Gnl3 (NM_175580) Rat Tagged ORF Clone

CAT#: RR210460

Gnl3 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein-like 3 (nucleolar) (Gnl3), (10 ug)

Product Images

Circular map for RR210460

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gnl3
• Synonyms: Ns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210460 representing NM_175580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGCCGAAGTTAAAGAAAGCAAGTAAACGTATGACCTGTCATAAGCGGTATAAAATCCAGAAAA
  AGGTTCGAGAACATCATCGAAAATTAAGGAAGGAAGCTAAAAAGCGGGGTCACAAGAAGCCTAAGAAGGA
  CCCAGGAGTTCCAAATAGTGCTCCCTTTAAAGAGGCTCTTCTTCGTGAAGCTGAGCTAAGGAAACAGCAG
  CTTGAAGAACTAAAACAGCAGCAGAAACTTGACAGGCAAAAAGAACAAGAACGAAAAAGAAAACTTGAAA
  TTAGCCCTGATGATGAGCAATCTAATGTGGAAACTCAGGAGGAATCTGATGAGCCCAAAATAAAGAAAGC
  TAAATCAGGCAAACAGAATCCAAAGAAGTTACATTGTCAGGAACTTAAAAAGGTGATTGAAGCCTCAGAC
  ATTGTGTTAGAAGTTTTGGATGCCAGAGATCCTCTTGGTTGCAGGTGTCCTCAAGTAGAAGAAGCTGTTA
  TCCAAAGTGGATGTAAAAAACTAGTACTTGTATTAAATAAGTCAGATCTAGTACCAAAAGAGAATCTAGA
  GAACTGGCTAACTTACTTGAATAAGGAATTGCCAACAGTGGTGTTCAAAGCCTCAACAAACTTAAAGAAC
  AGAAAGAAGACATTCAAGATAAAGAAGAAAGTTGTTCCATTCCAAAGTAAACTCTGCTGTGGCAAGGAAG
  CACTGTGGAAGCTCCTTGGAGGTTTTCAGCAGTCCTGTGGAAAAGGAGTTCAGGTTGGAGTGGTTGGTTT
  CCCAAATGTGGGAAAAAGCAGCATCATTAATAGTTTAAAACAAGAAAGGATTTGCAGTGTTGGAGTTTCC
  ATGGGACTTACAAGGAGTATGCAGATTGTCCCTTTAGACAAACAGATCACAATCATAGATAGTCCGTGCT
  TCATTATCTCACCTTGTAACTCCCCTGCTGCACTTGCCCTCCGAAGTCCAGCAAGTATTGAAGTTCTAAG
  ACCATTGGAGGCTGCCAGTGCCATCCTGTCTCAGGCTGATAGTCAACAGGTGGTGTTAAAATATACTGTC
  CCGGGGTATAAGGATTCTCTGGATTTTTTTACTAAACTTGCTCAGAGAAGAGGTCTGCACCAAAAAGGTG
  GAAGCCCAAATGTCGAAAGTGCTGCTAAGCTGCTATGGTCTGAGTGGACAGGTGCCTCATTAGGTTACTA
  CTGCCATCCCCCTGCATCCTGGAATCATTCTCCTCATTTTAATGAGAATATTACAGCAATCATGAAGAGG
  GGCTTTAATCTAGAAGAACTAGAAAAGAATAATGCACACAGCATACAAGTCCTCAAGGGCCCTCATTTAA
  CTAATAAAATCCTTTTCCGGTCTTCGGGCCTGACAAATGGAATACTAGAAGAAAAGGACATCCCCGAAGA
  GTCACCAAAACAGACAGAAGACCAACAGGATGGTGATGATCAAGAACATGTTACTGGTGAAAAAAATGCA
  GAGATCTCAGATGTGACTCCTGTAGAAGAGACCAGGGAGATGTCACCTGGGCAATCAACAGCAAGTAAAC
  CATCTGACAGATCCTTTATCTTGGATAAAATGAGTGAAGAAGACGATGCCTATGACTTTACCACAGATTA
  TATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210460 representing NM_175580
  Red=Cloning site Green=Tags(s)
  
  MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPKKDPGVPNSAPFKEALLREAELRKQQ
  LEELKQQQKLDRQKEQERKRKLEISPDDEQSNVETQEESDEPKIKKAKSGKQNPKKLHCQELKKVIEASD
  IVLEVLDARDPLGCRCPQVEEAVIQSGCKKLVLVLNKSDLVPKENLENWLTYLNKELPTVVFKASTNLKN
  RKKTFKIKKKVVPFQSKLCCGKEALWKLLGGFQQSCGKGVQVGVVGFPNVGKSSIINSLKQERICSVGVS
  MGLTRSMQIVPLDKQITIIDSPCFIISPCNSPAALALRSPASIEVLRPLEAASAILSQADSQQVVLKYTV
  PGYKDSLDFFTKLAQRRGLHQKGGSPNVESAAKLLWSEWTGASLGYYCHPPASWNHSPHFNENITAIMKR
  GFNLEELEKNNAHSIQVLKGPHLTNKILFRSSGLTNGILEEKDIPEESPKQTEDQQDGDDQEHVTGEKNA
  EISDVTPVEETREMSPGQSTASKPSDRSFILDKMSEEDDAYDFTTDYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175580
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175580.2, NP_783170.1
• RefSeq Size: 1810 bp
• RefSeq ORF: 1617 bp
• Locus ID: 290556
• UniProt ID: Q811S9
• Cytogenetics: 16p16
• MW: 60.7 kDa
• Gene Summary: involved in controlling cell proliferation in stem cells and cancer cells [RGD, Feb 2006]