Dll1 (NM_032063) Rat Tagged ORF Clone

CAT#: RR209633

Dll1 (Myc-DDK-tagged ORF) - Rat delta-like 1 (Drosophila) (Dll1), (10 ug)

Product Images

Circular map for RR209633

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dll1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209633 representing NM_032063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGTCGGAGCGCGCTAGCCCTTGCCGTGGTCTCAGCCCTGCTGTGCCAGGTCTGGAGCTCTGGCG
  TATTTGAGCTGAAGCTGCAGGAGTTCGTCAACAAGAAGGGGCTGCTGGGGAACCGCAACTGCTGCCGCGG
  GGGCTCTGGCCCGCCGTGCGCCTGCAGGACCTTCTTTCGCGTATGCCTCAAGCATTACCAGGCCAGCGTG
  TCCCCGGAGCCACCCTGCACCTACGGCAGTGCGGTCACCGCAGTGCTGGGTGTCGACTCCTTCAGCCTGC
  CTGATGGCGCAGGCATCGACCCCGCCTTCAGCAACCCCATCCGATTCCCCTTCGGATTCACCTGGCCAGG
  TACCTTCTCTCTGATCATTGAAGCCCTCCACACAGATTCTCCTGACGACCTCGCAACAGAAAACCCAGAA
  AGACTCATCAGCCGCCTGACCACACAGAGGCACCTCACTGTGGGAGAAGAGTGGTCTCAGGACCTTCACA
  GTAGCGGCCGCACAGACCTCCGCTACTCTTACCGGTTTGTGTGTGATGAACACTACTATGGAGAAGGCTG
  CTCCGTGTTCTGCCGACCGCGGGATGATGCCTTTGGCCACTTCACCTGCGGGGAGAGAGGGGAGAAGATG
  TGCGACCCTGGCTGGAAAGGCCAGTACTGCACTGACCCCATTTGTCTGCCAGGCTGTGATGACCAACATG
  GATATTGTGACAAACCGGGGGAATGCAAGTGCAGAGTTGGCTGGCAGGGCCGCTACTGCGATGAATGCAT
  CCGATACCCAGGCTGTCTCCATGGTACCTGCCAGCAGCCCTGGCAGTGTAACTGCCAGGAAGGCTGGGGG
  GGCCTCTTCTGCAACCAGGATCTGAACTACTGCACTCACCATAAGCCATGCAGGAACGGAGCCACCTGCA
  CCAACACGGGCCAGGGGAGCTACACATGCTCTTGCCGACCCGGGTATACAGGGGCCAACTGTGAGCTGGA
  GGTAGATGAGTGTGCTCCCAGCCCCTGCAGGAATGGAGGGAGCTGCACGGATCTTGAGGACAGCTACTCT
  TGCACCTGCCCTCCTGGCTTCTATGGCAAGGTCTGTGAGCTGAGCGCCATGACGTGTGCAGATGGTCCTT
  GCTTCAATGGGGGACGATGTTCGGATAACCCCGATGGAGGCTACACCTGCCATTGCCCTGCGGGCTTCTC
  TGGCTTCAACTGTGAGAAGAAGATTGATCTCTGTAGCTCTTCCCCTTGTTCTAACGGTGCCAAGTGTGTG
  GACCTCGGCAACTCCTACCTGTGCCGATGTCAGACTGGCTTCTCCGGGAGGTACTGCGAGGACAATGTGG
  ATGACTGTGCCTCTTCTCCCTGTGCAAACGGGGGCACCTGCCGGGACAGTGTGAACGATTTCTCCTGTAC
  CTGCCCACCTGGCTACACAGGCAGGAACTGCAGCGCCCCTGTCAGCAGGTGTGAGCATGCACCCTGTCAT
  AACGGGGCCACCTGCCACCAGAGGGGCCAACGCTACATGTGTGAGTGCGCCCAGGGCTATGGCGGCGCCA
  ACTGCCAGTTCCTGCTCCCTGAGCCACCACCAGACCTCATAGTGGCGGCCCAGGGCGGGTCCTTCCCCTG
  GGTGGCTGTGTGTGCCGGGGTGGTGCTTGTCCTCCTGCTGCTGCTGGGCTGTGCTGCTGTGGTGGTCTGC
  GTCCGGCTGAAGCTACAGAAACACCAGCCTCCGCCTGATCCTTGCGGGGGAGAGACAGAGACCATGAACA
  ACCTAGCCAATTGCCAGCGTGAGAAGGATGTTTCTGTTAGCATCATTGGGGCTACACAGATCAAGAACAC
  CAACAAGAAGGCGGACTTTCATGGGGACCATGGTGCTGACAAGAGCAGCTTTAAGGCCCGATACCCCACT
  GTGGACTATAACCTCATTCGAGACCTCAAGGGAGATGAAGCCACGGTCAGGGATGCACACAGCAAACGTG
  ACACCAAGTGCCAGTCACAGGGCTCTGTAGGAGAAGAGAAGAGCACCTCAACGCTCAGGGGTGGGGAGGT
  TCCCGACAGAAAAAGGCCCGAGTCTGTCTACTCTACTTCAAAGGACACCAAGTACCAGTCGGTGTATGTT
  CTATCTGCAGAAAAGGATGAGTGTGTTATAGCGACTGAGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209633 representing NM_032063
  Red=Cloning site Green=Tags(s)
  
  MGRRSALALAVVSALLCQVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASV
  SPEPPCTYGSAVTAVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPE
  RLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKM
  CDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWG
  GLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCRNGGSCTDLEDSYS
  CTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPAGFSGFNCEKKIDLCSSSPCSNGAKCV
  DLGNSYLCRCQTGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGRNCSAPVSRCEHAPCH
  NGATCHQRGQRYMCECAQGYGGANCQFLLPEPPPDLIVAAQGGSFPWVAVCAGVVLVLLLLLGCAAVVVC
  VRLKLQKHQPPPDPCGGETETMNNLANCQREKDVSVSIIGATQIKNTNKKADFHGDHGADKSSFKARYPT
  VDYNLIRDLKGDEATVRDAHSKRDTKCQSQGSVGEEKSTSTLRGGEVPDRKRPESVYSTSKDTKYQSVYV
  LSAEKDECVIATEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032063
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032063.2, NP_114452.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 2145 bp
• Locus ID: 84010
• UniProt ID: P97677
• Cytogenetics: 1q12
• MW: 77.4 kDa
• Gene Summary: homolog of the Notch Delta ligand; member of the delta/serrate/jagged family; may play a role in cell fate decisions during hematopoiesis [RGD, Feb 2006]