Pex11b (NM_001025684) Rat Tagged ORF Clone

CAT#: RR210009

Pex11b (Myc-DDK-tagged ORF) - Rat peroxisomal biogenesis factor 11 beta (Pex11b), (10 ug)

Product Images

Circular map for RR210009

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pex11b
• Synonyms: MGC112929
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210009 representing NM_001025684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCTGGGTCCGCTTTAGTGCTCAGAGCCAAGCCCGGGAGCGGCTCTGTAGGGCCGCCCAATATG
  CCTGTTCCCTTCTCGGCCATGCTTTGCAGAGACATGGGGCTAGTCCTGAGTTACAGAAACAGATTCGACA
  ACTGGAGGGTCATCTGAGCCTCGGACGAAAGTTGCTCCGCCTGGGGAACTCAGCAGACGCCCTGGAGTCA
  GCCAAAAGAGCTGTGCACCTGTCAGATGTCGTTCTGAGATTCTGCATCACTGTTAGTCACCTCAACAGAG
  CCTTGTACTTTGCCTGTGACAATGTTCTGTGGGCTGGGAAGTCTGGACTAGCTCCCCGTGTAGACCAGGA
  GAAGTGGGCACAGCGTTCCTTCAGGTACTATCTCTTCTCCCTCATCATGAACCTGAGCCGGGATGCTTAT
  GAGATTCGCCTGTTGATGGAACAAGAGACTTCAGCTCACAGCAGGCGAATGAAGATTTCTGGAGTAGGAG
  TCTCAGGATTAGAGACTGGGGGACCTGGAGGACCAGGGACTCCAGGAGGATGTCTGCCACAACTGGCTTT
  GAAATTTCGGCTCCGAATCCTGCTACTGGCACGTGTCCTTCGAGATCACCCCCCTCTTCTGCTGGATGTG
  CTGAGAAATGCCTGTGACCTCTTCATCCCACTGGACAAACTAGGCCTCTGGCGTTGTGGCCCTGGGATTG
  TGGGGCTGTGTGGCCTCATATCCTCCGTTCTGTCTATTCTCACCCTAATCTGTCCCTGGCTACGACTCAA
  GCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210009 representing NM_001025684
  Red=Cloning site Green=Tags(s)
  
  MDAWVRFSAQSQARERLCRAAQYACSLLGHALQRHGASPELQKQIRQLEGHLSLGRKLLRLGNSADALES
  AKRAVHLSDVVLRFCITVSHLNRALYFACDNVLWAGKSGLAPRVDQEKWAQRSFRYYLFSLIMNLSRDAY
  EIRLLMEQETSAHSRRMKISGVGVSGLETGGPGGPGTPGGCLPQLALKFRLRILLLARVLRDHPPLLLDV
  LRNACDLFIPLDKLGLWRCGPGIVGLCGLISSVLSILTLICPWLRLKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025684
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025684.1, NP_001020855.1
• RefSeq Size: 1456 bp
• RefSeq ORF: 777 bp
• Locus ID: 310682
• Cytogenetics: 2q34
• MW: 28.6 kDa