Rbms1 (NM_001012184) Rat Tagged ORF Clone

CAT#: RR212095

Rbms1 (Myc-DDK-tagged ORF) - Rat RNA binding motif, single stranded interacting protein 1 (Rbms1), (10 ug)

Product Images

Circular map for RR212095

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbms1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212095 representing NM_001012184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAAGTGTGGAAACAACAGATGTACCCTCAGTACGCCACCTATTACTACCCCCAGTATCTGCAAG
  CCAAGCAGTCTCTGGTCCCAGCGCACCCCATGGCCCCTCCCAGTCCCAGCACCACCAGCAGTAATAACAA
  CAGTAGCAGCAGTAGCAACTCAGGATGGGATCAGCTGAGTAAGACGAACCTCTACATCCGAGGCCTGCCC
  CCCAACACCACTGATCAAGACCTGGTGAAGCTCTGTCAACCATACGGGAAGATTGTCTCCACGAAGGCGA
  TTTTGGATAAGGCAACCAACAAGTGCAAAGGTTATGGATTTGTTGACTTTGACAGTCCTGCAGCAGCCCA
  GAAAGCTGTCTCCGCCCTGAAGGCGAGTGGAGTCCAGGCTCAGATGGCAAAGCAACAGGAACAAGATCCT
  ACCAACCTGTACATTTCTAACCTGCCACTGTCCATGGATGAACAAGAACTTGAAAATATGCTCAAACCCT
  TTGGACAAGTTATTTCTACAAGGGTCCTGCGTGATTCCAGTGGTACCAGCCGTGGTGTTGGCTTTGCCAG
  GATGGAATCAACGGAAAAATGCGAAGCTGTAATTGGTCATTTTAATGGAAAATTCATCAAGACCCCACCA
  GGAGTTTCTGCTCCTACAGAACCTTTACTGTGCAAGTTTGCGGATGGAGGACAGAAGAAGAGACAGAACC
  CAAACAAGTACATCCCTAATGGGCGGCCATGGCCCAGAGAAGGAGAGGCCGGAATGACACTCACTTACGA
  CCCGACTACAGCTGCTCTACACAACGGATTTTATCCTTCACCATACAGTATTGCCACAAACCGAATGATC
  ACTCAAACTTCTCTTACACCCTATATTGCATCTCCTGTATCTGCCTACCAGGTGCAGAGTCCTTCCTGGA
  TGCAGCCTCAACCATACATTCTGCAGCATCCTGGTGCCGTGTTAACTCCCTCCATGGAACACACCATGTC
  ACTACAACCTGCTTCTATGATCAGCCCTCTGGCCCAGCAGATGAGTCACCTGTCACTAGGCAGCACCGGA
  ACATACATGCCTGCAACGTCAGCCATGCAAGGAGCCTACTTACCACAGTACACACACATGCAGACTGCGA
  CGGTTCCCGTTGAGGAAGCAAGTGGCCAGCAGCAGGTGACTGTGGAGACGTCTAATGACCATTCTCCATA
  TACCTTTCCACCCAATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212095 representing NM_001012184
  Red=Cloning site Green=Tags(s)
  
  MGKVWKQQMYPQYATYYYPQYLQAKQSLVPAHPMAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLP
  PNTTDQDLVKLCQPYGKIVSTKAILDKATNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDP
  TNLYISNLPLSMDEQELENMLKPFGQVISTRVLRDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPP
  GVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRPWPREGEAGMTLTYDPTTAALHNGFYPSPYSIATNRMI
  TQTSLTPYIASPVSAYQVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLGSTG
  TYMPATSAMQGAYLPQYTHMQTATVPVEEASGQQQVTVETSNDHSPYTFPPNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012184
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012184.1, NP_001012184.1
• RefSeq Size: 1979 bp
• RefSeq ORF: 1212 bp
• Locus ID: 362138
• UniProt ID: Q5PQP1
• Cytogenetics: 3q21
• MW: 44.1 kDa
• Gene Summary: Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication (By similarity).[UniProtKB/Swiss-Prot Function]