATCCTGTTTCC
  TGCAAGGACACTCCTGGATTTATCGTGAACCGTCTCTTGGTGCCATACCTCATAGAAGCTATCCGGCTGC
  ATGAGCGAGGCGATGCATCTAAGGAAGACATCGACACCGCAATGAAGCTGGGAGCCGGGTATCCCATGGG
  TCCCTTTGAGCTTCTTGACTATGTTGGACTGGATACTACAAAGTTCATCTTAGACGGGTGGCACGAAATG
  GATCCGGAGAACCCCTTATTTCAGCCCAGTCCTTCCATGAATAATTTGGTGGCCCAGAAGAAGCTGGGCA
  AAAAGACCGGAGAAGGATTTTACAAATACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212204 representing NM_057186
  Red=Cloning site Green=Tags(s)
  
  MAFVTRQFVRSMSSSSSASAAAKKILIKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGI
  EESLKRMAKKKFTENPKAADEFVEKTLSSLSTSTDAASVVHSTDLVVEAIVENLKLKNELFQRLDKFAAE
  HTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFESLVDFCKTLGKHPVS
  CKDTPGFIVNRLLVPYLIEAIRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEM
  DPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057186
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057186.2, NP_476534.1
• RefSeq Size: 1659 bp
• RefSeq ORF: 945 bp
• Locus ID: 113965
• UniProt ID: Q9WVK7
• Cytogenetics: 2q43
• MW: 34.4 kDa
• Gene Summary: catalyzes the dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs [RGD, Feb 2006]