Rara (NM_031528) Rat Tagged ORF Clone

CAT#: RR211595

Rara (Myc-DDK-tagged ORF) - Rat retinoic acid receptor, alpha (Rara), (10 ug)

Product Images

Circular map for RR211595

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rara
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211595 representing NM_031528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGAAAGTGTAGAAGTCGGGGGGCTTGCCCCCGCCCCTAACCCCTTCCTAGTGGTGGACTTTTATA
  ACCAGAACCGGGCCTGTTTGCTCCAGGAGAAGGGGCTCCCTGCCCCGGGTCCCTACTCCACCCCACTCCG
  GACTCCGCTTTGGAATGGCTCAAACCACTCCATCGAGACCCAGAGCAGCAGTTCGGAAGAGATAGTGCCC
  AGCCCTCCCTCACCACCACCCCTGCCTCGAATCTACAAGCCTTGCTTTGTTTGTCAAGACAAGTCATCCG
  GCTACCACTATGGGGTTAGCGCCTGTGAGGGCTGTAAGGGCTTCTTCCGACGCAGTATCCAAAAGAACAT
  GGTGTATACGTGTCACCGGGACAAGAACTGCATCATCAACAAGGTGACCCGGAACCGCTGCCAGTACTGC
  CGGCTGCAGAAATGCTTCGAAGTGGGCATGTCCAAGGAGTCGGTGCGAAACGACCGAAACAAAAAGAAGA
  AAGAAACACCCAAGCCCGAGTGCTCAGAGAGCTACACGCTGACACCGGAGGTGGGGGAACTCATCGAGAA
  GGTGCGCAAAGCACACCAGGAGACCTTCCCGGCCCTCTGCCAGCTGGGCAAGTACACTACGAACAACAGC
  TCAGAACAACGTGTCTCTCTGGACATTGACCTCTGGGACAAGTTCAGTGAACTCTCCACCAAGTGTATCA
  TTAAGACTGTGGAGTTCGCCAAGCAGCTTCCCGGCTTCACCACCCTCACCATTGCCGACCAGATTACCCT
  TCTCAAGGCTGCCTGCCTGGACATCCTGATTCTGCGAATCTGCACGCGGTACACACCTGAGCAAGACACA
  ATGACCTTCTCAGATGGACTGACCCTGAACCGGACTCAGATGCACAACGCTGGCTTTGGCCCCCTCACCG
  ACTTGGTCTTTGCCTTCGCCAACCAGCTGCTGCCCCTGGAGATGGACGATGCTGAGACCGGACTGCTCAG
  TGCCATCTGCCTCATCTGTGGAGACCGACAGGACCTGGAGCAGCCAGACAAGGTGGACATGCTGCAGGAG
  CCGCTGTTGGAAGCACTGAAAGTCTATGTCCGGAAACGGAGGCCCAGCCGACCCCACATGTTCCCCAAGA
  TGCTGATGAAGATCACGGACCTTCGGAGTATCAGCGCCAAGGGAGCTGAACGGGTGATCACATTGAAGAT
  GGAGATCCCTGGTTCCATGCCACCACTTATCCAGGAAATGTTGGAGAACTCTGAGGGCTTGGACACTCTA
  AGCGGACAGTCGGGGGGCGGAACACGAGATGGGGGTGGCCTGGCCCCTCCTCCGGGTAGCTGTAGCCCCA
  GCCTCAGTCCCAGCTCCCACAGAAGCAGCCCAGCCACTCAATCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211595 representing NM_031528
  Red=Cloning site Green=Tags(s)
  
  MYESVEVGGLAPAPNPFLVVDFYNQNRACLLQEKGLPAPGPYSTPLRTPLWNGSNHSIETQSSSSEEIVP
  SPPSPPPLPRIYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYC
  RLQKCFEVGMSKESVRNDRNKKKKETPKPECSESYTLTPEVGELIEKVRKAHQETFPALCQLGKYTTNNS
  SEQRVSLDIDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPEQDT
  MTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLICGDRQDLEQPDKVDMLQE
  PLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTL
  SGQSGGGTRDGGGLAPPPGSCSPSLSPSSHRSSPATQSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031528
• ORF Size: 1377 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031528.2, NP_113716.2
• RefSeq Size: 2138 bp
• RefSeq ORF: 1380 bp
• Locus ID: 24705
• Cytogenetics: 10q31
• MW: 50.9 kDa
• Gene Summary: mediates all-trans-retinoic acid induction of mitogen-activated protein kinase phosphatase 1 (MKP-1); contributes to regulation of oxidative stress-induced anti-apoptosis [RGD, Feb 2006]