Disc1 (NM_175596) Rat Tagged ORF Clone

CAT#: RR212017

Disc1 (Myc-DDK-tagged ORF) - Rat disrupted in schizophrenia 1 (Disc1), (10 ug)

Product Images

Circular map for RR212017

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Disc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212017 representing NM_175596
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCGCGGGTTCCCGGGGCGCTTGGATCCACAGTCCGAGCCACTGCCCAGGCAATGGGCATGGCT
  CGCCGCCTGCAGTAGCCCCTCAGAGGCGGCGGCTGACACGGAGACCAGGCTACATGAGAAGCACAGCCAG
  TCCTGGGATCGGGTTCCTGTCTCCAGCAGTGGGCATGCCACGTCCGATCTCAGCGGGGCTGACAGGCCAG
  GAGTTCTACCCCTCACAGTCCAAGGCTAGACAGTGCAGCCTTGACCTCAGAAGCCACTGCCAAGACTCAT
  TGGTGGGCAACCCTTTTCTCAAGGGCTCCCTTGGCCCTGCTGTGACCTCTGTGGGCCACCTGCACCCAGC
  CCAGGGCTCTATGAGAGAAAGGATGGTGCACTCTGGGGTTCATTCCGGGAATGACAGAAGACAATCCGAG
  AGGCTGACTGGGGACAGTGGTTGTCGGCAAGAATTTTTATCTTCAGACAGCTCTAAGTCTCTGGCTTCTA
  GCCTTGATGTAGCTTGGAGCAAGGGATCGAGGGGCCTGAAGACTGTGAGGCCTCTGGTATCACCGGCGTC
  GAATGGCCCCGTTGACATCCCATCCCTTCCTGGGTTCCAAGACACCTTTACTTCCAACTTCAGCTTCATC
  CGACTCTCCCTCGGTGCTGCTGGAGAACGCGGAGAAGCAGAAGGTTGCCTGCCATCCAGAGAGGCCGAAC
  CCCTGCATCAGAGCCCCCAAGAGATGGCAGCTGAAGGATCCGGCTCAGACAGGCCCCACGGGGAGCCTCG
  GCATCTCTGGACCTTCAGTCTTCACGCTGCTCCGGGCTTGGTGGACTTGGCTCAGGGGACGAGGAGCAAC
  AGGCAACCAGAATGTGGCATGGTCTCCTCCTCCGATGCTGGCTTCTCTTCCCAGGATGCATCCCCCGCTG
  GAGGGCGGAGCGACCAGGACGGAGGCTGGGCGGATGCCCATGGATGGCACGCTCTGCTCAGGGAATGGGA
  GCCTATGCTGCAGGACTACCTACTGAGCAACCGCAGGCAGCTGGAGGTCACTTCCTTGATTTTAAAGCTT
  CAGAAACTTCAAGAAAAAGCGGTCGAGGATGGCGATTACGACATGGCAGAGACACTGAGACAGAGATTGG
  AAGACCTGGAACAGGAGAAAGGCCGCCTGCCCTGGGCTCTGCCCTCACAGCAACCAGCCCTTCGCAGCTT
  CTTGGGGTACCTGGCAACACAGACACACGCGGCCTTGCATGGAGCCCCCCAAAGGGCTGGCAGCGATGAC
  CCAGAAGCCCCACTTGAGGGACAGCGGAGGACCACCGCCCAGGATAGCCTGCCGGGACTAGCCGTCACCA
  GGCGGGACTGGCTGATGCGAGAGAAAGAGCAATTGCAGAAGGAAATCGAAGCTCTCCGGGCAAGGGTGTC
  TGTGCTGGAGGCGAAGGAACAACGGCTGAGCCAAGAGTTGGAGGATCAGGAGATGCTGCTCCGGTGGCAG
  GGCTGTGACCAGATGGCACTGGTGGCACAGCTGTCCCCAGGCCAGCTGCAGGAGGTCAGCAAGGCCTTGG
  GAGAGACTCTGACCTCTGCCAGATGGGCTCCCTTCCGCGTGGAGCCACCCGAAACCCTCAGGAGCCTCCG
  GGAAAGGACAAAGTCACTCGACCTGGCTGTTAGAGAACTCACTGAGCAGGTATGCTCAGGTGAGAAGCTA
  TGCAGCTCTCTGAGGAAGAGACTCGCTGACCTTGACACCAGGCTGCCTGCCTTGCTGGAAGCCAAGATGC
  TGGCCCTATCAGGAAGCTGCTTCTCTACAGCCAAGGAGCTCGCAGAGGAGATTTGGGCCGTGTCATCGGA
  GCGGGAAGGGCTAGAGATGTTCCTGGGCAGGCTGCTGGCCCTCAGCTCCAGGAACACGAGAAGACTGGGC
  AGCGTCAAAGAGGACTACCTCAGGTGCAGGCAGGACCTGGCCCTCCAGGAAGCCGCCCACAAAACACGCG
  TGAAGGCAAACACTGTGAAGTGCACGGAAGTGTTGGAGGGTCAGCTGAGCTGCTGCAGGTGCCCGCTGCT
  TGAGAGAGTGTGGAAAGCGGACTTGGAGGCGTGTCAGTTGCTGATGCAGAGCCTAGAGATCCAGGAAGCA
  GGCAGCAGCTCACATGTGGAGGATGAGAAGCAGGTGCACAGCACAGGAGAGGCGGCTCAGACAGCTGCCC
  TGGCTGTCCCTAGAACACCCCACCCCGAAGAGGAAAAGTCCCCCTTGCAGGAATCTCATGTCGTTTTTGC
  TGAAGTTGGAGACAAATGCGAAGCCATAGGCATGAGGCTCCTGCACCTGGAAGACCAGCTTCTCGGCGCC
  ATGCACGGTCACGATGAAGCTCTCTTTCAGTCTCTCCAGGGGGAGCTCCAGATGGTTAAGGAAACACTAC
  AGACCATGTTCCTGCAGCTCCAGCCAGCAAAGGAGGCAGGAGGAGAGGCCTCAGCTTCCTATTCGACAGC
  TGGTGCTCAGGAAGCCGAGGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212017 representing NM_175596
  Red=Cloning site Green=Tags(s)
  
  MQGAGSRGAWIHSPSHCPGNGHGSPPAVAPQRRRLTRRPGYMRSTASPGIGFLSPAVGMPRPISAGLTGQ
  EFYPSQSKARQCSLDLRSHCQDSLVGNPFLKGSLGPAVTSVGHLHPAQGSMRERMVHSGVHSGNDRRQSE
  RLTGDSGCRQEFLSSDSSKSLASSLDVAWSKGSRGLKTVRPLVSPASNGPVDIPSLPGFQDTFTSNFSFI
  RLSLGAAGERGEAEGCLPSREAEPLHQSPQEMAAEGSGSDRPHGEPRHLWTFSLHAAPGLVDLAQGTRSN
  RQPECGMVSSSDAGFSSQDASPAGGRSDQDGGWADAHGWHALLREWEPMLQDYLLSNRRQLEVTSLILKL
  QKLQEKAVEDGDYDMAETLRQRLEDLEQEKGRLPWALPSQQPALRSFLGYLATQTHAALHGAPQRAGSDD
  PEAPLEGQRRTTAQDSLPGLAVTRRDWLMREKEQLQKEIEALRARVSVLEAKEQRLSQELEDQEMLLRWQ
  GCDQMALVAQLSPGQLQEVSKALGETLTSARWAPFRVEPPETLRSLRERTKSLDLAVRELTEQVCSGEKL
  CSSLRKRLADLDTRLPALLEAKMLALSGSCFSTAKELAEEIWAVSSEREGLEMFLGRLLALSSRNTRRLG
  SVKEDYLRCRQDLALQEAAHKTRVKANTVKCTEVLEGQLSCCRCPLLERVWKADLEACQLLMQSLEIQEA
  GSSSHVEDEKQVHSTGEAAQTAALAVPRTPHPEEEKSPLQESHVVFAEVGDKCEAIGMRLLHLEDQLLGA
  MHGHDEALFQSLQGELQMVKETLQTMFLQLQPAKEAGGEASASYSTAGAQEAED
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175596
• ORF Size: 2472 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175596.2, NP_783186.2
• RefSeq Size: 2475 bp
• RefSeq ORF: 2475 bp
• Locus ID: 307940
• UniProt ID: Q810H6
• Cytogenetics: 19q12
• MW: 90.1 kDa
• Gene Summary: interacts with a variety of cytoskeletal proteins; truncated mutant form is associated with Schizophrenia [RGD, Feb 2006]