Sema7a (NM_001108153) Rat Tagged ORF Clone

CAT#: RR208439

Sema7a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), (10 ug)

Product Images

Circular map for RR208439

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema7a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208439 representing NM_001108153
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCCTCCTCCTCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCTCAGCCTGCCGGCTC
  GGTTCGGGCTCCCGCTGCGGCTGCGGCTGCTGCTGGTGTTCTGGGTGACCGCCGCCTCCGCCCAAGGCCA
  CTCGAGGAGCGGACCCCGTATCTCCGCCGTCTGGAAAGGGCAGGACCATGTGGACTTTGGCCAGCCTGAG
  CCACACAATGTGCTTTTCCATGAGCCGGGCAGCTTCTCTGTCTGGGTAGGTGGACGAGGCAAGGTCTACC
  TCTTCAACTTCCCCGAGGGCAAGAATGCCTCTGTGCGCACGGTGAACATTGGCTCCACAAAAGGGTCCTG
  TCAGGACAAACAGGACTGCGAGAATTACATCACTCTTCTTGAGAGGCGGGGTAATGGACTGCTGGTCTGT
  GGCACCAATGCCCGGAAACCCAGCTGCTGGAACTTGGTGAATGACAGTGTGGTGATGTCACTTGGTGAGA
  TGAAAGGCTATGCCCCCTTCAGCCCGGATGAGAACTCCCTGGTTCTGTTTGAAGGGGATGAAGTGTACTC
  TACAATCCGGAAGCAGGAATACAATGGCAAGATCCCTCGCTTTCGCCGCATTCGGGGAGAGAGTGAACTG
  TACACAAGTGATACTGTCATGCAGAATCCACAGTTCATCAAGGCCACCATTGTGCACCAGGACCAAGCCT
  ATGATGATAAGATCTACTACTTCTTCCGAGAAGACAACCCTGACAAGAACCCCGAGGCTCCGCTCAATGT
  ATCCCGAGTGGCCCAGTTATGCAGGGGGGACCAGGGTGGTGAGAGTTCATTGTCTGTCTCCAAGTGGAAC
  ACCTTCCTGAAAGCCATGCTGGTTTGCAGCGACGCAGCCACCAACAGGAACTTCAATCGACTGCAAGATG
  TCTTCCTACTCCCTGACCCCAGTGGCCAGTGGAGGGATACCAGGGTCTATGGCGTTTTCTCCAACCCCTG
  GAACTACTCAGCTGTCTGCGTGTATTCGCTTGGTGACATTGACAGAGTCTTCCGTACCTCCTCACTCAAA
  GGCTACCACATGGGCCTTCCCAACCCTCGACCTGGCATGTGCCTCCCAAAAAAGCAACCCATACCCACGG
  AAACCTTCCAGGTAGCCGACAGTCACCCGGAGGTGGCCCAGAGAGTGGAACCCATGGGGCCACTGAAGAC
  ACCATTGTTCCACTCTAAGTACCATTACCAGAAAGTGGTTGTCCACCGCATGCAAGCCAGCAATGGGGAG
  ACCTTCCATGTACTTTACCTAACCACAGACAGGGGCACCATTCACAAGGTGGTGGAATCAGGGGACCAGG
  AACACAGCTTTGTCTTCAACATCATGGAGATCCAGCCCTTTCACCGTGCAGCTGCCATCCAGGCCATATC
  GTTGGATGCTGACCGGCGGAAGCTCTATGTGACCTCCCCGTGGGAAGTGAGCCAGGTACCCCTGGACATG
  TGTGGGGTCTACAGTGGGGGCTGCCATGGCTGCCTCATGTCCCGAGACCCCTACTGTGGCTGGGACCAGG
  ACCAGGACCGCTGTGTGTCTATCTACAGCTCCCAACGGTCAGTGCTGCAGTCCATCAATCCAGCTGAGCC
  ACACAGAGAGTGTCCCAACCCCAACCCAGATGAGGCTCCATTGCAGAAGGTTTCCCTGGCCCGGAACTCT
  CGATACTACCTGACCTGCCCCATGGAGTCCCGCCACGCCACCTACTTATGGCGCCACGAGGAGAATGTGG
  AACAGAGCTGTGAGCCAGGCCACCAAAGCCCCAGCTGCATTCTATTCATAGAGAACCTCACTGCCCGTCA
  GTACGGCCACTACCGCTGCGAGGCCCAAGAGGGTTCCTACCTCCATGAGGCTCAACACTGGGAGCTGCTG
  CCGGAGGACAGAGCGCTGGCCGAACAGCTCTTGGGCCATGCCCGGGCCCTGGCCGCCTCCTTCTGGCTGG
  GGGTCCTGCCCACACTCGTACTAGGTCTGCTGGTTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208439 representing NM_001108153
  Red=Cloning site Green=Tags(s)
  
  MTPPPPGRAAPSAPRARVLSLPARFGLPLRLRLLLVFWVTAASAQGHSRSGPRISAVWKGQDHVDFGQPE
  PHNVLFHEPGSFSVWVGGRGKVYLFNFPEGKNASVRTVNIGSTKGSCQDKQDCENYITLLERRGNGLLVC
  GTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESEL
  YTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWN
  TFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLK
  GYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGE
  TFHVLYLTTDRGTIHKVVESGDQEHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSPWEVSQVPLDM
  CGVYSGGCHGCLMSRDPYCGWDQDQDRCVSIYSSQRSVLQSINPAEPHRECPNPNPDEAPLQKVSLARNS
  RYYLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLHEAQHWELL
  PEDRALAEQLLGHARALAASFWLGVLPTLVLGLLVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108153
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108153.1, NP_001101623.1
• RefSeq Size: 2622 bp
• RefSeq ORF: 2001 bp
• Locus ID: 315711
• Cytogenetics: 8q24
• MW: 75.1 kDa