Dbn1 (NM_031024) Rat Tagged ORF Clone

CAT#: RR208356

Dbn1 (Myc-DDK-tagged ORF) - Rat drebrin 1 (Dbn1), (10 ug)

Product Images

Circular map for RR208356

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dbn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208356 representing NM_031024
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGTCAGCTTCAGCGGCCACCGCCTGGAGCTGCTGGCGGCGTACGAGGAGGTGATCCGGGAGG
  AGAGTGCAGCCGACTGGGCTCTGTACACTTACGAAGATGGCTCAGATGACCTCAAGCTTGCAGCATCAGG
  AGAAGGAGGCTTGCAGGAGCTTTCAGGCCACTTCGAGAACCAGAAAGTGATGTACGGTTTCTGCAGCGTC
  AAGGACTCCCAGGCTGCCTTGCCAAAATATGTGCTCATCAACTGGGTTGGTGAGGATGTGCCTGATGCCC
  GAAAATGTGCTTGTGCCAGCCATGTGGCCAAGGTGGCTGAATTCTTCCAGGGCGTTGACGTAATTGTGAA
  TGCCAGCAGTGTGGAGGATATCGATGCTGGCGCCATTGGGCAGCGGCTCTCCAATGGACTGGCTCGGCTC
  TCTAGCCCAGTGCTGCACCGCCTGCGCCTTCGGGAGGATGAGAATGCTGAGCCGGTGGGCACCACCTACC
  AGAAGACGGATGCGGCCGTGGAGATGAAGCGGATTAACCGTGAGCAGTTTTGGGAGCAGGCCAAGAAGGA
  GGAGGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCTTTGGATGCCAGGCTCAGGTTTGAGCAGGAGCGG
  ATGGAGCAGGAGCGGCAGGAGCAGGAGGAACGGGAACGGCGCTACCGAGAGCGGGAGCAGCAGATCGAGG
  AGCACAGGAGGAAACAGCAGAGTCTGGAAGCTGAAGAGGCCAAGAGGAGGTTAAAGGATCAGTCTATCTT
  TGGTGATCAGCGAGATGAAGAGGAAGAGTCCCAGATGAAGAAGTCGGAATCTGAGGTGGAGGAGGCAGCT
  GCCATCATTGCCCAGCGGCCTGATAACCCACGGGAGTTCTTCAGACAGCAGGAACGAGTGGCTTCAGCCT
  CTGGTGGCAGCTGTGACGCACCCTCGCCCTTCAACCACCGACCAGGTCGTCCGTACTGCCCTTTCATAAA
  GGCATCGGACAGTGGGCCTTCCTCCTCCTCCTCTTCCTCCTCTTCCCCTCCACGGACTCCCTTTCCCTAT
  ATCACCTGCCACCGCACCCCAAACCTCTCTTCCTCCCTCCCATGCAGTCACCTGGACAGCCACCGGAGGA
  TGGCGCCCACTCCCATTCCCACCCGGAGCCCATCTGATTCCAGCACAGCCTCCACCCCCATCACGGAGCA
  GATCGAGAGGGCCCTGGATGAGGTCACATCCTCGCAGCCTCCACCCCCACCTCCACCACCCCCACCAGCT
  CAAGAGGCCCAGGAGTCTGCTCCCAGACTGGACGGTGAAGAGGTCTGCAAGGAGGCCAAAGTAGCAGCAG
  CTCCTCAGGTCTGGGCTGGCTGTGCAGAGGAGCCTCCTCGGGCACAGGAACCTCCCCTGTTGCAAAGCAG
  CCCCACGGAGGACTTGATGTGCACAGAATCTCCTGAGCAGGCTGTCCTGGCGGCCTCTCCAGAGCCTGAT
  GCCTCTGTCACCTCAGTAGCTGATGCTCATGCAGCTGACACCATCGAGACCACCACTGCCACTACTGCCA
  CCACTATTGCCGACAACGTCACCCCTGCCGCCGCCAGCCTCATTGATCTGTGGCCTGGTAACGGGGAAGA
  GGCCTCAACGCCTCAGGCTGAACCCAGGGTGCCCACCCCACCCTCAGGTGCTGAGGCCTCCCTGGCAGAG
  GTGCCTCTGTTGAATGAGGCAGCTCAGGAGCCGCTGCCACCAGTGGGCGAAGGCTGTGCCAACCTTCTTA
  ATTTCGATGAGCTGCCAGAACCTCCAGCCACCTTCTGTGACCCAGAGGAGGAAGCCGAAGGAGAGCCGCT
  GGCTGCCTCCCAGGTCCTAACTATGCCCTCAGCCCTAGAGGAGGTAGATCAGGTGTTGGAGCAGGAGTTG
  GAGCCAGAACCTCATCTGCTGACCAATGGAGAGACCACTCAGAAGGAGGGGACCCAGCAGGCCAGCGAAG
  GATACTTCAGTCAGTCACAGGAGGAAGAATTCGCCCAATCAGAAGAGCCATGTGCAAAGGCTCCACCTCC
  TGTATTCTACAACAAGCCTCCAGAAATCGACATCACCTGCTGGGATGCAGACCCAGTTCCTGAAGAGGAA
  GAGGGCTTCGAGGGTGGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208356 representing NM_031024
  Red=Cloning site Green=Tags(s)
  
  MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSV
  KDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARL
  SSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDARLRFEQER
  MEQERQEQEERERRYREREQQIEEHRRKQQSLEAEEAKRRLKDQSIFGDQRDEEEESQMKKSESEVEEAA
  AIIAQRPDNPREFFRQQERVASASGGSCDAPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPY
  ITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPITEQIERALDEVTSSQPPPPPPPPPPA
  QEAQESAPRLDGEEVCKEAKVAAAPQVWAGCAEEPPRAQEPPLLQSSPTEDLMCTESPEQAVLAASPEPD
  ASVTSVADAHAADTIETTTATTATTIADNVTPAAASLIDLWPGNGEEASTPQAEPRVPTPPSGAEASLAE
  VPLLNEAAQEPLPPVGEGCANLLNFDELPEPPATFCDPEEEAEGEPLAASQVLTMPSALEEVDQVLEQEL
  EPEPHLLTNGETTQKEGTQQASEGYFSQSQEEEFAQSEEPCAKAPPPVFYNKPPEIDITCWDADPVPEEE
  EGFEGGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031024
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031024.1, NP_112286.1
• RefSeq Size: 2697 bp
• RefSeq ORF: 2124 bp
• Locus ID: 81653
• UniProt ID: Q07266
• Cytogenetics: 17p14
• MW: 77.5 kDa
• Gene Summary: may play a role in neurite outgrowth [RGD, Feb 2006]