Cacna1h (NM_153814) Rat Tagged ORF Clone

CAT#: RR207325

Cacna1h (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1H subunit (Cacna1h), (10 ug)

Product Images

Circular map for RR207325

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207325 representing NM_153814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGGGCACGCTGGCAGCGGACGAAGTCCGGGTGCCCCTGGGCGCTTCGCCGCCGGCCCCTGCAG
  CGCCGGTGAGAGCTTCCCCAGCGAGCCCTGGGGCGCCGGGGCGCGAGGAGCAGGGAGGATCCGGGTCGGG
  CGTGTTGGCTCCCGAGAGCCCAGGGACCGAGTGTGGTGCGGACCTGGGCGCCGACGAGGAACAGCCGGTC
  CCATACCCAGCTCTGGCTGCCACAGTCTTCTTCTGCCTCGGGCAAACCACGCGGCCGCGCAGCTGGTGCC
  TCCGACTGGTTTGTAACCCGTGGTTCGAGCACATCAGCATGCTGGTCATCATGCTGAACTGCGTGACACT
  GGGCATGTTCAGGCCCTGTGAGGATGTTGAGTGCCGCTCCGAACGTTGCAGCATCTTGGAGGCCTTCGAC
  GACTTCATCTTTGCCTTCTTCGCCGTGGAGATGGTGATCAAGATGGTGGCTTTGGGGCTGTTTGGGCAAA
  AATGCTACCTGGGTGACACCTGGAACAGGCTGGACTTCTTCATTGTCATGGCGGGCATGATGGAGTACTC
  TCTGGACGGACACAACGTGAGCCTCTCTGCCATCCGAACCGTGCGTGTGCTGCGGCCCCTCCGCGCCATC
  AACCGAGTCCCCAGTATGCGGATCCTGGTCACTCTGCTGCTGGACACGCTGCCCATGCTTGGGAATGTCC
  TCCTCCTCTGCTTCTTCGTCTTCTTCATCTTCGGCATTGTTGGGGTCCAGCTCTGGGCTGGCCTGCTTCG
  GAACCGATGCTTCCTGGACAGCGCCTTCGTCAGGAACAACAACCTGACCTTCTTGCGGCCATACTACCAG
  ACGGAGGAGGGTGAGGAGAACCCTTTCATCTGCTCCTCCCGCCGTGACAACGGCATGCAGAAGTGCTCGC
  ACATCCCCAGCCGCCGTGAGCTTCGAGTGCAGTGCACACTCGGCTGGGAGGCCTATGGGCAGCCACAGGC
  TGAGGATGGGGGTGCTGGCCGCAACGCCTGTATCAACTGGAACCAGTATTACAACGTGTGCCGCTCGGGG
  GAATTCAACCCTCACAACGGTGCCATCAACTTCGACAACATTGGCTACGCTTGGATTGCCATCTTCCAGG
  TCATCACACTGGAGGGCTGGGTGGACATCATGTACTACGTCATGGATGCCCACTCGTTCTACAACTTCAT
  CTACTTCATCCTCCTCATCATTATGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATAGCCACA
  CAGTTCTCAGAGACAAAGCAAAGGGAAAACCAGCTGATGCGAGAACAGCGGGCCCGCTATCTGTCCAACG
  ACAGCACTCTGGCCAGCTTCTCAGAGCCCGGCAGCTGCTACGAGGAGCTCCTCAAGTATGTAGGCCACAT
  CTTCCGGAAGGTTAAACGCCGTAGCCTGCGTCTTTATGCCCGCTGGCAGAGCCGCTGGCGTAAGAAGGTG
  GATCCCAGCAGTACCGTGCATGGCCAAGGCCCTGGGCGGCGGCCACGACGGGCAGGCAGGCGTACAGCTT
  CAGTGCACCATCTGGTCTACCACCACCACCACCACCATCACCACCATTACCACTTTAGCCACGGTGGCCC
  ACGCAGGCCCAGCCCAGAGCCAGGTGCTGGTGACAACAGGTTGGTCAGGGCCTGTGCGCCACCCTCGCCG
  CCATCCCCAGGCCATGGGCCACCAGACTCTGAGTCTGTGCACAGTATCTACCATGCTGACTGCCACGTGG
  AGGGGCCGCAGGAACGAGCCCGAGTGGCACACTCCATAGCCACTGCTGCTAGCCTCAAGCTGGCCTCAGG
  TTTGGGTACCATGAACTACCCCACCATCCTACCTTCAGGAACAGTCAACAGCAAAGGTGGCACCAGCTCA
  CGACCCAAGGGGCTACGAGGTGCTGGCGCCCCAGGGGCTGCAGTACACAGCCCTCTGAGCCTGGGAAGCC
  CCAGACCCTATGAGAAGATCCAGGATGTGGTGGGAGAACAAGGACTAGGCCGAGCCTCTAGCCACCTGTC
  AGGCCTGAGTGTGCCTTGCCCCCTGCCCAGCCCCCAGGCTGGCACGCTGACCTGTGAGCTGAAGAGCTGC
  CCATATTGTGCCAGCGCCCTGGAGGACCCCGAGTTTGAATTCAGTGGCTCAGAGAGCGGGGACTCGGATG
  CCCACGGAGTCTATGAGTTTACCCAGGATGTACGGCATGGGGATTGTCGGGACCCTGTGCAGCAGCCCCA
  TGAAGTGGGCACACCAGGCCACAGCAATGAGCGGCGGCGGACACCACTGCGGAAGGCCTCACAACCAGGA
  GGGATAGGCCACCTCTGGGCATCCTTCAGTGGCAAGCTACGTCGCATTGTAGACAGCAAGTACTTCAACC
  GAGGCATCATGGCAGCCATCCTCGTCAATACTCTGAGCATGGGCGTTGAGTATCATGAACAGCCTGAGGA
  GCTGACCAACGCCCTGGAGATAAGCAACATCGTGTTCACCAGCATGTTTGCCTTGGAGATGCTACTGAAG
  CTGCTGGCCTGCGGCCCACTGGGATACATCCGGAACCCCTACAACATCTTCGATGGCATTGTTGTCGTCA
  TAAGTGTCTGGGAGATCGTGGGGCAGGCAAACGGTGGCCTGTCTGTGCTGCGCACCTTCAGGCTGCTGCG
  GGTGCTGAAGCTGGTGCGCTTCCTGCCGGCCCTGCGGCGCCAGCTCGTGGTGCTCATGAGGACCATGGAC
  AACGTGGCCACCTTCTGCATGCTCCTCATGCTGTTCATCTTCATCTTCAGCATCCTGGGCATGCACCTGT
  TCGGCTGTAAGTTCAGCCTGAAGACAGACTCTGGAGACACCGTCCCTGACAGGAAGAACTTCGACTCCCT
  ACTGTGGGCCATCGTCACCGTGTTTCAGATCTTGACACAGGAAGACTGGAACGTGGTTCTGTACAACGGC
  ATGGCCTCCACTTCGTCCTGGGCCGCCCTTTACTTTGTGGCCCTCATGACCTTTGGGAACTATGTGCTCT
  TCAACCTGCTGGTAGCCATCCTGGTGGAAGGTTTCCAGGCAGAGGGTGACGCCACCAGATCTGACACCGA
  CGAGGATAAGACGTCTACCCAGCTAGAGGGAGATTTCGATAAGCTCAGAGATCTTCGAGCCACAGAGATG
  AAGATGTATTCACTGGCAGTGACCCCTAACGGGCACCTAGAGGGCCGAGGCAGCCTGCCGCCGCCCCTCA
  TCACTCACACGGCAGCTACGCCTATGCCTACTCCCAAAAGCTCCCCAAACCTGGACGTGGCCCATGCTCT
  CCTGGACTCTCGGCGCAGCAGCAGCGGCTCTGTGGACCCCCAGCTGGGGGACCAGAAGTCTCTGGCCAGC
  CTCCGCAGCTCCCCTTGCACCCCATGGGGCCCCAACAGCGCTGGGAGCAGCAGGCGCTCCAGTTGGAACA
  GCCTGGGCCGCGCACCCAGCCTCAAACGCCGCAACCAGTGTGGGGAGCGCGAGTCCCTGCTCTCTGGAGA
  GGGGAAGGGCAGCACCGATGACGAGGCCGAGGACAGCAGACCAAGCACGGGAACCCACCCAGGGGCCTCG
  CCAGGGCCCCGAGCCACGCCACTGCGGCGTGCCGAGTCATTGGACCACCGCAGCACGCTGGACCTGTGTC
  CACCACGGCCTGCGGCCCTCCTGCCGACCAAGTTCCATGACTGCAACGGGCAGATGGTGGCCCTGCCCAG
  CGAGTTCTTTCTGCGCATCGACAGCCACAAGGAGGATGCAGCGGAGTTTGATGATGACATAGAGGATAGC
  TGCTGCTTCCGTCTACACAAAGTGCTGGAACCCTATGCACCCCAGTGGTGCCGTAGCCGGGAGTCCTGGG
  CCCTGTATCTCTTCCCACCGCAGAACAGGCTACGCGTCTCCTGCCAGAAAGTCATCGCACACAAGATGTT
  TGACCACGTGGTCCTTGTCTTCATCTTCCTCAACTGTATCACCATTGCTCTGGAGAGGCCAGACATTGAC
  CCAGGCAGCACTGAGCGGGCCTTCCTCAGCGTCTCCAACTACATCTTCACAGCCATCTTCGTGGTGGAGA
  TGATGGTGAAGGTGGTAGCCCTGGGACTGCTGTGGGGTGAACATGCCTACCTACAGAGCAGTTGGAATGT
  GCTGGACGGGCTGCTTGTCCTGGTATCCCTGGTTGACATCATCGTGGCCATGGCCTCAGCTGGCGGTGCC
  AAGATCCTAGGCGTCCTGCGTGTGCTGCGCCTGCTGCGGACCCTGAGGCCTCTGAGGGTCATCAGCCGAG
  CTCCAGGCCTCAAGCTGGTTGTAGAGACTCTGATATCATCGCTCAGGCCCATTGGGAACATCGTCCTCAT
  CTGCTGCGCCTTCTTCATCATCTTTGGCATCCTCGGGGTGCAGCTTTTCAAGGGCAAATTCTACTACTGC
  GAGGGCACAGATACCAGGAATATCACCACCAAGGCCGAGTGCCATGCTGCCCACTACCGCTGGGTGAGGC
  GCAAATACAACTTTGACAACCTGGGTCAGGCGCTGATGTCTCTGTTCGTGCTGTCATCTAAGGATGGCTG
  GGTAAACATCATGTATGACGGGCTGGATGCCGTGGGCATCGACCAGCAGCCCGTGCAGAACCACAACCCC
  TGGATGCTGCTCTACTTCATCTCCTTCCTGCTCATCGTCAGCTTCTTCGTGCTCAACATGTTTGTGGGCG
  TGGTGGTGGAGAACTTCCACAAGTGCCGGCAGCACCAGGAGGCTGAGGAGGCTCGGCGCCGGGAGGAGAA
  ACGGCTGCGGCGCCTGGAGAGGAGGCGCAGGAAGGCCCAGCGCCGGCCCTACTACGCAGACTATTCACAC
  ACTCGCCGCTCCATCCATTCGCTGTGCACCAGCCACTACCTGGACCTCTTCATCACCTTCATCATCTGCC
  TCAATGTCATCACCATGTCCATGGAGCACTACAACCAGCCCAAGTCCCTGGATGAGGCCCTCAAGTACTG
  CAACTACGTCTTTACCATCGTCTTCGTCTTTGAGGCTGCACTGAAGCTGGTGGCCTTTGGGTTCCGGAGG
  TTCTTCAAGGACAGGTGGAACCAGCTGGACTTGGCCATCGTCCTCCTATCCATCATGGGCATTGCGCTGG
  AGGAGATTGAGATGAACGCCGCCCTGCCCATCAATCCCACCATCATCCGCATCATGCGTGTGCTTCGAAT
  CGCCCGTGTGCTGAAGCTACTGAAGATGGCCACAGGCATGCGCGCCTTGCTGGATACTGTGGTTCAAGCT
  CTGCCTCAGGTAGGGAACCTTGGTCTTCTTTTCATGCTCCTGTTTTTTATCTATGCTGCCCTGGGAGTGG
  AGCTGTTTGGGAGGCTAGAGTGCAGCGAGGATAACCCCTGCGAGGGCCTGAGCAGGCACGCTACCTTCAC
  CAACTTCGGCATGGCCTTCCTCACACTGTTCCGAGTGTCCACTGGGGACAACTGGAATGGGATTATGAAG
  GATACCCTCCGTGAGTGTACCCGTGAGGACAAGCACTGCCTCAGCTACCTGCCCGCGCTCTCACCCGTCT
  ACTTCGTCACCTTCATGCTGGTGGCTCAGTTCGTGCTGGTCAATGTGGTGGTGGCCGTGCTCATGAAGCA
  CCTGGAGGAGAGCAACAAGGAGGCCCGCGAGGATGCAGAGATGGACGCCGAGATCGAGCTGGAGATGGCA
  CAGGGGTCCACAGCCCAGCCCCCACCTACAGCACAGGAAAGCCAAGGTACCCAGCCAGACACCCCGAACC
  TCCTGGTCGTGCGAAAAGTATCTGTGTCCAGGATGCTCTCGCTGCCCAATGACAGCTACATGTTCAGGCC
  GGTGGCTCCCGCGGCTGCCCCACATTCCCACCCACTGCAGGAAGTGGAGATGGAGACCTACACAGGCCCG
  GTCACCTCTGCTCACTCGCCACCCCTGGAGCCCCGCGCCTCTTTCCAGGTCCCATCAGCCGCGTCCTCCC
  CAGCCAGGGTCAGTGACCCCCTTTGTGCCCTTTCACCCCGGGGTACACCCCGCTCTCTGAGTCTCTCACG
  GATACTCTGCAGACAGGAGGCCATGCACTCTGAGTCCCTGGAAGGGAAGGTTGATGATGTTGGAGGAGAC
  AGCATCCCAGACTACACAGAGCCTGCTGAAAATATGTCCACGAGCCAGGCATCAACAGGTGCCCCGAGGT
  CCCCTCCGTGCTCCCCGCGACCTGCCAGCGTCCGTACCCGCAAGCACACGTTTGGGCAACGCTGCATCTC
  CAGCCGCCCTCCCACCCTGGGAGGAGATGAGGCTGAAGCAGCAGACCCAGCAGATGAGGAGGTCAGCCAC
  ATCACCAGCTCAGCCCACCCCTGGCCGGCTACAGAGCCCCACAGCCCTGAGGCCTCCCCAACAGCCTCTC
  CTGTGAAAGGCACAATGGGCAGTGGGCGGGACCCACGCAGGTTCTGCAGTGTAGATGCTCAGAGCTTCCT
  GGACAAACCAGGTCGGCCAGATGCACAACGGTGGTCCTCAGTGGAACTGGATAACGGAGAAAGCCACCTA
  GAGTCCGGGGAAGTGAGGGGCCGGGCCTCAGAGCTCGAACCAGCTCTTGGGTCACGAAGGAAGAAGAAGA
  TGAGCCCTCCCTGCATCTCCATTGAACCTCCCACTAAGGATGAGGGCTCTTCCCGGCCCCCTGCAGCCGA
  AGGAGGCAACACTACCCTGAGGCGCCGAACCCCATCCTGTGAGGCTGCCCTCCATAGGGACTGCCCAGAG
  CCTACAGAAGGCCCAGGCACCGGAGGGGACCCTGTAGCCAAGGGTGAGCGCTGGGGCCAGGCCTCTTGCC
  GAGCAGAGCATCTGACTGTCCCCAACTTTGCCTTTGAGCCTCTGGACATGGGCGGACCTGGTGGAGACTG
  TTTCTTGGACAGTGACCAAAGTGTGACCCCAGAACCCAGAGTTTCCTCTTTGGGGGCTATAGTGCCTCTG
  ATACTAGAAACTGAACTTTCTATGCCCTCTGGTGACTGCCCAGAGAAGGAACAAGGACTGTACCTCACTG
  TGCCCCAGACCCCCTTGAAGAAACCAGGGTCTACCCCAGCCACTCCTGCCCCAGATGACAGTGGAGATGA
  GCCTGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207325 representing NM_153814
  Red=Cloning site Green=Tags(s)
  
  MTEGTLAADEVRVPLGASPPAPAAPVRASPASPGAPGREEQGGSGSGVLAPESPGTECGADLGADEEQPV
  PYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFD
  DFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNVSLSAIRTVRVLRPLRAI
  NRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQ
  TEEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGGAGRNACINWNQYYNVCRSG
  EFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIMGSFFMINLCLVVIAT
  QFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKV
  DPSSTVHGQGPGRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGPRRPSPEPGAGDNRLVRACAPPSP
  PSPGHGPPDSESVHSIYHADCHVEGPQERARVAHSIATAASLKLASGLGTMNYPTILPSGTVNSKGGTSS
  RPKGLRGAGAPGAAVHSPLSLGSPRPYEKIQDVVGEQGLGRASSHLSGLSVPCPLPSPQAGTLTCELKSC
  PYCASALEDPEFEFSGSESGDSDAHGVYEFTQDVRHGDCRDPVQQPHEVGTPGHSNERRRTPLRKASQPG
  GIGHLWASFSGKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLK
  LLACGPLGYIRNPYNIFDGIVVVISVWEIVGQANGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMD
  NVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNG
  MASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTQLEGDFDKLRDLRATEM
  KMYSLAVTPNGHLEGRGSLPPPLITHTAATPMPTPKSSPNLDVAHALLDSRRSSSGSVDPQLGDQKSLAS
  LRSSPCTPWGPNSAGSSRRSSWNSLGRAPSLKRRNQCGERESLLSGEGKGSTDDEAEDSRPSTGTHPGAS
  PGPRATPLRRAESLDHRSTLDLCPPRPAALLPTKFHDCNGQMVALPSEFFLRIDSHKEDAAEFDDDIEDS
  CCFRLHKVLEPYAPQWCRSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDID
  PGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAMASAGGA
  KILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYC
  EGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNP
  WMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSH
  TRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRR
  FFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQA
  LPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMK
  DTLRECTREDKHCLSYLPALSPVYFVTFMLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEMA
  QGSTAQPPPTAQESQGTQPDTPNLLVVRKVSVSRMLSLPNDSYMFRPVAPAAAPHSHPLQEVEMETYTGP
  VTSAHSPPLEPRASFQVPSAASSPARVSDPLCALSPRGTPRSLSLSRILCRQEAMHSESLEGKVDDVGGD
  SIPDYTEPAENMSTSQASTGAPRSPPCSPRPASVRTRKHTFGQRCISSRPPTLGGDEAEAADPADEEVSH
  ITSSAHPWPATEPHSPEASPTASPVKGTMGSGRDPRRFCSVDAQSFLDKPGRPDAQRWSSVELDNGESHL
  ESGEVRGRASELEPALGSRRKKKMSPPCISIEPPTKDEGSSRPPAAEGGNTTLRRRTPSCEAALHRDCPE
  PTEGPGTGGDPVAKGERWGQASCRAEHLTVPNFAFEPLDMGGPGGDCFLDSDQSVTPEPRVSSLGAIVPL
  ILETELSMPSGDCPEKEQGLYLTVPQTPLKKPGSTPATPAPDDSGDEPV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153814
• ORF Size: 7077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153814.2, NP_722521.1
• RefSeq Size: 7871 bp
• RefSeq ORF: 7080 bp
• Locus ID: 114862
• UniProt ID: Q9EQ60
• Cytogenetics: 10q12
• MW: 261.1 kDa
• Gene Summary: acts as a low voltage gated calcium channel; conducts calcium current; involved in neuronal physiology [RGD, Feb 2006]