Cdk4 (NM_053593) Rat Tagged ORF Clone

CAT#: RR210857

Cdk4 (Myc-DDK-tagged ORF) - Rat cyclin-dependent kinase 4 (Cdk4), (10 ug)

Product Images

Circular map for RR210857

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210857 representing NM_053593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCACTCGATATGAACCCGTGGCTGAAATTGGTGTCGGTGCCTATGGGACGGTGTACAAAGCCC
  GAGATCCCCACAGTGGCCACTTTGTGGCTCTCAAGAGTGTGAGAGTTCCTAATGGAGGAGCAGCTGGAGG
  GGGCCTTCCCGTCAGCACAGTTCGTGAGGTGGCCTTGTTAAGAAGGCTGGAGGCCTTTGAACATCCCAAT
  GTTGTACGGCTGATGGATGTCTGTGCTACTTCCCGAACTGATCGGGACATCAAGGTCACCTTAGTGTTTG
  AGCATATAGACCAGGACCTACGGACATACCTGGACAAAGCACCTCCGCCGGGCTTGCCTGTTGAGACCAT
  TAAGGATCTGATGCGCCAGTTTCTAAGCGGCCTAGATTTCCTTCATGCAAACTGCATTGTTCACCGGGAC
  CTGAAGCCAGAGAACATTCTAGTGACAAGTAATGGGACAGTTAAGCTGGCCGACTTTGGCCTAGCCAGAA
  TCTACAGCTACCAGATGGCCCTCACGCCTGTGGTTGTTACGCTCTGGTACCGGGCTCCTGAAGTTCTTCT
  GCAGTCTACATATGCAACGCCTGTGGATATGTGGAGTGTTGGCTGTATCTTCGCAGAGATGTTTCGCCGG
  AAGCCTCTCTTCTGTGGGAACTCTGAGGCTGACCAGCTGGGCAAAATCTTTGATCTCATTGGATTGCCTC
  CAGAAGACGACTGGCCTCGAGAGGTCTCTCTTCCTCGAGGAGCCTTTTCCCCCAGAGGACCTCGGCCAGT
  GCAGTCAGTGGTGCCGGAGATGGAGGAATCTGGAGCGCAGTTGCTGCTGGAAATGCTGACCTTTAATCCA
  CTTAAGCGAATCTCTGCCTTCCGAGCCCTGCAGCACTCTTACCTGCACAAGGAGGAAAGTGACCCGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210857 representing NM_053593
  Red=Cloning site Green=Tags(s)
  
  MATTRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGAAGGGLPVSTVREVALLRRLEAFEHPN
  VVRLMDVCATSRTDRDIKVTLVFEHIDQDLRTYLDKAPPPGLPVETIKDLMRQFLSGLDFLHANCIVHRD
  LKPENILVTSNGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRR
  KPLFCGNSEADQLGKIFDLIGLPPEDDWPREVSLPRGAFSPRGPRPVQSVVPEMEESGAQLLLEMLTFNP
  LKRISAFRALQHSYLHKEESDPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053593
• ORF Size: 909 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053593.2, NP_446045.1
• RefSeq Size: 1395 bp
• RefSeq ORF: 912 bp
• Locus ID: 94201
• UniProt ID: P35426
• Cytogenetics: 7q22
• MW: 33.8 kDa
• Gene Summary: positive regulator of the cell cycle; plays a role in cell cycle progression [RGD, Feb 2006]