Pak1 (NM_017198) Rat Tagged ORF Clone

CAT#: RR211616

Pak1 (Myc-DDK-tagged ORF) - Rat p21 (CDKN1A)-activated kinase 1 (Pak1), (10 ug)

Product Images

Circular map for RR211616

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pak1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211616 representing NM_017198
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATAACGGCTTAGACGTCCAGGACAAACCCCCAGCCCCTCCGATGAGAAACACCAGCACTATGA
  TTGGAGCTGGCAGCAAAGACCCTGGAACCCTAAACCACGGTTCCAAACCTCTGCCTCCAAACCCAGAGGA
  GAAGAAAAAGAAGGACCGGTTCTATCGATCCATCTTAGCTGGAGATAAAACAAATAAAAAGAAGGAGAAG
  GAGCGGCCAGAGATTTCTCTTCCTTCAGATTTTGAGCATACAATTCATGTTGGTTTTGATGCTGTCACAG
  GGGAGTTTACGGGGATGCCAGAACAGTGGGCCCGCTTGCTTCAAACATCAAATATCACCAAGTCAGAGCA
  GAAGAAAAACCCACAGGCTGTTCTGGATGTGTTGGAATTTTATAACTCCAAGAAGACCTCCAATAGTCAG
  AAGTACATGAGTTTTACAGATAAGTCAGCTGAAGATTATAATTCTTCTAATACTTTGAATGTGAAGACTG
  TGTCTGAGACCCCAGCAGTGCCACCAGTGTCAGAAGATGAAGATGATGATGACGATGCTACCCCACCTCC
  AGTGATTGCTCCACGCCCAGAACACACAAAATCTGTATATACACGGTCTGTGATTGAACCACTTCCTGTT
  ACTCCAACTCGGGATGTGGCTACATCTCCTATTTCTCCTACTGAGAATAACACCACTCCGCCAGATGCTT
  TGACCCGGAATACTGAAAAGCAGAAGAAGAAGCCTAAAATGTCTGATGAGGAGATCTTAGAGAAATTACG
  GAGCATAGTGAGTGTGGGCGATCCTAAGAAGAAGTACACACGCTTCGAGAAGATTGGACAAGGTGCTTCA
  GGCACAGTGTACACTGCAATGGATGTAGCCACAGGGCAGGAGGTGGCCATTAAACAGATGAACCTTCAGC
  AGCAGCCGAAGAAAGAACTCATTATTAATGAGATCCTGGTCATGAGGGAAAACAAAAACCCAAACATTGT
  GAACTATCTGGACAGTTACCTTGTGGGAGATGAGCTATGGGTTGTCATGGAATACTTGGCCGGAGGCTCC
  TTAACAGATGTGGTGACAGAAACCTGTATGGATGAAGGCCAGATAGCAGCTGTGTGCCGAGAGTGTCTAC
  AAGCTTTGGAGTTCCTGCATTCAAACCAAGTCATTCACAGAGACATCAAGAGTGACAATATTCTGCTGGG
  AATGGATGGCTCTGTCAAATTAACTGACTTTGGATTCTGTGCACAGATAACTCCAGAGCAGAGCAAAAGG
  AGCACCATGGTGGGAACTCCATATTGGATGGCACCTGAAGTTGTGACACGCAAGGCCTATGGACCCAAGG
  TTGACATCTGGTCCCTGGGTATTATGGCAATTGAAATGATTGAGGGGGAGCCCCCATACCTCAATGAAAA
  CCCTTTGAGAGCCTTGTACCTCATTGCTACCAATGGGACGCCAGAGCTTCAGAACCCAGAGAAGTTGTCA
  GCTATTTTCCGGGACTTTTTGAACCGCTGTCTTGAGATGGATGTGGAGAAGAGAGGTTCAGCTAAAGAGC
  TACTGCAGCATCAATTCCTGAAGATTGCCAAGCCTCTCTCCAGCCTGACTCCACTGATTGCTGCAGCAAA
  AGAGGCAACCAAGAACAATCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211616 representing NM_017198
  Red=Cloning site Green=Tags(s)
  
  MSNNGLDVQDKPPAPPMRNTSTMIGAGSKDPGTLNHGSKPLPPNPEEKKKKDRFYRSILAGDKTNKKKEK
  ERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQ
  KYMSFTDKSAEDYNSSNTLNVKTVSETPAVPPVSEDEDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPV
  TPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGAS
  GTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGS
  LTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKR
  STMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS
  AIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017198
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017198.1, NP_058894.1
• RefSeq Size: 2539 bp
• RefSeq ORF: 1635 bp
• Locus ID: 29431
• UniProt ID: P35465
• Cytogenetics: 1q32
• MW: 60.6 kDa
• Gene Summary: serine/threonine protein kinase; binds and forms complex specifically with activated (GTP-bound) p21, leading to inhibiting p21 GTPase activity [RGD, Feb 2006]