Tank (NM_145788) Rat Tagged ORF Clone

CAT#: RR207981

Tank (Myc-DDK-tagged ORF) - Rat TRAF family member-associated NFKB activator (Tank), (10 ug)

Product Images

Circular map for RR207981

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tank
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207981 representing NM_145788
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAAACATTGGCGAGCAACTCAATAGAGCATATGAAGCCTTCCGACAGGCATGCATGGATAGAG
  ATTCAGCAGTAAGAGAGCTGCAGCAGAAGCAGACTGAGAACTATGAACAAAGAATACGCGAGCAACAGGA
  ACAGCTGTCATTTCAACAAACTCTCATTGACAGGCTAAAGTCACAGCTACGTCTTGTGGATTCTAGTCGA
  GATAACACTTGTGACTATGTACCTTTGCTTGAAGACAGTGACAGAAGGAAGAATAATTTGACCCTTGATG
  AACCACATGATAAAGTGAAACTAGGAACACTGAGAGATAAGCAATCAAAGGTGAGAAGACAAGAAGTTTC
  TTCTGGAAAAGAGACCTCAAAGGATCTCAGCATCCCTTTGCATCATGAAAGGGATAATATAGAGAAGACT
  TTCTGGGACCTTAAAGAAGAATTTCATAGGATATGCTTACTAGCAAAAGCACAGAAAGACCACTTAAGCA
  AACTTAATATACCAGATATTGCAACTGACACACAGTGTTCTGTGCCTATACAGTGTACCGATAAAACAGA
  AAAACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATGATATAAATAGAGGTATGTCGTGCGTCACATCT
  GTCACACCAAGAGGACTGGGCCGGGATGAGGAAGATACCTCTTTTGAATCGCTTTCTAAATTCAATGTCA
  AGTTTCCGCCTGTGGACAGTGACTCTACTTTTCTACATAGCACTCCAGAGACCCCGAGCATCCTTGCTCC
  CTCCACACCTGAGGCAGTGTGCCAGGACAAGTTTAATGTGGAAGTCAGAGACAGCCCAGGAAACTTGGTT
  AAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTAACCCCATAACTTCAGCTATACAAAACCTTAAAA
  CAACTGACAAAACAAAACCCTCAAATCTTAGAGCTCCGTGTTTGCCAGCTGGAGACCATAATGTGTTCTA
  TGTAAATACGTTCCCACTTCAAGACCCGCCTGACGCACCTTTTCCCTCACTGGATTCCCCAGGAAAGGCT
  GTCCGAGGACCACAGCAGCCCCTTTGGAAGCCTTTTCCTAACCAAGACACTGACTTAGTGGTACCAAGTG
  GTTCAGACTCAGTACTCCTTAAACCTCAAGTGTGTGAGTTCTGTCAAGAGCTTTTCCCACCATCCCTTAC
  ATCCAGAGGGGATTTCCTCCGGCATCTTAATACACACTTTAATGGAGAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207981 representing NM_145788
  Red=Cloning site Green=Tags(s)
  
  MDKNIGEQLNRAYEAFRQACMDRDSAVRELQQKQTENYEQRIREQQEQLSFQQTLIDRLKSQLRLVDSSR
  DNTCDYVPLLEDSDRRKNNLTLDEPHDKVKLGTLRDKQSKVRRQEVSSGKETSKDLSIPLHHERDNIEKT
  FWDLKEEFHRICLLAKAQKDHLSKLNIPDIATDTQCSVPIQCTDKTEKQEALFKPQAKDDINRGMSCVTS
  VTPRGLGRDEEDTSFESLSKFNVKFPPVDSDSTFLHSTPETPSILAPSTPEAVCQDKFNVEVRDSPGNLV
  KTEETLFEIQGINPITSAIQNLKTTDKTKPSNLRAPCLPAGDHNVFYVNTFPLQDPPDAPFPSLDSPGKA
  VRGPQQPLWKPFPNQDTDLVVPSGSDSVLLKPQVCEFCQELFPPSLTSRGDFLRHLNTHFNGET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145788
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145788.2, NP_665731.2
• RefSeq Size: 2077 bp
• RefSeq ORF: 1245 bp
• Locus ID: 252961
• Cytogenetics: 3q21
• MW: 47 kDa