Cul4a (NM_001127301) Rat Tagged ORF Clone

CAT#: RR208903

Cul4a (Myc-DDK-tagged ORF) - Rat similar to cullin 4A (RGD1563853), (10 ug)

Product Images

Circular map for RR208903

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cul4a
• Synonyms: RGD1563853
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208903 representing NM_001127301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAGGGCCCTCGGAAGGGCAGCGTCTCGGCTCTCATGGGCCGCACCAACGGCCTCACCAAGC
  CCGCGGCGTTGGCCAGTGGCCCCGCCAAACCGGGAGGCACGGGCGGCTCCAGGAAGCTGGTCATCAAGAA
  CTTCCGAGACAGGCCGCGGCTGCCTGACAACTACACTCAGGACACGTGGCGGAAGCTTCACGAAGCGGTC
  AAGGCCATCCAGAGCAGCACATCCATCAGGTACAACCTGGAGGAACTGTACCAGGCTGTTGAGAATCTGT
  GCTCTCACAAAGTCTCCCCCACGCTCTACAAGCAGCTGCGGCAGGTCTGTGAAGACCATGTCCAGGCCCA
  GATCCTCCCCTTCAGAGAAGACTCACTAGACAGCGTTTTGTTTCTGAAGAAGATTAACACATGCTGGCAA
  GATCACTGCAGACAAATGATCATGATCAGAAGCATCTTCCTGTTTCTGGACCGCACCTACGTCCTCCAGA
  ACTCCATGCTGCCTTCCATCTGGGACATGGGCCTAGAGCTGTTTAGGAACCATATCATCAGTGACAGGAT
  GGTGCAAAGCAAGACCATTGATGGGATCCTGCTGCTGATTGGGCGTGAACGAAGTGGTGAGGCTGTGGAC
  AGGAGTCTGCTTCGCAGTCTGTTGAGCATGCTCTCAGATCTCCAGGTCTACAAAGACTCATTCGAACTGA
  AGTTCTTGGAAGAGACTAACTGTCTGTACGCTGCTGAAGGCCAGCGGCTGATGCAAGACAGGGAGGTTCC
  AGAATACCTTAACCATGTGAGTAAACGTCTAGAAGAGGAAGCAGACCGAGTGATCACTTACTTAGACCAC
  AGCACACAAAAACCGTTGATTGCCTGTGTGGAGAAACAGTTGTTAGGAGAACATTTGACAGCAATTCTTC
  AGAAAGGGCTTGAGCAGCTGCTGGATGAGAACAGGGTGCCCGACCTCACACAGATGTACCAGCTCTTCAG
  CCGGGTGAAAGGGGGCCAGCACGCACTGCTGCAGCACTGGAGCGAGTACATCAAGACCTTTGGGACAACC
  ATTGTCATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGCTGGACTTTAAGGACAAAGTGGACC
  ACGTGGTGGAGGTGTGCTTCCAGAGGAACGAGCGCTTCATCAACCTGATGAAGGAGTCCTTTGAGACGTT
  CATAAACAAGAGACCAAACAAGCCGGCAGAGCTCATTGCCAAGCACGTGGATTCAAAGTTACGAGCAGGC
  AACAAGGAAGCAACAGACGAGGAGCTGGAGAGGATCCTGGACAAGATCATGATTCTGTTCCGATTCATCC
  ACGGTAAAGATGTCTTTGAAGCATTTTATAAGAAAGACTTAGCAAAAAGGCTGCTAGTGGGGAAGAGCGC
  GTCGGTGGACGCGGAGAAGTCCATGCTGTCCAAGCTGAAGCATGAGTGTGGAGCAGCTTTTACCAGCAAG
  CTGGAGGGCATGTTCAAGGACATGGAGCTGTCCAAAGACATCATGGTTCACTTCAAGCAGCATATGCAGA
  ACCAAAGTGCACCAGGCCCCATCGACCTCACGGTGAACATCCTCACCATGGGCTACTGGCCTACATACAC
  ACCCATGGAAGTGCACCTCCCTCCAGAGATGGTCAGACTTCAGGAAGTATTCAAGACGTTCTACCTGGGC
  AAGCACAGTGGCCGGAAGCTGCAGTGGCAGACGACGCTGGGACACGCTGTGTTGAAGGCGGACTTTAAGG
  AGGGGAAGAAGGAGTTCCAGGTGTCCCTGTTCCAGACGCTCGTGCTGCTCATGTTCAACGAGGGCGACGG
  GTTCAGCTTCGAAGAAATCAAAATGGCCACCGGCATAGAGGACAGTGAGTTGCGAAGAACACTGCAGTCT
  CTCGCCTGTGGTAAAGCCCGGGTCCTGATTAAAAGTCCCAAAGGGAAGGAGGTGGAAGACGGAGATAAGT
  TCATCTTCAATGCAGACTTCAAACACAAGTTGTTCAGAATAAAGATCAATCAAATCCAGATGAAGGAAAC
  CGTTGAAGAACAGGTTAGCACCACAGAGAGAGTGTTCCAGGACAGACAGTACCAGATCGACGCTGCTATA
  GTCAGAATAATGAAAATGAGGAAGACACTTGGTCATAATCTTCTAGTTTCTGAATTATACAATCAGCTGA
  AATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGGATTGAGTCACTTATAGACAGAGACTATATGGAACG
  AGACAAAGACAGTCCAAATCAGTACCACTACGTGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208903 representing NM_001127301
  Red=Cloning site Green=Tags(s)
  
  MADEGPRKGSVSALMGRTNGLTKPAALASGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAV
  KAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLRQVCEDHVQAQILPFREDSLDSVLFLKKINTCWQ
  DHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVD
  RSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDH
  STQKPLIACVEKQLLGEHLTAILQKGLEQLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTT
  IVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAG
  NKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSK
  LEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLG
  KHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQS
  LACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAI
  VRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127301
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127301.1, NP_001120773.1
• RefSeq Size: 2859 bp
• RefSeq ORF: 2280 bp
• Locus ID: 361181
• Cytogenetics: 16q12.5
• MW: 87.8 kDa