Fbxl5 (NM_001107222) Rat Tagged ORF Clone

CAT#: RR207622

Fbxl5 (Myc-DDK-tagged ORF) - Rat F-box and leucine-rich repeat protein 5 (Fbxl5), (10 ug)

Product Images

Circular map for RR207622

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxl5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207622 representing NM_001107222
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGCTGGTGGGGCGCTACTGCGACAAGCTTTCTAAAACCAACTTTTCCAACAACAATGACTTCC
  GTGCTCTTCTCCAGTCTTTATATGCTACTTTCAAGGAGTTTAAAATGCATGAACAAATTGAAAATGAATA
  CATTATTGGTTTACTTCAGCAACGCAGTCAGACCATTTACAATGTGCATTCTGACAATAAGCTCTCCGAG
  ATGCTTAGTCTCTTTGAAAAGGGGCTGAAGAATGTTAAGAATGAGTGTGAACAGTTGAATTATGCAAAGC
  AGCTGAAGGAGAGATTGGAGGCCTTCACGAGAGATTTCCTTCCCCACATGAAAGAGGAGGAGGAGGTTTT
  TCAGCCCATGTTAATGGAATATTTTACCTATGAAGAGCTTAAAGATATCAAAAAGAAAGTGATCGCACAA
  CACTGCTCACAGAAGGACACAGCAGAACTCCTCAGAGGGCTCAGTCTGTGGAATCAAGCTGAAGAGCGAC
  AGAAAGTTTTCAAATATTCTGTGGATGAGAAGTCAGATGCAGAAGCGGAAGTGTCAGAACACTCCACAGG
  TATAACCCATCTTCCTCCTGAGGTAATGCTGTCCATTTTCAGTTACCTTAATCCTCAAGAATTGTGTCGG
  TGCAGTCAAGTCAGCACTAAGTGGTCTCAGCTGGCAAAGACAGGATCTTTGTGGAAACATCTTTACCCTG
  TTCATTGGGCAAGAGGTGACTGGTATAGTGGCCCTGCCACTGAACTTGATACTGAACCTGATGAGGAATG
  GGTGAGAAGTAGGAAAGATGAAAGTCGTGCTTTCCAGGAATGGGACGAAGATGCCGATATAGATGAATCT
  GAAGAGTCTGCAGAGGAATCGGTTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTAA
  TTCATAATGTTCTGCCATATGTTGGTACCTCTGTAAAAACTTTAGTGTTAGCATACAGCTCTGCAGTCTC
  CAGCAAAATGAGTCTTCGGCATCTTGACTTGTCTGGATGTGAAAAAATCACAGACATGGCTCTGGAGAAG
  ATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCGTTCTGAAAAGTGCAGGCAAGACTACTT
  CAACTCCATGGACAAATAAAGACATTACCATGCCATCCACCACGCAGTATGCCTGTTTGCACAATTTAAC
  TAACAAAGGCGTTGGTGAAGAAATCGACAGCGAACACACTTGGACTGAACCTGTGTCTTCTGAAAGTTTC
  ACTCCTCCCTACGTGTGGATGCTAGATGCTGAAGATTTGGCCGATATCGAAGATGCTGTAGAATGGAGGC
  ACAGAAACGTTGAAAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTTGTTCCTCCTCTGGTTGTTA
  CAGCAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCTCCAGCCTTTGCG
  TATTGTGGTCATTCATTCTGTTGCACAGGGACAGCTCTAAGAACTATGTCTGCACTCCCAGCGGCTTCTG
  CAGTGTGTAGAAAAGCATTAAGGACTACGTTGCCCAGGGGGAAAGACTTAATTTATTTTGGGAGTGAAAA
  GTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATGTTACCAGATCACAGACCAT
  GGTCTCAGGGTTCTGACTCTGGGAGGAGGACTGCCTTACTTGGAGCACCTGAATCTCTCTGGCTGTCTGA
  CTGTAACTGGTGCAGGACTGCAGGATTTGGTCTCAGCATGTCCTTCCCTAAATGACGAATACTTCTACTA
  CTGTGACAACATTAACGGTCCTCATGCTGACACCGCCAGTGGATGCCAGAATTTGCAGTGTGGTTTTCGA
  GCCTGCTGCCGCTCTGGCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207622 representing NM_001107222
  Red=Cloning site Green=Tags(s)
  
  MKQLVGRYCDKLSKTNFSNNNDFRALLQSLYATFKEFKMHEQIENEYIIGLLQQRSQTIYNVHSDNKLSE
  MLSLFEKGLKNVKNECEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEELKDIKKKVIAQ
  HCSQKDTAELLRGLSLWNQAEERQKVFKYSVDEKSDAEAEVSEHSTGITHLPPEVMLSIFSYLNPQELCR
  CSQVSTKWSQLAKTGSLWKHLYPVHWARGDWYSGPATELDTEPDEEWVRSRKDESRAFQEWDEDADIDES
  EESAEESVAISIAQMEKRLLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMSLRHLDLSGCEKITDMALEK
  ISRALGVLTSHQSGVLKSAGKTTSTPWTNKDITMPSTTQYACLHNLTNKGVGEEIDSEHTWTEPVSSESF
  TPPYVWMLDAEDLADIEDAVEWRHRNVESLCVMETASNFGCSSSGCYSKDIVGLRTSVCWQQHCASPAFA
  YCGHSFCCTGTALRTMSALPAASAVCRKALRTTLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDH
  GLRVLTLGGGLPYLEHLNLSGCLTVTGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFR
  ACCRSGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107222
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107222.1, NP_001100692.1
• RefSeq Size: 2658 bp
• RefSeq ORF: 1914 bp
• Locus ID: 305424
• Cytogenetics: 14q21
• MW: 71.7 kDa