Nop14 (NM_001106013) Rat Tagged ORF Clone

CAT#: RR211187

Nop14 (Myc-DDK-tagged ORF) - Rat nucleolar protein 14 (Nol14), (10 ug)

Product Images

Circular map for RR211187

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nop14
• Synonyms: Nol14; RGD1305605
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211187 representing NM_001106013
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAAGCGAAGCGGACTGGGGCGAGGAGGCAGGTCCACAAAGCGCCGGCAGGGGCGTTCGGAGGCC
  CGGGGAAGACTAACCCAAATCCATTTGAAGTGAAAGTCAACCGGCAGAAGTTCCAGATCCTTGGCAGGAA
  GACACGCCACGATGTGGGCCTGCCCGGGGTGTCCCGCGCTCGGGCCATCAAGAAGAGAACCCAGACTTTA
  CTTAAGGAGTACAAAGAAAGGAACAAGTCCAATGTCTTCACAGATAAACGCTTTGGGGAGTATAATAGTA
  ACATAAGTCCGGAGGAAAAAATGATGAAGAGATTTGCTCTGGAGCAACAGCGATATCATGAGAAAAAGAA
  CATCTACAACCTAAATGAGGATGAAGAATTGACTCATTACGGCCAGTCCTTGGCTGACATTGAAAAGCAC
  AATGACATCGTGGATAGTGACAGTGACACCGAAGACCGGGGGGCACTGTCTGCTGAACTGACTGCTTCCC
  ACTTTGGTGGTGGGGTCCACAAGAATTCCTCTCAGAAGGAAGGAGAGGATGGAGACAAGCCAAAGACACG
  GAAAGAGCTGATTGAAGAGCTCATTGCAAAGTCAAAGCAAGAGAAGAGGGAAAGACAGGCTCAACGAGAA
  GATGCCCTTGAGCTCACAGAGAAGCTAGACCAAGACTGGAAAGAAATCCAGATTCTCCTGTCACGCAAAT
  CTAAGAAGTCAGAAAACAAAGAGAAAAAGGAAAAACCCCAGCCCGATGCTTATGACATGATGGTCCGTGA
  GCTTGGCTTTGAAATGAAGGCACAGCCCTCCAACAGGATGAAGACGGAAGAGGAACTGGCAAAGGAGGAG
  CAAGAGAAGCTCAGGAAATTGGAGGCCGAAAGACTCCGAAGAATGCTTGGAAAAGACGAACATGAAAATA
  AAAAGAAACCAAAACATACGTCGGCAGATGACTTAAATGATGGCTTTATACTAGATAAAGACGATAGGCG
  TTTGCTTTCTTACAAGGATGGTAAAATGAACATCGAGGATGCCCAGGAAGAGCAAAGCAAGGGAGCAGAT
  GGCCAAGAGAATGACCAGAAAGAGGAAGAAGATGAGAGTGAGGAAGAGGGTGAAGATGGTGAGAGCTGTG
  AGGACCCAGACGAGAGCGATGGCCCAGACAGCCACTCAGACCTAGAATCCAGTGCAGAGACCGAGGAGGA
  AGACGAGACACCGAAGAAAGAGCAGCGTCAGACTCCGGGGGGGAAGTCGCCGAAGGAGGCTCAGAAGGCT
  CAGAAGGCTGCTGCGGCCGAGCTGCCCTATGTCTTCGCAGCTCCTGAATCCTTTGAGGAGCTGAAGTTTT
  TATTATCAGGAAGATCAATGGAAGAACAGCTTTTGGTGGTGGAGAGAATTCAGAAGTGCAACCATCCGAG
  TCTTGCAGTAGGAAACAAAGCAAAACTGGAAAAATTGTTCGGCTTCCTTTTGCAATACATAGGCGATTTG
  GCCACAGACAGTACACCAGACCTCAAGACCATCGATAAGTTGGTTGTGCACTTATATAATCTGTGCCAGA
  TGTTTCCTGAATCTGCAAGTGATGCCATAAGATTTGTTCTCCGAGATGCCATGCACGAGATGGAAGAAAT
  GATTGAGACCAAAGGCCGGGCAGCATTTCCAGGGTTGGATGTGCTCATTTATTTGAAAATCACTGGGCTG
  TTATTTCCAACCTCCGATTTCTGGCATCCAGTTGTGACACCTGCCCTGATCTGTATGAGCCAGATGCTTA
  CCAAGTGTCCAGTCACGTCCCTGCGAGATGTGGTAAAGGGCCTGTTCGTGTGCTGCTTATTCCTGGAGTA
  TGTGTCTCTGTCTCGGAGGTTCGTCCCTGAGCTTCTAAATTTTCTTCTTGGGATTCTTTATATAGCAACT
  CCAAACAAGAAGAGCCAAGGCTGTCCTGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211187 representing NM_001106013
  Red=Cloning site Green=Tags(s)
  
  MGKAKRTGARRQVHKAPAGAFGGPGKTNPNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARAIKKRTQTL
  LKEYKERNKSNVFTDKRFGEYNSNISPEEKMMKRFALEQQRYHEKKNIYNLNEDEELTHYGQSLADIEKH
  NDIVDSDSDTEDRGALSAELTASHFGGGVHKNSSQKEGEDGDKPKTRKELIEELIAKSKQEKRERQAQRE
  DALELTEKLDQDWKEIQILLSRKSKKSENKEKKEKPQPDAYDMMVRELGFEMKAQPSNRMKTEEELAKEE
  QEKLRKLEAERLRRMLGKDEHENKKKPKHTSADDLNDGFILDKDDRRLLSYKDGKMNIEDAQEEQSKGAD
  GQENDQKEEEDESEEEGEDGESCEDPDESDGPDSHSDLESSAETEEEDETPKKEQRQTPGGKSPKEAQKA
  QKAAAAELPYVFAAPESFEELKFLLSGRSMEEQLLVVERIQKCNHPSLAVGNKAKLEKLFGFLLQYIGDL
  ATDSTPDLKTIDKLVVHLYNLCQMFPESASDAIRFVLRDAMHEMEEMIETKGRAAFPGLDVLIYLKITGL
  LFPTSDFWHPVVTPALICMSQMLTKCPVTSLRDVVKGLFVCCLFLEYVSLSRRFVPELLNFLLGILYIAT
  PNKKSQGCPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106013
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106013.1, NP_001099483.1
• RefSeq Size: 2537 bp
• RefSeq ORF: 1923 bp
• Locus ID: 289724
• Cytogenetics: 14q21
• MW: 72.9 kDa