Ccm2 (NM_001126275) Rat Tagged ORF Clone

CAT#: RR209978

Ccm2 (Myc-DDK-tagged ORF) - Rat cerebral cavernous malformation 2 (Ccm2), (10 ug)

Product Images

Circular map for RR209978

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209978 representing NM_001126275
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGAGGGCAAGAAGGGCAAGAAGCCTGGAATTGTGTCGCCGTTTAAACGAGTATTCCTAAAAG
  GTGAAAAGAGTAGAGATAAGAAAGCCCATGAGAAGGTGACAGAGAGGCGCCCTCTACACACTGTGGTGCT
  GGCCTTGCCTGAGCGAGTCGAGCCAGACAGACTGCTGAGCGACTATATTGAGAAGGAGGTGAAGTATTTA
  GGGCAGTTAACATCCATTCCTGGCTACCTGAACCCTTCCAGTAGGACGGAAATCCTGCATTTCATAGACA
  AGGCAAAGCGGTCCCACCAACTCCCTGGGCACCTGACTCAGGAGCATGATGCTGTGCTCAGTCTGTCCGC
  CTACAATGTCAAGCTGGCCTGGAGGGATGGGGAGGACATTATCCTCAGGGTGCCCATCCACGACATCGCT
  GCTGTCTCCTATGTCCGAGATGATGCTGCACACCTGGTGGTCCTGAAGACAGCCCAGGACCCAGGCATCT
  CTCCCAGCCAAAGTCTGTGTGCGGAAGGTTCTAGAGGCCTCAGCGCAGGCTCCTTGTCAGAAAGTGCAGT
  GGGGCCAGTAGAGGCCTGTTGCCTGGTCATCATGGCCACAGAGAGCAAGGTCGCCGCTGAAGAACTGTGC
  TCCCTGCTCAGCCAGGTCTTCCAGATCGTCTACACAGAGTCCACCATCGACTTTCTGGACCGAGCAATAT
  TCGACGGGGCCTCCACCCCTACCCACCACCTGTCAGTGCACAGCGATGACTCTTCCACGAAAGTGGACAT
  GAAGGACAGTTATGACGCCGACGCCAGCACCTTCTGCTTCCCGGACTCTGGGGATGTGGGGGGCTTGCCG
  CCCTTGCCCTTCTGCATGCAGGCATCCCCCCACAGCAAGACGGTCAGCGAGAGTGAGCTGAGTACCAGCG
  CCACGGAGCTGCTGCAGGACTACATGCTCACGTTACGTACCAAGCTGTCATCACAGGAGATCCAGCAGTT
  TGCAGCCCTGCTGCATGAATACCGCAACGGGGCCTCCATCCATGAGTTCTGCATCAGCCTGCGGCAGCTC
  TATGGAGACAGCCGCAAGTTCCTGCTGCTTGGTCTGAGGCCCTTTATACCTGAGAAGGACAGTCAGCACT
  TTGAAAACTTCCTGGAGACCATTGGCGTGAAAGACGGCCGTGGCATCATCACTGACAGCTTTGGCAGGCA
  TCGCCGTGCTCTGAGCACCACCTCCACATCCACCACCAATGGGAACAGGACCACAGGCAGCCCTGATGAC
  CGCTCAGCGCCCTCAGAGGGGGATGAGTGGGACCGCATGATCTCAGACATCAGCAGTGATATTGAAGCAC
  TAGGCTGCAGCATGGACCAGGACTCAGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209978 representing NM_001126275
  Red=Cloning site Green=Tags(s)
  
  MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLALPERVEPDRLLSDYIEKEVKYL
  GQLTSIPGYLNPSSRTEILHFIDKAKRSHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIA
  AVSYVRDDAAHLVVLKTAQDPGISPSQSLCAEGSRGLSAGSLSESAVGPVEACCLVIMATESKVAAEELC
  SLLSQVFQIVYTESTIDFLDRAIFDGASTPTHHLSVHSDDSSTKVDMKDSYDADASTFCFPDSGDVGGLP
  PLPFCMQASPHSKTVSESELSTSATELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCISLRQL
  YGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSTSTTNGNRTTGSPDD
  RSAPSEGDEWDRMISDISSDIEALGCSMDQDSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126275
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126275.1, NP_001119747.1
• RefSeq Size: 1807 bp
• RefSeq ORF: 1362 bp
• Locus ID: 305505
• Cytogenetics: 14q21
• MW: 49.8 kDa