Ppfia1 (NM_001106320) Rat Tagged ORF Clone

CAT#: RR210272

Ppfia1 (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (Ppfia1), (10 ug)

Product Images

Circular map for RR210272

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppfia1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210272 representing NM_001106320
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGCGAGGTGATGCCGACCATTAGTGAAGCGGAAGGACCCCCAGGAGGAGGTGGGAGCCATGGGT
  CAGGCTCCCCTTCCCAGCCAGACGCTGATTCGCATTTTGAGCAGCTCATGGTTTCCATGCTGGAAGAGCG
  AGACCGCCTTCTGGACACACTGAGAGAGACACAAGAAACGCTGGCATTAACCCAGGGGAAGTTGCATGAA
  GTTGGTCATGAAAGAGATTCTTTGCAGAGGCAGCTCAACACTGCACTTCCACAGGAGTTTGCTGCACTCA
  CGAAGGAACTCAATGTGTGTCGGGAGCAGCTTCTGGAGAGAGAGGAAGAAATTGCTGAGCTGAAGGCAGA
  GAGGAACAACACTAGGCTGCTGTTGGAGCACCTAGAGTGCCTTGTCTCCAGGCACGAGCGCTCTCTACGT
  ATGACAGTGGTGAAGAGGCAGGCACAGTCACCTGCGGGCGTGTCCAGTGAGGTGGAGGTTCTGAAAGCCC
  TGAAGTCACTGTTTGAGCACCACAAAGCCTTGGATGAGAAGGTACGAGAGCGATTACGAGTAGCTCTGGA
  GAGATGCAGCTTGTTAGAAGAGGAGCTAGGTGCCACCCACAAAGAGCTAATGATTCTTAAAGAACAGAAC
  AGTCAGAAGAAAGCACTAACAGAGGGAATGCTTGATGGGAACCACGAACAGGAAAATGCACCGAGCACCA
  ACGGCAAGAGATCTTCCGATGGCTCCTTGAGCCATGAGGATCTTGCCAAAGTGCTGGAGCTGCAGGAAGT
  CATAGACAGGCAGGCTCGAGAGCAGAGCCAGATGAAGGAGCGCCTGGCCTCCCTGTCCAGTCATGCAGCA
  GAGCTGGAAGAGGATCTGGACACAGCTAGAAAAGACCTCATCAAGTCTGAAGAGATGAATACGAAACTGC
  AGCGAGAAGTCCGAGAAGCAATGGCCCAGAAGGAGGATATGGAGGAGAGAATCACCACCCTTGAGAAACG
  CTACCTCGCCGCACAGCGTGAAGCCACGTCTGTGCATGACCTCAATGACAAACTTGAAAATGAAATTGCA
  AATAAAGACTCCATGCACCGACAGACAGAGGACAAGAACCGCCAGTTGCAAGAACGTCTGGAATTGGCAG
  AGCAGAAGCTGCAGCAGACCCTGCGCAAAGCTGAGACGCTGCCAGAGGTGGAGGCTGAGCTGGCCCAGAG
  GGTGGCTGCACTCTCCAAGGCTGAGGAGAGACATGGCAACATTGAAGAGAGGCTGCGGCAGATGGAGGCA
  CAGCTGGAGGAGAAGAATCAGGAGCTGCAGCGGGCAAGACAGAGAGAAAAGATGAATGAGGAACATAATA
  AGCGCCTGTCTGACACTGTGGACAAGTTGCTCTCAGAATCCAATGAGAGGCTGCAGCTGCATCTGAAGGA
  GAGAATGGCGGCGCTGGAGGACAAGAATTCTCTGCTAAGGGAAGTTGAAAATGCAAAGAAGCAACTAGAA
  GAGACCCAGCATGATAAGGACCAACTTGTCTTGAATATTGAAGCCCTGAAGGCTGAGCTGGAGCAGATGA
  GACTGAGAGGTTCTTCACTTCATCATGGCCGACCCCATTTGGGCAGTGTCCCGGACTTCAGGTTCTCTGT
  GGCAGACGGGCATGTGGATGCTTACAGCACCAGTGCAGTGTTGCGGCGCCCCCAGAAGGGCCGGCTGGCA
  GCGCTGCGGGACGAGCCCTCCAAGGTGCAGACCCTCAATGAACAGGACTGGGAACGGGCCCAACAAGCCA
  GCGTCTTGGCAAATGTGGCACAGGCATTTGAGAGTGATGTGGATGTGTCTGATGGTGAAGATGACCGAGA
  CACTCTGCTCAGCTCAGTTGATCTGCTGTCACCCAGTGGGCAAGCTGATGCCCAGACCCTGGCCATGATG
  CTTCAGGAACAGCTGGATGCGATCAACAAAGAGATCAGACTGATCCAAGAAGAGAAGGAGAATACAGAGC
  AGCGGGCAGAGGAGATCGAGAGCAGAGTGGGCAGTGGAAGCTTGGACAATCTTGGTCGTTTTAGATCAAT
  GAGCTCCATACCCCCCTACCCTGCTTCCTCGCTTGCCGGCTCCTCTCCTCCAGGCAGTGGTCGGTCCACA
  CCCAGAAGGGTCCCTCACAGTCCTGCCCGGGAGGTGGACAGGCTGGGTGTCATGACTCTGTTGCCAGCTT
  CCAGAGAAGAGGTACGAGACGACAAGACGACGATAAAATGTGAAACCTCCCCCCCATCCTCCCCAAGGCC
  TCTGCGCTTAGACAGGCTGCACAAGGGTGCGCTGCACACGGTCAGCCATGAGGACATCAGGGATCTGAGG
  AACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAACCCTAGCAGCAGCAATAGTAGCCAGGACTCCCTCC
  ACAAAGCCCCAAAGAAGAAGGGCATCAAATCCTCCATTGGCCGCTTATTTGGGAAGAAGGAAAAGGGCCG
  GCCTGGTCCGCTGGGCAAGGAGTCACCAGGGCAAGCTGGTGTTTCAGAGACAGAAAATTCATCTCAAGAT
  GCCTTGGGACTCAGCAAACTGGGAGGGCAAGCTGAGAAAAACCGGAAACTTCAGAAAAAGCATGAGCTGC
  TGGAGGAGGCCCGCAGGCAAGGCTTGCCTTTCGCACAGTGGGATGGGCCAACTGTGGTGGTGTGGCTAGA
  GCTCTGGGTTGGGATGCCTGCCTGGTATGTGGCTGCTTGCCGAGCAAACGTCAAGAGTGGTGCCATTATG
  TCAGCCCTGTCAGACACTGAGATCCAGCGGGAGATTGGCATCAGCAACCCCTTGCACAGGCTGAAGCTGC
  GGCTGGCTATCCAAGAGATAATGTCACTGACTAGCCCTTCTGCTCCACCCACATCTAGGACGACGCTAGC
  ATATGGGGACATGAACCACGAGTGGATTGGCAACGAGTGGCTGCCCAGCTTGGGCCTGCCGCAGTACCGC
  AGCTACTTCATGGAGTGCCTTGTCGATGCCCGGATGCTGGATCACCTGACCAAGAAAGACCTTCGAGGGC
  AGCTGAAGATGGTCGACAGCTTCCATAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGTTGAA
  TTATGATCGAAAAGAACTGGAAAGAAAACGAGAAGAGAGTCAGAATGAGACAAGAGATGTGCTTGTTTGG
  AGCAATGATCGGGTGATTCGCTGGATCCTGTCGATCGGCCTTAAAGAGTTTGCCAACAACCTAATAGAGA
  GCGGTGTTCACGGCGCACTTCTGGCCTTGGACGAGACGTTCGATTTCAGCGCCCTGGCACTCTTGTTACA
  GATTCCAACACAGAATACACAGGCTCGCGCTGTCTTGGAAAGAGAATTCAACAACCTTTTGGTCACCGGG
  ACTGATCGGAGGTTTGATGAGGATGATGACAAAAGCTTCAGGAGGGCACCTTCCTGGAGGAAGAAGTTCA
  GACCAAAGGACATCCGTGGCTTGGCTTCTGGCTCTGCAGAGACGTTGCCTGCCAACTTCCGGGTCACCTC
  CTCTATGTCATCTCCCTCTATGCAGCCAAAGAAGGTTCAGATGGACGTTTACCCACACTACTTCTACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210272 representing NM_001106320
  Red=Cloning site Green=Tags(s)
  
  MMCEVMPTISEAEGPPGGGGSHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHE
  VGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLR
  MTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQN
  SQKKALTEGMLDGNHEQENAPSTNGKRSSDGSLSHEDLAKVLELQEVIDRQAREQSQMKERLASLSSHAA
  ELEEDLDTARKDLIKSEEMNTKLQREVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIA
  NKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEA
  QLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVENAKKQLE
  ETQHDKDQLVLNIEALKAELEQMRLRGSSLHHGRPHLGSVPDFRFSVADGHVDAYSTSAVLRRPQKGRLA
  ALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDGEDDRDTLLSSVDLLSPSGQADAQTLAMM
  LQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLAGSSPPGSGRST
  PRRVPHSPAREVDRLGVMTLLPASREEVRDDKTTIKCETSPPSSPRPLRLDRLHKGALHTVSHEDIRDLR
  NSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGPLGKESPGQAGVSETENSSQD
  ALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIM
  SALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYR
  SYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNETRDVLVW
  SNDRVIRWILSIGLKEFANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVTG
  TDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLASGSAETLPANFRVTSSMSSPSMQPKKVQMDVYPHYFYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106320
• ORF Size: 3570 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106320.2, NP_001099790.2
• RefSeq Size: 4789 bp
• RefSeq ORF: 3573 bp
• Locus ID: 293645
• Cytogenetics: 1q42
• MW: 134.6 kDa