Krt16 (NM_001008752) Rat Tagged ORF Clone

CAT#: RR210122

Krt16 (Myc-DDK-tagged ORF) - Rat keratin 16 (Krt16), (10 ug)

Product Images

Circular map for RR210122

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Krt16
• Synonyms: Ka16; Krt14; Krt14l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210122 representing NM_001008752
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTGCAGCCGTCAGTTCACCTCCTCCAGCTCCATGAAGAGCTCCTGTGGCATCGGTGGTGGCT
  CTAGCCGCATGTCCTCCGTCCTGGCTGGAGGATCCTGCCGGGCTCCCAGCACTTATGGGGGCATGTCGGT
  CACCTCCTCTCGCTTCTCCTCTGGGGCAGCCTGTGGGATTGGAGGTGGCTATAGTGGGGGCTTCAGCAGC
  AGCAGTTTTGGTGGTGGATTTGGTGGAGGACTTGGTGGTGGATTTGGTGGAGGACTTGGTGGTGGATTTG
  GTGGTGGTCTTGGCGATGGGCTCCTGGTTGGCAGTGAGAAAGTGACCATGCAAAACCTCAATGACCGCTT
  GGCCACCTACCTGGATAAGGTGCGTGCCCTGGAAGAGGCCAACAGTGACCTGGAGGTGAAGATCCGTGAC
  TGGTACCAGAGGCAGCGTCCCACTGAGATCAAAGACTACACCCCCTTCTTCAGGACCATCGAGGACCTGC
  AGAGCAAGATCGTTAGAGCCAAGCAGGAGAATGCGCAGTCCGTTTTGCAGATCGACAATGCCAGGCTGGC
  AGCTAATGACTTCAGGACCAAGTATGATAATGAGACGTCCTTGCGTCAGTTAGTGGAAAGTGACATCAAC
  AACCTTCGCCGTGTGCTGGATGAACTGACTATGTCCAGGGCAGACCTGGAGATGCAGATCGAGAGTCTCA
  GGGAGGAGCTGGCCTACCTGAAGAAGAATCACGAGGAGGAGATGCTTGCCTTGAGGGGTCAGACTGGTGG
  GGACGTCAACGTGGAGATGGACGCAGCTCCTGGTGTGGACCTGAGCCGCATCCTGAACGAGATGAGGGAC
  CAGTATGAGCAGATGGCAGAGAAGAACCGCAGAGATGTGGAGGCCTGGTTCCAGAGCAAGACCGAGGAGC
  TGAACCAAGAGGTGGCTTCTAACCATGAGCTGATACAGAGCGGCCGCAGCGAGGTGTCTGAGCTCCGCAG
  GGTGTTCCAGGGCCTGGAGATCGAACTGCAGTCCCAGCTCAGCATGAAAGCATCCTTGGAGAACAGCCTA
  GAAGAGACCAAAGGCAGATACTGCGTGCAGCTGTCCCAGATCCAGGGTTTGATCGGCAGCCTGGAGGAGC
  AGCTGGCTCAGCTGCGCTGCGAGATGGAGCAGCAGAGCCAGGAGTACAACATCTTGTTGGATGTGAAGAC
  TCGGCTGGAGCAGGAGATCGCCACCTACCGCCGTCTGCTGGATGGCGAGAATGTCCACAGCTCCTCCTCA
  CAGCACTCCTCTGGGCAGTCCTATTCTTCTGGAGAAGTCTTCTCCTCATCCTCCCGGCAGCCCCGGTCCA
  TCCTCAAGGAGCAAGGCTCAACCAGCTTCAGCCAGAGCCAAAGTCAGAGTTCCGGGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210122 representing NM_001008752
  Red=Cloning site Green=Tags(s)
  
  MATCSRQFTSSSSMKSSCGIGGGSSRMSSVLAGGSCRAPSTYGGMSVTSSRFSSGAACGIGGGYSGGFSS
  SSFGGGFGGGLGGGFGGGLGGGFGGGLGDGLLVGSEKVTMQNLNDRLATYLDKVRALEEANSDLEVKIRD
  WYQRQRPTEIKDYTPFFRTIEDLQSKIVRAKQENAQSVLQIDNARLAANDFRTKYDNETSLRQLVESDIN
  NLRRVLDELTMSRADLEMQIESLREELAYLKKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRD
  QYEQMAEKNRRDVEAWFQSKTEELNQEVASNHELIQSGRSEVSELRRVFQGLEIELQSQLSMKASLENSL
  EETKGRYCVQLSQIQGLIGSLEEQLAQLRCEMEQQSQEYNILLDVKTRLEQEIATYRRLLDGENVHSSSS
  QHSSGQSYSSGEVFSSSSRQPRSILKEQGSTSFSQSQSQSSGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008752
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008752.1, NP_001008752.1
• RefSeq Size: 1392 bp
• RefSeq ORF: 1392 bp
• Locus ID: 303530
• Cytogenetics: 10q31
• MW: 50.8 kDa
• Gene Summary: an intermediate filament protein; upregulated in squamous cell papillomas and carcinomas [RGD, Feb 2006]