Asic5 (NM_022227) Rat Tagged ORF Clone

CAT#: RR210785

Asic5 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 5, intestinal (Accn5), (10 ug)

Product Images

Circular map for RR210785

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asic5
• Synonyms: Accn5; Blinac; Inac
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210785 representing NM_022227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACATACTGAGAAATCAAAAGGGCCCGCCGAGAAAGGACTCTTAGGAAAGATAAGACGTTACCTCT
  CAAAGAGACCACTGCCATCTCCAACTGACCGGAAGAAGTTTGATCATGACTTTGCCATCTCCACTTCCTT
  TCATGGGATACATAATATTGCCCAGAACCAGAACAAGGTTCGAAAGGTGATCTGGCTGTCTGTGGTGCTG
  GGCTCTGTCTCGCTCTTGGTGTGGCAAATCTACAGTCGCTTGGTCAATTATTTCATGTGGCCAACGACAA
  CATCTATAGAAGTTCAGTATGTGGAGAAAATTGAGTTCCCGGCTGTGACATTCTGTAACTTGAACAGATT
  TCAAACAGAAGCAGTATCCAGATTTGGCATCATTTTCTTCTTATGGGACATAGTCTCCAAAGTCCTCCGC
  CTTCAGGAAATCAGTGGCAACAACACTGGCTCTCCAGAGGCCTTAGATTTTGTTGCAAGTCACCGAAACT
  TCAGCATTACAGAGTTTGTTAAGAACAATGGTTTTTATCTCAATCATGACACTTTGGTGCACTGTGAGTT
  CTTTGGAAAAACATGTGATCCAAAGGATTTCAAACACGTCTTTACTGAATATGGGAATTGCTTCACTTTC
  AACTATGGCGAGAATGTCCAAAGCAAGAACAAAGTGAGTGTGTCTGGAAGAGGCTTGAAGCTGCTGCTTG
  ATGTCCATCAGGAGGAATTCACGGACAACCCAGTCCCTGGTTTTGCTGATGCCGGGGTCATCTTTGTCAT
  TCACTCACCGAAGAAGGAGCCCCAGTTCGATGGCTTGGGTTTGTCTTCTCCTGTGGGGATGCATGCACGG
  GTGACCATCCGCCAACTGAAGACCATCCACCAGGAGTACCCATGGGGAGAATGCAATCCTGACATCAAGT
  TGAGGAACTTTACCACCTACAGCACGTATGGTTGTCTGAAGGAGTGCAAAGCCAAGCACATCCAAAGGCT
  GTGTGGATGTTTGCCCTTTCTGCTTCCAGGAAATGGTGTAGAGTGTGACCTACTAAAATACTACAACTGT
  GTGTCTCCCATCCTTGACCACATCGAGCGTAAAGGGCTGTGCACAATGGGAACACACAACTCCAGCTGCC
  CCGTGCCATGCGAAGAAACAGAATACCCTGCTACAATTGCTTATTCGACTTTCCCAAGTCAAAGAGCAAC
  AAAATTTCTTGCCAAGAAATTGAATCAAAGCCAAGAATATATCCGGGAGAATCTTGTGAACATTGAAATA
  AACTATAGCGACTTAAACTATAAGATAACACAGCAACAAAAGGCAGTGAGTGTGCCCGAGTTACTTGCAG
  ATGTTGGAGGTCAGCTGGGACTATTTTGCGGAGCTAGTTTGATTACAATTATAGAGATTATTGAATATCT
  ATTCACCAGTTTCTACTGGGTATTCATTTTCTTTTTGCTGAAAATACTTGAAATGATCCAGCGGACTTCT
  CCACCTCAGACAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210785 representing NM_022227
  Red=Cloning site Green=Tags(s)
  
  MEHTEKSKGPAEKGLLGKIRRYLSKRPLPSPTDRKKFDHDFAISTSFHGIHNIAQNQNKVRKVIWLSVVL
  GSVSLLVWQIYSRLVNYFMWPTTTSIEVQYVEKIEFPAVTFCNLNRFQTEAVSRFGIIFFLWDIVSKVLR
  LQEISGNNTGSPEALDFVASHRNFSITEFVKNNGFYLNHDTLVHCEFFGKTCDPKDFKHVFTEYGNCFTF
  NYGENVQSKNKVSVSGRGLKLLLDVHQEEFTDNPVPGFADAGVIFVIHSPKKEPQFDGLGLSSPVGMHAR
  VTIRQLKTIHQEYPWGECNPDIKLRNFTTYSTYGCLKECKAKHIQRLCGCLPFLLPGNGVECDLLKYYNC
  VSPILDHIERKGLCTMGTHNSSCPVPCEETEYPATIAYSTFPSQRATKFLAKKLNQSQEYIRENLVNIEI
  NYSDLNYKITQQQKAVSVPELLADVGGQLGLFCGASLITIIEIIEYLFTSFYWVFIFFLLKILEMIQRTS
  PPQTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022227
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022227.1, NP_071563.1
• RefSeq Size: 1887 bp
• RefSeq ORF: 1488 bp
• Locus ID: 63866
• UniProt ID: Q9R0W5
• Cytogenetics: 2q34
• MW: 56.5 kDa
• Gene Summary: may play a critical role in fluid and electrolyte homeostasis [RGD, Feb 2006]