Myc (NM_012603) Rat Tagged ORF Clone

CAT#: RR207206

Myc (Myc-DDK-tagged ORF) - Rat myelocytomatosis oncogene (Myc), (10 ug)

Product Images

Circular map for RR207206

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myc
• Synonyms: c-myc; mMyc; RNCMYC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207206 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGAATTTCCTTTGGGAGGTGGAAAACCCGACAGTCACGACGATGCCCCTCAACGTGAGCTTCGCTAACA
  GGAACTATGACCTCGACTACGACTCGGTGCAGCCCTATTTCATCTGCGACGAGGAAGAGAATTTCTATCA
  CCAGCAACAGCAGAGCGAGCTGCAGCCGCCCGCACCCAGTGAGGATATCTGGAAGAAATTCGAGCTGCTG
  CCCACCCCGCCCCTGTCCCCCAGCCGCCGCTCCGGGCTCTGCTCTCCGTCCTATGTTGCGGTCGCTACGT
  CCTTCTCCCCAAGGGAGGACGATGACGGTGGCGGTGGCAACTTCTCCACCGCCGATCAGCTGGAGATGAT
  GACCGAGCTACTTGGAGGAGACATGGTGAATCAGAGCTTCATCTGCGATCCTGACGATGAGACCTTCATC
  AAGAACATCATCATCCAGGACTGTATGTGGAGCGGCTTCTCGGCCGCTGCCAAACTGGTCTCCGAGAAGC
  TGGCCTCTTACCAGGCTGCGCGCAAAGACAGCACCAGCCTGAGCCCCGCCCGCGGGCACAGCGTCTGCTC
  CACCTCCAGCCTGTACCTGCAGGACCTCACCGCCGCAGCGTCCGAGTGCATCGACCCCTCAGTGGTCTTC
  CCCTACCCGCTCAACGACAGCAGCTCGCCCAAATCCTGTACCTCGTCCGATTCCACGGCCTTCTCTTCTT
  CCTCGGACTCGCTGCTGTCCTCCGAGTCCTCCCCACGGGCCACCCCTGAGCCCCTAGTGCTGCATGAAGA
  GACACCGCCCACCACCAGCAGCGACTCTGAAGAAGAACAAGATGATGAGGAAGAAATTGATGTGGTGTCT
  GTGGAAAAGAGGCAACCCCCTGCCAAGAGGTCCGAGTCAGGGTCATCCCCATCAAGAGGCCACAGCAAAC
  CTCCACACAGCCCACTGGTCCTCAAGAGGTGCCATGTCTCTACTCACCAGCACAATTATGCAGCACCCCC
  CTCCACAAGGAAGGACTATCCAGCTGCCAAGAGGGCCAAGTTGGACAGTGGCAGGGTCCTGAAACAGATC
  AGCAACAACCGCAAATGCTCCAGCCCCAGGTCCTCAGACACCGAGGAAAACGACAAGAGGCGGACACACA
  ACGTCTTGGAACGTCAGAGGAGAAACGAGCTGAAGCGTAGCTTTTTTGCCCTGCGCGACCAGATCCCTGA
  GTTGGAAAACAACGAAAAGGCCCCCAAGGTAGTTATCCTCAAAAAAGCCACCGCCTACATCCTGTCCGTT
  CAAGCAGATGAGCACAAACTCATCTCAGAAAAGGACTTACTGAGGAAACGGCGAGAACAGTTGAAACACA
  AACTCGAACAGCTTCGAAACTCTGGTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207206 protein sequence
  Red=Cloning site Green=Tags(s)
  
  LNFLWEVENPTVTTMPLNVSFANRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELL
  PTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFI
  KNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVF
  PYPLNDSSSPKSCTSSDSTAFSSSSDSLLSSESSPRATPEPLVLHEETPPTTSSDSEEEQDDEEEIDVVS
  VEKRQPPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQI
  SNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSV
  QADEHKLISEKDLLRKRREQLKHKLEQLRNSGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012603
• ORF Size: 1359 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012603.2, NP_036735.2
• RefSeq Size: 2355 bp
• RefSeq ORF: 1362 bp
• Locus ID: 24577
• UniProt ID: P09416
• Cytogenetics: 7q33
• MW: 50.5 kDa
• Gene Summary: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Rat mRNA also has a similarly placed CUG upstream of the AUG start site, suggesting that it may also produce two Myc proteins. [provided by RefSeq, Jul 2008]