Hspa8 (NM_024351) Rat Tagged ORF Clone

CAT#: RR211007

Hspa8 (Myc-DDK-tagged ORF) - Rat heat shock protein 8 (Hspa8), (10 ug)

Product Images

Circular map for RR211007

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa8
• Synonyms: Hsc70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211007 representing NM_024351
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGGGACCTGCAGTTGGCATTGATCTTGGCACCACCTACTCCTGTGTGGGTGTCTTCCAGCATG
  GAAAGGTGGAAATAATTGCCAATGACCAGGGTAACCGCACCACGCCGAGCTATGTTGCTTTCACCGACAC
  AGAACGATTAATTGGGGATGCGGCCAAGAATCAGGTTGCAATGAACCCCACCAACACAGTTTTTGATGCC
  AAACGTCTGATCGGACGTAGGTTCGATGATGCTGTTGTTCAGTCTGACATGAAGCACTGGCCCTTCATGG
  TGGTGAACGATGCAGGCAGGCCCAAGGTCCAAGTCGAATACAAAGGGGAGACAAAAAGTTTCTATCCTGA
  GGAAGTGTCTTCAATGGTTCTGACAAAAATGAAGGAAATTGCAGAAGCTTACCTTGGAAAGACTGTTACC
  AATGCCGTGGTCACCGTGCCAGCTTACTTCAATGACTCTCAGCGACAGGCAACAAAAGATGCTGGAACTA
  TTGCTGGCCTCAACGTACTTCGAATTATCAATGAGCCAACTGCTGCTGCTATTGCCTATGGCTTAGATAA
  GAAGGTCGGGGCTGAAAGGAATGTGCTCATTTTTGACTTGGGAGGTGGCACTTTTGATGTGTCAATCCTC
  ACTATCGAGGATGGAATTTTTGAAGTCAAATCAACAGCTGGAGACACCCACTTGGGCGGAGAAGACTTTG
  ACAACCGAATGGTCAACCATTTCATTGCTGAGTTTAAGCGAAAGCACAAGAAGGACATCAGTGAGAACAA
  GAGAGCTGTCAGGCGTCTCCGCACTGCCTGTGAGCGGGCCAAGCGCACCCTCTCCTCCAGCACCCAGGCC
  AGTATTGAGATTGATTCTCTCTATGAGGGAATTGACTTCTACACCTCCATTACCCGTGCTCGATTTGAGG
  AGTTGAATGCTGACCTGTTCCGTGGCACACTGGACCCTGTAGAGAAGGCCCTTCGAGATGCCAAACTAGA
  CAAGTCACAGATCCATGATATTGTCCTGGTGGGTGGTTCTACCAGAATCCCCAAGATCCAGAAACTTCTG
  CAAGACTTCTTCAATGGAAAAGAGCTGAATAAGAGCATTAACCCCGATGAAGCTGTTGCCTATGGTGCAG
  CTGTCCAGGCAGCCATTCTATCTGGAGACAAGTCTGAGAATGTTCAGGATTTGCTGCTCTTGGATGTCAC
  TCCTCTTTCCCTTGGGATTGAAACTGCTGGTGGAGTCATGACTGTCCTCATCAAGCGCAATACCACCATT
  CCCACCAAGCAGACCCAGACTTTCACCACCTACTCTGACAACCAGCCAGGTGTACTCATCCAGGTGTATG
  AAGGTGAAAGGGCCATGACCAAGGACAACAACCTGCTTGGGAAGTTTGAGCTCACAGGCATACCTCCAGC
  ACCCCGTGGGGTTCCTCAGATTGAGGTTACTTTTGACATTGATGCCAATGGCATCCTCAATGTTTCTGCT
  GTAGATAAGAGCACAGGAAAGGAGAACAAGATCACCATCACCAATGACAAGGGCCGCTTGAGTAAGGAGG
  ATATTGAGCGCATGGTCCAAGAAGCTGAGAAGTACAAAGCTGAGGATGAGAAGCAGAGAGATAAGGTTTC
  CTCTAAGAACTCGCTGGAGTCTTATGCTTTCAACATGAAAGCAACTGTTGAGGATGAGAAACTTCAAGGC
  AAGATCAATGATGAAGACAAACAGAAGATTCTTGACAAGTGCAACGAAATCATCAGCTGGCTGGATAAGA
  ACCAGACTGCGGAGAAGGAAGAATTTGAGCATCAGCAGAAAGAACTGGAGAAGGTCTGCAACCCTATCAT
  CACCAAGCTGTACCAGAGTGCTGGTGGCATGCCTGGAGGAATGCCTGGTGGCTTCCCTGGTGGAGGAGCT
  CCTCCATCTGGTGGTGCTTCTTCAGGCCCCACCATTGAAGAGGTCGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211007 representing NM_024351
  Red=Cloning site Green=Tags(s)
  
  MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
  KRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVT
  NAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSIL
  TIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQA
  SIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLL
  QDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTI
  PTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSA
  VDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQG
  KINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGA
  PPSGGASSGPTIEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024351
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024351.2, NP_077327.1
• RefSeq Size: 2130 bp
• RefSeq ORF: 1941 bp
• Locus ID: 24468
• UniProt ID: P63018
• Cytogenetics: 8q22
• MW: 70.9 kDa
• Gene Summary: member of the hsp70 protein; expressed in unstressed cells [RGD, Feb 2006]