Pdcd2l (NM_001109544) Rat Tagged ORF Clone

CAT#: RR210523

Pdcd2l (Myc-DDK-tagged ORF) - Rat programmed cell death 2-like (Pdcd2l), (10 ug)

Product Images

Circular map for RR210523

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdcd2l
• Synonyms: RGD1308249
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210523 representing NM_001109544
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTCAGGAAGCCGGTGCTGCTGGGATTTCGAGACTCGGCGGTGAAGGGCTGCCCCAAGGGAC
  CCGCCGCCTGGACGGCTAGCAAGATAGGCGGCGTTCCGGACGCCCTGCCGGCCGTTACTGCCCCGGGCCC
  GCGGTGTGACCGCTGCGCACAGCCGCTCGCGCTCGTCGTGCAGGTGTACTGCCCGCTGGAAGGCTCCCCG
  TTCCATCGGCTTCTGTACGTTTTCGCCTGTGCCCGTCGGGACTGCAGCGACAACCAGACGCGCAGCTGGA
  AGGTGTTCCGCTCCCAGTGCCTGCAAGTGCCAGAGAAAGAGACTCGGAACGGACAGAATCAGAGTGAAGG
  CCTTGCCGCTGAGAACTGGTGTGAGGGCTCACATGACTGGGGAAGTGATACCGAGGAGACGCCTCCGCCA
  CCACCCCCAGCAGATTTGGGAAGGGATTCTAATGATGCCAGAGCGCTAGACTGGACTGACAAGCTCCAAG
  CTCTTCACCTGCAGGATAGTGCCCTGGCTGTTGCCTGCCCTTCCCCTTCAGTCCCCACGGAAGTGCCACA
  GTTCCAGCCCTATTATATCAGTGTTGCCGAAGAGGATGAGTACGGCAACGTTGCGGATCTTGACCACGCC
  CACAGCCTCCTGCAGGAGTACCAGCAGAGAGAAGGTGTTGACATGCAGCAGCTGCTCTCCGTGGGTTGCA
  GTGATGGGGATGAAAAATACGAGAAGACCAAAGTTAGCAGTGGAGATCCAACGTTTTACAAGTTCATGAA
  GAGGATCTCTGTTTGCCAGGGGCAGATTCTGAGGTATTCATGGAGTGGAGAGCCTCTCTTTCTGTCCTGC
  CCCACATTTGAAGTCTCGGAGGTCCCAGTCTGCAGTGGCTGCGGAGGCCAGAGGGCGTTTGAGTTTCAGC
  TTATGCCAGCACTGGTCAGCATGCTCAACAGTACTAATCTAGGTCTCACTGTGGAATTTGGGACGATTCT
  GGTTTACACCTGTAAGCAGAGTTGCTGGCCTCCAAATCAGCAGATGCCCATGGAAGAATTCTGTGTTATA
  CAAGAAGACCCAGATGCATTTTTATTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210523 representing NM_001109544
  Red=Cloning site Green=Tags(s)
  
  MAAVRKPVLLGFRDSAVKGCPKGPAAWTASKIGGVPDALPAVTAPGPRCDRCAQPLALVVQVYCPLEGSP
  FHRLLYVFACARRDCSDNQTRSWKVFRSQCLQVPEKETRNGQNQSEGLAAENWCEGSHDWGSDTEETPPP
  PPPADLGRDSNDARALDWTDKLQALHLQDSALAVACPSPSVPTEVPQFQPYYISVAEEDEYGNVADLDHA
  HSLLQEYQQREGVDMQQLLSVGCSDGDEKYEKTKVSSGDPTFYKFMKRISVCQGQILRYSWSGEPLFLSC
  PTFEVSEVPVCSGCGGQRAFEFQLMPALVSMLNSTNLGLTVEFGTILVYTCKQSCWPPNQQMPMEEFCVI
  QEDPDAFLFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109544
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109544.1, NP_001103014.1
• RefSeq Size: 1253 bp
• RefSeq ORF: 1083 bp
• Locus ID: 689637
• Cytogenetics: 1q21
• MW: 39.7 kDa