Chrna3 (NM_052805) Rat Tagged ORF Clone

CAT#: RR210212

Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)

Product Images

Circular map for RR210212

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210212 representing NM_052805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTTGTGCTGCTCCCGCCGCCGCTGTCCATGCTGATGCTGGTGCTGATGCTGCTGCCAGCGGCCA
  GTGCCTCAGAAGCTGAGCACCGCCTGTTCCAGTACCTGTTCGAAGATTACAACGAGATCATCCGGCCAGT
  GGCTAATGTGTCCCATCCAGTCATCATCCAGTTTGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAA
  GTAAACCAGATCATGGAAACCAACCTGTGGCTGAAGCAAATCTGGAATGACTACAAGCTGAAATGGAAAC
  CCTCTGACTACCAAGGGGTGGAGTTCATGCGTGTTCCTGCAGAGAAGATCTGGAAACCAGACATCGTACT
  GTACAACAACGCTGATGGGGATTTCCAGGTGGATGACAAGACCAAAGCTCTACTCAAGTACACAGGAGAA
  GTGACTTGGATCCCGCCGGCCATCTTTAAGAGCTCATGCAAAATCGACGTGACCTACTTCCCATTCGACT
  ACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGACCTGGTCCTCATCGG
  CTCCTCCATGAACCTCAAGGACTACTGGGAGAGTGGCGAGTGGGCTATCATTAAAGCCCCGGGCTACAAA
  CATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGCTGTACATCCGTCGCC
  TGCCGCTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTGCTTGTCTT
  CTACCTGCCCTCCGACTGTGGGGAGAAGGTGACACTCTGCATCTCTGTGCTCCTCTCCCTGACTGTCTTT
  CTCCTGGTGATCACCGAGACCATTCCTTCCACCTCGCTGGTCATCCCCCTGATTGGGGAGTACCTCCTCT
  TCACTATGATTTTTGTCACCTTGTCCATTGTCATCACAGTCTTTGTGCTCAATGTGCACTATAGAACTCC
  AACCACACACACCATGCCCACTTGGGTCAAGGCCGTGTTCTTGAACCTGCTCCCCAGGGTCATGTTTATG
  ACTAGGCCGACCAGTGGTGAGGGGGACACTCCTAAGACGAGGACCTTCTACGGCGCTGAGCTCTCAAACC
  TGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCAAGATGGGACCTGTGG
  CTACTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGAAGCTCCAGTTCTGAG
  TCTGTCGACGCTGTGTTGTCCCTCTCTGCCCTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTGAAGT
  ACATTGCCGAAAACATGAAAGCACAGAATGTAGCCAAAGAGATTCAAGATGATTGGAAGTACGTTGCCAT
  GGTGATTGATCGCATCTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACGGCGGGATTATTTCTG
  CAACCCTTGATGGCCAGAGATGACACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210212 representing NM_052805
  Red=Cloning site Green=Tags(s)
  
  MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDE
  VNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGE
  VTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYK
  HEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVF
  LLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFM
  TRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSE
  SVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFL
  QPLMARDDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052805
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052805.2, NP_434692.2
• RefSeq Size: 1500 bp
• RefSeq ORF: 1500 bp
• Locus ID: 25101
• Cytogenetics: 8q24
• MW: 57 kDa
• Gene Summary: subunit of nicotinic acetylcholine receptor that is specific to the brain and peripheral nervous system [RGD, Feb 2006]