Phlpp2 (NM_001109131) Rat Tagged ORF Clone

CAT#: RR211191

Phlpp2 (Myc-DDK-tagged ORF) - Rat PH domain and leucine rich repeat protein phosphatase-like (Phlppl), (10 ug)

Product Images

Circular map for RR211191

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phlpp2
• Synonyms: Phlppl; RGD1562857
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211191 representing NM_001109131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGGTGGAGCCCGTGCCCGCGGGCTCGCTGGAGCCCCCGGAGCCACCTGAAGCGGCGGCGCCTC
  GCCGGCCCGGAGGTATTCGGGTCCTAAAGAGGAATATGAAACACAATGGGAGCAGAAATTGTTTGAATAG
  AAGAAGTAGGTTTGGCTCTCGAGAAAGAGACTGGCTAAGAGAAGATGTGAAGAGAGGCTGTGTTTACCTT
  TATGGAGCGGACACGACCACTGCCACTACAACCACCACCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCT
  CCTCTGATTTACATCTTGTCCTTTGCACAGTGGAGACACCAGCGTCAGAAATATGTGCTGGAGAGGGAAG
  AGAAAGTCTCTATCTACAGCTTCATGGAGATCTGGTCAGGAGACTGGAGCCCACTGAACGGCCTCTCCAG
  ATTGTTTACGACTACTTATCCAGGCTGGGGTTTGAAGATCCCGTGCGCATACAGGAGGAGGCTACGAACC
  CTGATCTCAGCTGTATGATTCGATTTTATGGTGAAAAGCCATGCCAGATGGATCATCTGGACCGAATCCT
  GCTGTCTGGCATCTATAACGTCCGCAAAGGGAAGACCCAGCTGCACAAATGGGCTGAGCGCCTCGTTGTT
  CTCTGTGGCACCTGCCTTATTGTCTCCTCAGTGAAGGATTGCCAAACTGGAAAGATGCACATCTTGCCAC
  TGGTTGGGGGAAAGATAGAAGAAGTGAAGCGCCGGCAACACTCCCTTGCTTTCAGCTCAGCTGGAGCCCA
  AGCTCAGACCTATCATGTCAGCTTCGAGACTTTGGCTGAGTACCAGAGATGGCAGCGGCAGGCGTCTAAG
  GTGGTGTCCCAGAGGATCAGCACCGTGGACCTCTCGTGTTACAGCCTTGAGGAGGTTCCTGAGCATCTCT
  TCTACAGTCAAGACATCACCTACCTCAACCTGCGACACAACTTCATGCAGTTAGAAAGACCAGGGGGCCT
  TGACACACTCTACAAATTTTCTCAACTGAAGGGCCTGAACTTGTCCCATAATAAGCTTGGACTGTTTCCT
  GTGTTGCTGTGTGAGATTTCTACCCTGACTGAACTCAACCTTTCTTGTAATGGATTTCATGACTTGCCAA
  GTCAGATTGGTAATCTGTTAAATCTTCAGACCCTCTGCCTTGATGGCAATGTTCTCACAGCTCTACCAGA
  TGAATTGGGAAACCTGCAGCAGCTTACTTCTCTGGGAATTTCCTTCAACAATTTTAGTCAAATTCCTGAA
  GTTTTGGAGAAACTCACCATGTTAGATAAAGTGGTTATGGCAGGAAACCGTTTGGAAATCCTGAACCTAG
  GGGTGCTGACCAGGATGAACCATGTTAAGCATGTGGATCTAAGGATGAACCACTTGAAAACGGTGATTAT
  TGAAAATCTGGAGGGAAATAAACACATCACTCACATGGATTTGCGGGACAATCAGTTGACTGATTTGGAC
  CTTAGCTCCTTATGTAGCTTGGAGCAGCTGCATTGTGAGCGGAACCAGTTGAGAGAGCTGACACTCAGTG
  GCTTCTCCCTTCGAAACCTCTACGCCAATTGGAACAAGCTGACAGCAGTGAATGTCTATCCAGTACCAAG
  CCTGCTCACTTCTTTGGAACTCTCCCGAAACTTGCTAGAATGTATCCCTGACTGGGCCTGTGAAGCAAAA
  AAGCTAGAAATACTAGATATAAGCTACAATCTGCTTACAGAGGTTCCCATGAGAATTCTGAGTAGTCTGA
  GTCTTCGGAAACTGATGGTAGGACACAACCACATCCATGTCCTTCCGGCACTGGTGGAGCACATTCCCCT
  CGAGGTACTGGATGTCCAGCATAACACTCTGTCCAGGCTGCCGGACACACTGTTCTCCAAGGCCTTAAAT
  CTCAGGTACTTGAATGCATCCGCAAATAGTCTGGAGTCTTTGCCTTCTGCGTGTGCTGGAGAGGAGAGTC
  TGAGTGTGCTGCAGCTGCTGTACCTGACCAACAACCTTCTGACAGACCAGTGCATACCTGTTTTGGTCGG
  GCACCCACACCTGCGTGTTCTGCACCTTGCAAACAACCAGCTACAGACCTTTCCTGCAAGCAAACTAAAC
  AAATTGGAGCAACTGGAAGAACTGAATCTAAGTGGGAACAAGCTTAAAGCTATTCCCACAACCATAGCAA
  ACTGCAAAAGACTGCACACCCTTGTCGCACATGCCAACAACATCAGCATTTTCCCAGAGATCCTGCAGCT
  GCCTCAGATTCAGTTTGTTGACCTGAGTTGCAATGATTTGACAGAAATACTAATTCCAGAGACTCTGCCT
  GCCACTTTACAAGACCTTGATCTTACAGGAAATACAAATCTGGTTCTGGAACACAAGACTTTGGACATGT
  TTAGCCATATCACAACACTGAAAATTGATCAGAAACCTTTACCGACCACAGATTCTACAGTTACATCAAC
  CTTCTGGAGCCATGGACTGGCCGAGATGGCTGGGCAGAGGAATAAACTCTGTGTGTCTGCTCTGGCTATG
  GATAACTTTGCAGAGGGCGTGGGGGCTGTGTACGGCATGTTTGATGGGGACAGGAATGAGGAACTCCCCC
  GTCTGCTGCAGTGTACAATGGCAGACGTGCTTTTGGAAGAGGTACAGCACTCAACAAGTGACACCGTTTT
  CATGGCCAATACCTTCCTGGTGTCTCACAGGAAATTAGGAATGGCTGGCCAGAAGCTAGGTTCCTCTGCT
  CTCCTGTGCTATATTCGACCTGACACTGCCGACCCAACAAGTAGTTTTAGCTTGACTGTAGCCAATGTTG
  GCACGTGCCAAGCTGTCCTGTGCCGAGGTGGAAAGCCAGTACCTCTTTCTAAAGTCTTCAGCTTGGAGCA
  TGACCCAGAGGAGGCACAGAGGGTGAAGGACCAGAAAGCTATCATAACAGAGGACAACAAGGTGAATGGG
  GTTACCTGCTGCACCCGGATGCTGGGATGTACATACCTCTACCCCTGGATCCTCCCCAAGCCTCACATAT
  CCTCCACTCCGCTTACCATTCAAGATGAATTGCTGATTTTGGGAAACAAAGCACTGTGGGAACACTTGTC
  CTATCTAGAAGCTGTCAGTGCAGTCCGTCATGTGCAAGACCCATTAGCTGCTGCTAAGAAGCTGTGCACA
  TTAGCACAGAGCTATGGTTGTCAGGACAATGTGGGGGCAATGGTGGTTTATTTGAACATTGGTGAAGAAG
  GCTGTACCTGTGAAATGCATGGGCTTGCCCTTCCAGGCCCTGTGGGATTTGCTTCAACAGCAACCATCAA
  GGATGCCCCCAAGCCAACCACTCCCTCCTCTAGCAGTGGGATTGCCTCAGAGCTGAGCAGTGAGGTGTCG
  ACCTCAGAGGTGAGCAGTGAGGTGGGTTCCACAGCGTCTGACGAACACAACACTGTGGGCCTGGAGACGA
  GCTTGCTTCCCAGGCCTGAGAGGCGCTGTAGTCTGCACCCTGTGCCCTCTGCAGGCACTTTCCAGCGCCA
  GCCTTCTTGTGCTACCTTTTCCAGCAATCAGTCTGACAATGGACTGGACAGTGACGATGACCAGCCTGTT
  GAAGGCGTCATAACCAATGGCAGCAGGGTTGAGGTAGAGGTAGACATTCATTGCTGCCGAGGCAGAGAGT
  CTGAGAACTCCCCTACTCTCCCGAAGAATTCTTCCTACCCCTGTTCAGAGGAACATGCTAGGGGGTTGGG
  TTTTGGGATCCGAAGACAGAACAGTGTGAATAGTGGTATACTTCTGCCAGTGAACAAAGACAAGATGGAG
  TTACAGAAGTCTCCCTCCACTTCTTGTTTGTATGGAAAGAAACTCTCCAATGGCTCCATTGTGCCCCTAG
  AAGACAGCCTGAACCTCATTGAGGTGGCCACAGAAGCACCCAAGAGGAAAACTGGCTATTTTGCTGCCCC
  CACTCAGCTGGAACCAGAGGATCAATTTGTTGTCCCCCGTGACCTGGAAGACGAAGTGAAGGAGCAAATG
  AAGCAGCACCAGGAGAGCAGGCCTGAGCCTGAACCCAGGGAAGAGGACCGGACAGAGCCCCTGCAGGAGT
  TTGATACAGCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211191 representing NM_001109131
  Red=Cloning site Green=Tags(s)
  
  MGEVEPVPAGSLEPPEPPEAAAPRRPGGIRVLKRNMKHNGSRNCLNRRSRFGSRERDWLREDVKRGCVYL
  YGADTTTATTTTTSSSSSSSSSSSSDLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQ
  IVYDYLSRLGFEDPVRIQEEATNPDLSCMIRFYGEKPCQMDHLDRILLSGIYNVRKGKTQLHKWAERLVV
  LCGTCLIVSSVKDCQTGKMHILPLVGGKIEEVKRRQHSLAFSSAGAQAQTYHVSFETLAEYQRWQRQASK
  VVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLRHNFMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFP
  VLLCEISTLTELNLSCNGFHDLPSQIGNLLNLQTLCLDGNVLTALPDELGNLQQLTSLGISFNNFSQIPE
  VLEKLTMLDKVVMAGNRLEILNLGVLTRMNHVKHVDLRMNHLKTVIIENLEGNKHITHMDLRDNQLTDLD
  LSSLCSLEQLHCERNQLRELTLSGFSLRNLYANWNKLTAVNVYPVPSLLTSLELSRNLLECIPDWACEAK
  KLEILDISYNLLTEVPMRILSSLSLRKLMVGHNHIHVLPALVEHIPLEVLDVQHNTLSRLPDTLFSKALN
  LRYLNASANSLESLPSACAGEESLSVLQLLYLTNNLLTDQCIPVLVGHPHLRVLHLANNQLQTFPASKLN
  KLEQLEELNLSGNKLKAIPTTIANCKRLHTLVAHANNISIFPEILQLPQIQFVDLSCNDLTEILIPETLP
  ATLQDLDLTGNTNLVLEHKTLDMFSHITTLKIDQKPLPTTDSTVTSTFWSHGLAEMAGQRNKLCVSALAM
  DNFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLLEEVQHSTSDTVFMANTFLVSHRKLGMAGQKLGSSA
  LLCYIRPDTADPTSSFSLTVANVGTCQAVLCRGGKPVPLSKVFSLEHDPEEAQRVKDQKAIITEDNKVNG
  VTCCTRMLGCTYLYPWILPKPHISSTPLTIQDELLILGNKALWEHLSYLEAVSAVRHVQDPLAAAKKLCT
  LAQSYGCQDNVGAMVVYLNIGEEGCTCEMHGLALPGPVGFASTATIKDAPKPTTPSSSSGIASELSSEVS
  TSEVSSEVGSTASDEHNTVGLETSLLPRPERRCSLHPVPSAGTFQRQPSCATFSSNQSDNGLDSDDDQPV
  EGVITNGSRVEVEVDIHCCRGRESENSPTLPKNSSYPCSEEHARGLGFGIRRQNSVNSGILLPVNKDKME
  LQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATEAPKRKTGYFAAPTQLEPEDQFVVPRDLEDEVKEQM
  KQHQESRPEPEPREEDRTEPLQEFDTAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109131
• ORF Size: 4074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109131.1, NP_001102601.1
• RefSeq Size: 4148 bp
• RefSeq ORF: 4077 bp
• Locus ID: 498949
• Cytogenetics: 19q12
• MW: 150.3 kDa