Gss (NM_012962) Rat Tagged ORF Clone

CAT#: RR212142

Gss (Myc-DDK-tagged ORF) - Rat glutathione synthetase (Gss), (10 ug)

Product Images

Circular map for RR212142

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gss
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212142 representing NM_012962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCAGCTGGGGAAGCATCTTGCAGGATGAGAAGCAGCTGGAAGAGTTGGCACAGCAGGCCATAG
  ACCGGGCCCTGGCTGAGGGGGTGTTGCTGAGGTCCGCAAAGAACCCCAGCTCCTCTGACGTGGTGACGTA
  TGCCCCATTCACGCTCTTCCCCTCACCAGTGCCCAGCACTCTGCTGGAGCAGGCCTATGCTGTGCAGATG
  GACTTCAACATCCTGGTAGATGCTGTCAGCCAGAACTCCGCCTTCCTGGAGCAAACACTGTCTAGCACCA
  TCAAAAAGGATGAGTATACTGCCCGTCTCTTTGATATCTACAAGCAAGTCCTGAAAGAGGGCATAGCCCA
  GACTGTGTTCCTGGGCCTCAATCGTTCAGATTACATGTTCCAGTGCAGCGCAGACGGCTCCAAAGCCCTG
  AAACAGATTGAGATCAACACTATCTCTGCCAGCTTTGGGGGCCTGGCCTCCCGGACTCCGGCTGTGCACC
  GACATGTTCTCAATGTCCTGAATAAGACCAACGAAGCTTCCAAGATCCTGTCCAACAACCCCAGCAAGGG
  ACTGGCCCTGGGGATCGCCAAAGCCTGGGAGCTCTATGGCTCAGCCAATGCCGTGGTGCTACTGATTGCT
  CAGGAGAAGGAAAGGAACATATTTGACCAGCGTGCCATAGAGAACGAGCTGCTAGACAGGAAGATCCATG
  TAATCCGCCGAAGATTTGAAGATGTCTCTGAAAGGGGTTCTCTAGACCAAAACCGAAGGCTGTTTATGGA
  GGACCAGGAAGTTGCTGTGGTTTACTTCCGAGATGGCTACATGCCCAGTCAGTATAACGCACAGAACTGG
  GAAGCTCGCCTGCTGCTAGAGAGATCATGTGCTGCCAAGTGTCCCGACATTGCCACACAGCTGGCTGGCA
  CTAAGAAGGTGCAGCAGGAACTGAGCAGGGTGGGCCTGCTGGAAGCGCTGCTCCCGGGCCAGCCCGAGGC
  TGTGGCCCGCCTCCGTGCCACCTTTGCTGGCCTCTATTCACTGGACATGGGTGAAGAAGGGGACCAGGCT
  GTCGCTGAGGCCCTTGCTGCCCCTAGCCACTTTGTGCTGAAGCCCCAAAGAGAGGGCGGAGGTAATAACT
  TCTATGGGGAGGAAATGGTACACGCTCTGGAGCAGCTGAAAGACAGCGAGGAGAGAGCCTCCTACATCCT
  CATGGAGAAGATTGAACCTGAGCCTTTTAGGAATTGCTTACTACGGCCTGGCAGCCCTGCCCAAGTGGTC
  CAGTGCATCTCGGAGCTGGGTATTTTTGGAGTCTATGTCAGACAGGGAACAACACTTGTGATGAACAAGC
  ATGTGGGGCATCTGCTTCGAACCAAAGCCATTGAACATGCAGATGGAGGTGTGGCAGCAGGAGTGGCAGT
  CCTGGACAACCCCTACCCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212142 representing NM_012962
  Red=Cloning site Green=Tags(s)
  
  MATSWGSILQDEKQLEELAQQAIDRALAEGVLLRSAKNPSSSDVVTYAPFTLFPSPVPSTLLEQAYAVQM
  DFNILVDAVSQNSAFLEQTLSSTIKKDEYTARLFDIYKQVLKEGIAQTVFLGLNRSDYMFQCSADGSKAL
  KQIEINTISASFGGLASRTPAVHRHVLNVLNKTNEASKILSNNPSKGLALGIAKAWELYGSANAVVLLIA
  QEKERNIFDQRAIENELLDRKIHVIRRRFEDVSERGSLDQNRRLFMEDQEVAVVYFRDGYMPSQYNAQNW
  EARLLLERSCAAKCPDIATQLAGTKKVQQELSRVGLLEALLPGQPEAVARLRATFAGLYSLDMGEEGDQA
  VAEALAAPSHFVLKPQREGGGNNFYGEEMVHALEQLKDSEERASYILMEKIEPEPFRNCLLRPGSPAQVV
  QCISELGIFGVYVRQGTTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012962
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012962.1, NP_037094.1
• RefSeq Size: 1882 bp
• RefSeq ORF: 1425 bp
• Locus ID: 25458
• UniProt ID: P46413
• Cytogenetics: 3q42
• MW: 52.3 kDa
• Gene Summary: catalyzes the synthesis of glutathione from gamma-L-glutamyl-L-cysteine and glycine; activity is ATP-dependent [RGD, Feb 2006]