Itgb6 (NM_001004263) Rat Tagged ORF Clone

CAT#: RR210305

Itgb6 (Myc-DDK-tagged ORF) - Rat integrin, beta 6 (Itgb6), (10 ug)

Product Images

Circular map for RR210305

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgb6
• Synonyms: MGC94023
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210305 representing NM_001004263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGATTGAGCTGGTCTGCCTGTTCCTGCTACTTCTAGGAAGGAATGATCACGTCCAAGGTGGCTGTG
  CCTGGAGTGGTGCAGAAACCTGCTCAGACTGCCTGCTCACAGGACCTCACTGCGCCTGGTGCTCCCAGGA
  GAATTTCACCCACCTGTCCGGAGCTGGCGAAAGGTGCGACACCCCCGAGAATCTTTTAGCCAAAGGATGT
  CAGTTACCCTTCATCGAAAACCCTGTCTCCCAAGTAGAAATACTTCAAAATAAGCCTCTCAGCGTAGGTC
  GACAGAAAAACAGTTCTGACATTGTTCAGATTGCTCCTCAAAGCTTGGTTCTTAAGTTGAGACCAGGGGG
  TGAGCAGACTCTGCAAGTGCAGGTCCGCCAGACGGAAGATTACCCAGTAGATCTGTATTACCTCATGGAC
  CTCTCGGCCTCCATGGATGATGACCTCAACACAATCAAAGAGCTTGGCTCCCGGCTGGCCAAAGAGATGT
  CTAAATTAACCAGCAACTTTAGACTGGGCTTTGGCTCTTTTGTGGAAAAGCCCGTTTCTCCTTTTATGAA
  AACAACACCAGAGGAAATCACCAACCCTTGCAGTAGTATCCCCTATTTCTGCTTACCTACATTTGGATTC
  AAGCACATTTTGCCATTGACTGATGATGCTGAGAGATTCAATGAAATTGTGAGGAAACAGAAGATTTCCG
  CTAATATTGACACGCCTGAAGGCGGATTCGATGCCATCATGCAAGCTGCTGTGTGTAAGGAAAAGATTGG
  CTGGCGCAACGACTCGCTCCACCTCCTGGTTTTTGTGAGTGATGCTGATTCTCATTTTGGAATGGACAGC
  AAGCTGGCAGGCATTGTGATTCCCAACGATGGGCTCTGTCACTTGGACAACAGGAATGAATACTCCATGT
  CAACTGTCTTGGAATATCCAACGATCGGCCAACTCATTGATAAACTGGTACAAAACAACGTGCTACTGAT
  CTTTGCGGTCACCCAAGAACAAGTCCATCTCTATGAGAATTATGCGAAACTCATCCCTGGAGCGACTGTG
  GGACTGCTTCAGAAGGATTCTGGGAACATTCTTCAGCTGATCATCTCCGCTTACGAAGAACTGCGGTCTG
  AGGTGGAACTAGAAGTATTAGGAGACACAGAAGGACTCAACCTGTCTTTCACAGCTCTCTGTAGCAATGG
  CATCCTCTTCCCACACCAAAAGAAGTGTTCCCACATGAAAGTCGGAGATACAGCATCCTTCAACGTGTCT
  GTGAGCATAACAAACTGTGAGAAAAGAAGCAGGAAACTTATCATCAAGCCTGTGGGACTTGGGGACACCC
  TGGAAATACTCGTCAGCGCAGAATGTGACTGCGACTGCCAGAGAGAAGTAGAAGCCAACAGTTCTAAGTG
  CCACCACGGGAATGGCTCCTTCCAGTGTGGGGTGTGTGCCTGCAACCCTGGCCACATGGGTCCTCGCTGC
  GAGTGCGGCGAGGACATGGTGAGCACGGATTCCTGCAAGGAGTCCCCAGGTCACCCCTCCTGCAGCGGAA
  GGGGTGACTGCTATTGTGGGCAGTGTGTCTGCCACTTGTCTCCCTATGGAAGCATCTATGGACCTTACTG
  CCAGTGTGACAACTTCTCCTGTCTGAGACACAAAGGCCTGCTCTGTGGAGATAATGGTGACTGTGACTGT
  GGTGAATGCGTGTGCCGGGATGGCTGGACGGGCGAGTACTGCAACTGTACAACCAGCAGGGATGCGTGTG
  CATCGGAAGATGGCGTGCTGTGCAGCGGGCGTGGGGACTGTGTCTGCGGCAAGTGTGTCTGCAGAAACCC
  TGGAGCCTCGGGACCCACCTGTGAACGCTGCCCTACCTGTGGAGACCCCTGTAACTCGAGACGGAGCTGC
  ATCGAGTGCTACCTGTCTGCAGATGGCCAGGCCCAAGAAGAGTGCGAGGACAAGTGCAAAGCCACCGGTG
  CCACCATCAGTGAGGAAGAGTTTTCAAAGGATACTTCTGTCCCCTGCTCGCTACAAGGAGAAAATGAATG
  TCTTATTACATTCCTAATAACTGCGGATAATGAAGGGAAAACCATCATTCACAACATCAGTGAAAAAGAC
  TGCCCCAAACCTCCAAACATTCCCATGATCATGTTGGGGGTGTCACTGGCTATCCTGCTCATCGGTGTCG
  TGCTACTGTGCATTTGGAAGCTGCTGGTATCCTTTCACGACCGGAAAGAAGTTGCTAAATTTGAAGCAGA
  ACGATCAAAGGCCAAGTGGCAAACGGGAACCAATCCGCTGTACCGAGGCTCCACCAGCACTTTTAAGAAC
  GTGACCTACAAACACAGGGAGAAGCACAAAGTGGGCCTTTCTTCAGATGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210305 representing NM_001004263
  Red=Cloning site Green=Tags(s)
  
  MGIELVCLFLLLLGRNDHVQGGCAWSGAETCSDCLLTGPHCAWCSQENFTHLSGAGERCDTPENLLAKGC
  QLPFIENPVSQVEILQNKPLSVGRQKNSSDIVQIAPQSLVLKLRPGGEQTLQVQVRQTEDYPVDLYYLMD
  LSASMDDDLNTIKELGSRLAKEMSKLTSNFRLGFGSFVEKPVSPFMKTTPEEITNPCSSIPYFCLPTFGF
  KHILPLTDDAERFNEIVRKQKISANIDTPEGGFDAIMQAAVCKEKIGWRNDSLHLLVFVSDADSHFGMDS
  KLAGIVIPNDGLCHLDNRNEYSMSTVLEYPTIGQLIDKLVQNNVLLIFAVTQEQVHLYENYAKLIPGATV
  GLLQKDSGNILQLIISAYEELRSEVELEVLGDTEGLNLSFTALCSNGILFPHQKKCSHMKVGDTASFNVS
  VSITNCEKRSRKLIIKPVGLGDTLEILVSAECDCDCQREVEANSSKCHHGNGSFQCGVCACNPGHMGPRC
  ECGEDMVSTDSCKESPGHPSCSGRGDCYCGQCVCHLSPYGSIYGPYCQCDNFSCLRHKGLLCGDNGDCDC
  GECVCRDGWTGEYCNCTTSRDACASEDGVLCSGRGDCVCGKCVCRNPGASGPTCERCPTCGDPCNSRRSC
  IECYLSADGQAQEECEDKCKATGATISEEEFSKDTSVPCSLQGENECLITFLITADNEGKTIIHNISEKD
  CPKPPNIPMIMLGVSLAILLIGVVLLCIWKLLVSFHDRKEVAKFEAERSKAKWQTGTNPLYRGSTSTFKN
  VTYKHREKHKVGLSSDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004263
• ORF Size: 2361 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004263.1, NP_001004263.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 2364 bp
• Locus ID: 311061
• UniProt ID: Q6AYF4
• Cytogenetics: 3q21
• MW: 86 kDa
• Gene Summary: mouse homolog is involved in TGF-beta activation and regulation of macrophage metalloproteinase Mmp12 expression [RGD, Feb 2006]