Cacna1g (NM_031601) Rat Tagged ORF Clone

CAT#: RR211239

Cacna1g (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), (10 ug)

Product Images

Circular map for RR211239

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211239 representing NM_031601
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGTAGCTTCACGCAGCTCAACG
  ACCTGTCCGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCGTACCCGGCGCTAGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGTATGCTGGTCATTCTTCTCA
  ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAGCGCTGCCGGATCCT
  GCAGGCCTTCGATGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTGGCCTTGGGC
  ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATTGTCATTGCAGGGA
  TGCTGGAGTATTCGCTGGACCTGCAGAACGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGACACCTTGCCTATG
  CTGGGCAACGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTGGGCGTCCAGCTGTGGG
  CAGGACTGCTTCGCAACCGATGCTTCCTCCCCGAGAACTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCC
  TTATTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAATGGCATGAGA
  TCCTGCAGGAGTGTGCCCACACTGCGTGGGGAAGGCGGTGGTGGCCCACCCTGCAGTCTGGACTATGAGA
  CCTATAACAGTTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTATACCAACTGCTCTGCGGGCGA
  GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACACTGGAGGGCTGGGTCGACATCATGTACTTCGTAATGGACGCTCACTCCTTCTACAACTTCATCT
  ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA
  GTTCTCCGAGACCAAACAGCGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT
  AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTACTCAAGTACCTGGTGTACATCC
  TCCGAAAAGCAGCCCGAAGGCTGGCCCAGGTCTCTAGGGCTATAGGCGTGCGGGCTGGGCTGCTCAGCAG
  CCCAGTGGCCCGTAGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCACTCGCTCACACCGTCGTCTGTCT
  GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG
  TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCTCGCCGGCTCATGCTACCACCACC
  CTCTACACCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC
  TGCCACTTGGAGCCAGTCCGTTGCCAGGCACCCCCTCCCAGATGCCCATCGGAGGCATCTGGTAGGACTG
  TGGGTAGTGGGAAGGTGTACCCCACTGTGCATACCAGCCCTCCACCAGAGATACTGAAGGATAAAGCACT
  AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC
  TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT
  GCTCCAAGGCAGACAGTGGAGCCTGCGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGAGA
  GCCAGAGTCCGCTGACCATGTCATGCCTGACTCAGACAGCGAGGCTGTGTATGAGTTCACACAGGACGCT
  CAGCACAGTGACCTCCGGGATCCCCACAGCCGGCGGCGACAGCGGAGCCTGGGCCCAGATGCAGAGCCTA
  GTTCTGTGCTGGCTTTCTGGAGGCTGATCTGTGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGG
  CCGGGGAATCATGATCGCCATCCTGGTCAATACACTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAG
  GAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGA
  AACTGCTTGTCTACGGTCCCTTTGGCTACATTAAGAATCCCTACAACATCTTTGATGGTGTCATTGTGGT
  CATCAGTGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATG
  CGGGTGCTGAAGCTGGTGCGCTTCCTGCCGGCCCTGCAGCGCCAGCTCGTGGTGCTCATGAAGACCATGG
  ACAACGTGGCCACCTTCTGCATGCTCCTCATGCTGTTCATCTTCATCTTCAGCATCCTGGGCATGCATCT
  CTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTCGACTCCCTG
  CTCTGGGCCATCGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTCTACAACGGCA
  TGGCCTCCACATCGTCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTATGTGCTCTT
  TAACCTGCTGGTGGCCATTCTTGTGGAAGGCTTCCAGGCAGAGGGAGATGCCACCAAGTCTGAGTCAGAG
  CCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGAAAGAAGCGCTTGGCCCTGGTGGCTTTGG
  GAGAACACGCGGAACTACGAAAGAGCCTTTTGCCACCCCTCATCATCCATACGGCTGCGACACCAATGTC
  ACTACCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGACGTACCAGTAGCAGT
  GGGTCCGCTGAGCCTGGAGCTGCCCACCATGAGATGAAATCTCCGCCAAGTGCCCGCAGCTCCCCGCACA
  GTCCCTGGAGTGCGGCAAGCAGCTGGACCAGCAGGCGCTCCAGCAGGAACAGCCTGGGCCGGGCCCCCAG
  CCTAAAGCGGAGGAGCCCGAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGCCAGGAGAGTCAGGAT
  GAGGAGGAAAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGACCATCGCCACAGGGGTTCCTTGG
  AACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACTCTGCAGGTGCCGGGGCTGCACCGCACAGCCAG
  CGGCCGGAGCTCTGCCTCTGAGCACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACC
  CTGAGGACTGATGACCCCCAACTGGATGGGGATGATGACAATGATGAGGGAAATCTGAGCAAAGGGGAAC
  GCATACAAGCCTGGGTCAGATCCCGGCTTCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCGGCCTATAT
  CTTTCCTCCTCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTG
  GTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCG
  CTGAGCGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTTCTAGCTGAAATGACAGTGAA
  GGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAATGTGCTGGACGGC
  TTGCTGGTGCTCATCTCCGTCATCGACATCCTGGTCTCCATGGTCTCCGACAGCGGCACCAAGATCCTTG
  GCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTGCGTCCACTCAGGGTCATCAGCCGGGCCCAGGGACT
  GAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATTTGCTGTGCC
  TTCTTCATCATTTTTGGAATTCTCGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGG
  ACACCAGGAACATCACTAACAAATCCGACTGCGCTGAGGCCAGCTACCGATGGGTCCGGCACAAGTACAA
  CTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGTTGGGTTGACATC
  ATGTATGATGGGCTGGATGCTGTGGGTGTGGATCAGCAGCCCATCATGAACCACAACCCCTGGATGCTGC
  TATACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTTGTCCTGAACATGTTTGTGGGCGTGGTGGTGGA
  GAACTTCCATAAGTGCAGACAGCACCAGGAGGAGGAGGAGGCGAGGCGGCGTGAGGAGAAGCGACTACGG
  AGGCTGGAGAAAAAGAGAAGGAGTAAGGAGAAGCAGATGGCCGAAGCCCAGTGCAAGCCCTACTACTCTG
  ACTACTCGAGATTCCGGCTCCTTGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGG
  TGTCATCGGGCTGAACGTGGTCACTATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCT
  CTGAAGATCTGCAATTACATCTTTACCGTCATCTTTTGTCCTTTGAGTCAGTTTTCAAACTTGTGGGCCT
  TTGGCTTCCGCCGTTTCTTCCAGGACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCAT
  GGGCATCACACTGGAGGAGATTGAGGTCAATGCTTCGCTGCCCATCAACCCCACCATCATCCGTATCATG
  AGGGTGCTCCGCATTGCTCGAGTTCTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACA
  CGGTGATGCAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCTCTTCATGTTATTGTTTTTCATCTTTGC
  AGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGTCGG
  CATGCCACCTTTAGGAACTTTGGTATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGA
  ATGGTATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTACAACACTGTCATCTCCCC
  TATCTACTTTGTGTCCTTCGTGCTGACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCTGTGCTGATG
  AAGCACCTGGAAGAAAGCAACAAAGAGGCCAAGGAGGAGGCCGAGCTCGAGGCCGAGCTGGAGCTGGAGA
  TGAAGACGCTCAGCCCGCAGCCCCACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCCGGGGTGGAGGGTGT
  CAACAGTCCTGACAGCCCTAAGCCTGGGGCTCCACACACCACTGCCCACATTGGAGCAGCCTCGGGCTTC
  TCCCTTGAGCACCCCACGATGGTACCCCACCCCGAGGAGGTGCCAGTCCCCCTAGGACCAGACCTGCTGA
  CTGTGAGGAAGTCTGGTGTCAGCCGGACGCACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAG
  CACTGCTGAGAGATCCCTAGGACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCC
  GTTCACTCCCAACCAGCAGACACCAGCTGCATCCTACAGCTTCCCAAAGATGTGCACTATCTGCTCCAGC
  CTCATGGGGCCCCCACCTGGGGCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAG
  GCCTCTCAGGCGCCAGGCAGCAATAAGGACTGACTCCCTGGATGTGCAGGGCCTGGGTAGCCGGGAAGAC
  CTGTTGTCAGAGGTGAGTGGGCCCTCCTGCCCTCTGACCCGGTCCTCATCCTTCTGGGGCGGGTCGAGCA
  TCCAGGTGCAGCAGCGTTCCGGCATCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCC
  AGGCCTGGAACCCAGCTGGGCCAAGGACCCTCCAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTG
  AGCTGGATTTCAGGAGACCTCCTTCCCAGCAGCCAGGAAGAACCCCTGTCCCCACGGGACCTGAAGAAGT
  GCTACAGTGTAGAGACCCAGAGCTGCAGGCGCAGGCCTGGGTCCTGGCTAGATGAACAGCGGAGACACTC
  CATTGCTGTCAGCTGTCTGGACAGCGGCTCCCAACCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGC
  CAACCTCTTGGGGGTCCTGGGAGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCC
  CGGAGAGCCAGGGCTCTCGGCCCCCATGCAGTCCTGGTGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGA
  CTCTAAGGATCCCTCGGTCTCCAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGACACG
  CTGAGTCTCTCTGGTTTGTCTTCTGACCCAACAGACATGGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211239 representing NM_031601
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGEGGGGPPCSLDYETYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA
  STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAIGVRAGLLSSPVARSGQEPQPSGSCTRSHRRLS
  VHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRRLMLPPPSTPTPSGGPPRGAESVHSFYHAD
  CHLEPVRCQAPPPRCPSEASGRTVGSGKVYPTVHTSPPPEILKDKALVEVAPSPGPPTLTSFNIPPGPFS
  SMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHVMPDSDSEAVYEFTQDA
  QHSDLRDPHSRRRQRSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPE
  ELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLM
  RVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSL
  LWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESE
  PDFFSPSVDGDGDRKKRLALVALGEHAELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSS
  GSAEPGAAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQD
  EEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLART
  LRTDDPQLDGDDDNDEGNLSKGERIQAWVRSRLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHV
  VLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDG
  LLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCA
  FFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDI
  MYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLR
  RLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEA
  LKICNYIFTVIFCPLSQFSNLWAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIM
  RVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGR
  HATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLM
  KHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASGF
  SLEHPTMVPHPEEVPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILS
  VHSQPADTSCILQLPKDVHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSRED
  LLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGIQSKVSKHIRLPAPCPGLEPSWAKDPPETRSSLELDTEL
  SWISGDLLPSSQEEPLSPRDLKKCYSVETQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGG
  QPLGGPGSRPKKKLSPPSISIDPPESQGSRPPCSPGVCLRRRAPASDSKDPSVSSPLDSTAASPSPKKDT
  LSLSGLSSDPTDMDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031601
• ORF Size: 6765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031601.4, NP_113789.3
• RefSeq Size: 7657 bp
• RefSeq ORF: 6768 bp
• Locus ID: 29717
• Cytogenetics: 10q26
• MW: 250.3 kDa
• Gene Summary: low voltage-activated calcium channel alpha subunit; involved in calcium ion transport in nerve, muscle and edocrine cells [RGD, Feb 2006]