Cacna1g (NM_031601) Rat Untagged Clone

CAT#: RN211239

Cacna1g (untagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211239 representing NM_031601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGTAGCTTCACGCAGCTCAACG
  ACCTGTCCGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCGTACCCGGCGCTAGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGTATGCTGGTCATTCTTCTCA
  ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAGCGCTGCCGGATCCT
  GCAGGCCTTCGATGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTGGCCTTGGGC
  ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATTGTCATTGCAGGGA
  TGCTGGAGTATTCGCTGGACCTGCAGAACGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGACACCTTGCCTATG
  CTGGGCAACGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTGGGCGTCCAGCTGTGGG
  CAGGACTGCTTCGCAACCGATGCTTCCTCCCCGAGAACTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCC
  TTATTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAATGGCATGAGA
  TCCTGCAGGAGTGTGCCCACACTGCGTGGGGAAGGCGGTGGTGGCCCACCCTGCAGTCTGGACTATGAGA
  CCTATAACAGTTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTATACCAACTGCTCTGCGGGCGA
  GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACACTGGAGGGCTGGGTCGACATCATGTACTTCGTAATGGACGCTCACTCCTTCTACAACTTCATCT
  ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA
  GTTCTCCGAGACCAAACAGCGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT
  AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTACTCAAGTACCTGGTGTACATCC
  TCCGAAAAGCAGCCCGAAGGCTGGCCCAGGTCTCTAGGGCTATAGGCGTGCGGGCTGGGCTGCTCAGCAG
  CCCAGTGGCCCGTAGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCACTCGCTCACACCGTCGTCTGTCT
  GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG
  TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCTCGCCGGCTCATGCTACCACCACC
  CTCTACACCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC
  TGCCACTTGGAGCCAGTCCGTTGCCAGGCACCCCCTCCCAGATGCCCATCGGAGGCATCTGGTAGGACTG
  TGGGTAGTGGGAAGGTGTACCCCACTGTGCATACCAGCCCTCCACCAGAGATACTGAAGGATAAAGCACT
  AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC
  TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT
  GCTCCAAGGCAGACAGTGGAGCCTGCGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGAGA
  GCCAGAGTCCGCTGACCATGTCATGCCTGACTCAGACAGCGAGGCTGTGTATGAGTTCACACAGGACGCT
  CAGCACAGTGACCTCCGGGATCCCCACAGCCGGCGGCGACAGCGGAGCCTGGGCCCAGATGCAGAGCCTA
  GTTCTGTGCTGGCTTTCTGGAGGCTGATCTGTGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGG
  CCGGGGAATCATGATCGCCATCCTGGTCAATACACTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAG
  GAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGA
  AACTGCTTGTCTACGGTCCCTTTGGCTACATTAAGAATCCCTACAACATCTTTGATGGTGTCATTGTGGT
  CATCAGTGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATG
  CGGGTGCTGAAGCTGGTGCGCTTCCTGCCGGCCCTGCAGCGCCAGCTCGTGGTGCTCATGAAGACCATGG
  ACAACGTGGCCACCTTCTGCATGCTCCTCATGCTGTTCATCTTCATCTTCAGCATCCTGGGCATGCATCT
  CTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTCGACTCCCTG
  CTCTGGGCCATCGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTCTACAACGGCA
  TGGCCTCCACATCGTCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTATGTGCTCTT
  TAACCTGCTGGTGGCCATTCTTGTGGAAGGCTTCCAGGCAGAGGGAGATGCCACCAAGTCTGAGTCAGAG
  CCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGAAAGAAGCGCTTGGCCCTGGTGGCTTTGG
  GAGAACACGCGGAACTACGAAAGAGCCTTTTGCCACCCCTCATCATCCATACGGCTGCGACACCAATGTC
  ACTACCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGACGTACCAGTAGCAGT
  GGGTCCGCTGAGCCTGGAGCTGCCCACCATGAGATGAAATCTCCGCCAAGTGCCCGCAGCTCCCCGCACA
  GTCCCTGGAGTGCGGCAAGCAGCTGGACCAGCAGGCGCTCCAGCAGGAACAGCCTGGGCCGGGCCCCCAG
  CCTAAAGCGGAGGAGCCCGAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGCCAGGAGAGTCAGGAT
  GAGGAGGAAAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGACCATCGCCACAGGGGTTCCTTGG
  AACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACTCTGCAGGTGCCGGGGCTGCACCGCACAGCCAG
  CGGCCGGAGCTCTGCCTCTGAGCACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACC
  CTGAGGACTGATGACCCCCAACTGGATGGGGATGATGACAATGATGAGGGAAATCTGAGCAAAGGGGAAC
  GCATACAAGCCTGGGTCAGATCCCGGCTTCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCGGCCTATAT
  CTTTCCTCCTCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTG
  GTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCG
  CTGAGCGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTTCTAGCTGAAATGACAGTGAA
  GGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAATGTGCTGGACGGC
  TTGCTGGTGCTCATCTCCGTCATCGACATCCTGGTCTCCATGGTCTCCGACAGCGGCACCAAGATCCTTG
  GCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTGCGTCCACTCAGGGTCATCAGCCGGGCCCAGGGACT
  GAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATTTGCTGTGCC
  TTCTTCATCATTTTTGGAATTCTCGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGG
  ACACCAGGAACATCACTAACAAATCCGACTGCGCTGAGGCCAGCTACCGATGGGTCCGGCACAAGTACAA
  CTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGTTGGGTTGACATC
  ATGTATGATGGGCTGGATGCTGTGGGTGTGGATCAGCAGCCCATCATGAACCACAACCCCTGGATGCTGC
  TATACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTTGTCCTGAACATGTTTGTGGGCGTGGTGGTGGA
  GAACTTCCATAAGTGCAGACAGCACCAGGAGGAGGAGGAGGCGAGGCGGCGTGAGGAGAAGCGACTACGG
  AGGCTGGAGAAAAAGAGAAGGAGTAAGGAGAAGCAGATGGCCGAAGCCCAGTGCAAGCCCTACTACTCTG
  ACTACTCGAGATTCCGGCTCCTTGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGG
  TGTCATCGGGCTGAACGTGGTCACTATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCT
  CTGAAGATCTGCAATTACATCTTTACCGTCATCTTTTGTCCTTTGAGTCAGTTTTCAAACTTGTGGGCCT
  TTGGCTTCCGCCGTTTCTTCCAGGACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCAT
  GGGCATCACACTGGAGGAGATTGAGGTCAATGCTTCGCTGCCCATCAACCCCACCATCATCCGTATCATG
  AGGGTGCTCCGCATTGCTCGAGTTCTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACA
  CGGTGATGCAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCTCTTCATGTTATTGTTTTTCATCTTTGC
  AGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGTCGG
  CATGCCACCTTTAGGAACTTTGGTATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGA
  ATGGTATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTACAACACTGTCATCTCCCC
  TATCTACTTTGTGTCCTTCGTGCTGACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCTGTGCTGATG
  AAGCACCTGGAAGAAAGCAACAAAGAGGCCAAGGAGGAGGCCGAGCTCGAGGCCGAGCTGGAGCTGGAGA
  TGAAGACGCTCAGCCCGCAGCCCCACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCCGGGGTGGAGGGTGT
  CAACAGTCCTGACAGCCCTAAGCCTGGGGCTCCACACACCACTGCCCACATTGGAGCAGCCTCGGGCTTC
  TCCCTTGAGCACCCCACGATGGTACCCCACCCCGAGGAGGTGCCAGTCCCCCTAGGACCAGACCTGCTGA
  CTGTGAGGAAGTCTGGTGTCAGCCGGACGCACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAG
  CACTGCTGAGAGATCCCTAGGACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCC
  GTTCACTCCCAACCAGCAGACACCAGCTGCATCCTACAGCTTCCCAAAGATGTGCACTATCTGCTCCAGC
  CTCATGGGGCCCCCACCTGGGGCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAG
  GCCTCTCAGGCGCCAGGCAGCAATAAGGACTGACTCCCTGGATGTGCAGGGCCTGGGTAGCCGGGAAGAC
  CTGTTGTCAGAGGTGAGTGGGCCCTCCTGCCCTCTGACCCGGTCCTCATCCTTCTGGGGCGGGTCGAGCA
  TCCAGGTGCAGCAGCGTTCCGGCATCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCC
  AGGCCTGGAACCCAGCTGGGCCAAGGACCCTCCAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTG
  AGCTGGATTTCAGGAGACCTCCTTCCCAGCAGCCAGGAAGAACCCCTGTCCCCACGGGACCTGAAGAAGT
  GCTACAGTGTAGAGACCCAGAGCTGCAGGCGCAGGCCTGGGTCCTGGCTAGATGAACAGCGGAGACACTC
  CATTGCTGTCAGCTGTCTGGACAGCGGCTCCCAACCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGC
  CAACCTCTTGGGGGTCCTGGGAGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCC
  CGGAGAGCCAGGGCTCTCGGCCCCCATGCAGTCCTGGTGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGA
  CTCTAAGGATCCCTCGGTCTCCAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGACACG
  CTGAGTCTCTCTGGTTTGTCTTCTGACCCAACAGACATGGACCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031601
• Insert Size: 6768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031601.4, NP_113789.3
• RefSeq Size: 7657 bp
• RefSeq ORF: 6768 bp
• Locus ID: 29717
• Cytogenetics: 10q26
• Gene Summary: low voltage-activated calcium channel alpha subunit; involved in calcium ion transport in nerve, muscle and edocrine cells [RGD, Feb 2006]
  Transcript Variant: This variant (2) lacks two in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.