Htr2c (NM_012765) Rat Tagged ORF Clone

CAT#: RR207006

Htr2c (Myc-DDK-tagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c), (10 ug)

Product Images

Circular map for RR207006

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Htr2c
• Synonyms: 5-HT2C; 5-HTR2C; 5HT-1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207006 representing NM_012765
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACCTTGGCAACGCGGTGCGCTCGCTCCTGATGCACCTAATCGGCCTATTGGTTTGGCAATTCG
  ATATTTCCATAAGTCCAGTAGCAGCTATAGTAACTGACACTTTTAATTCCTCCGATGGTGGACGCTTGTT
  TCAATTCCCGGACGGGGTACAAAACTGGCCAGCACTTTCAATCGTCGTGATTATAATCATGACAATAGGG
  GGCAACATTCTTGTTATCATGGCAGTAAGCATGGAGAAGAAACTGCACAATGCAACCAATTACTTCTTAA
  TGTCCCTAGCCATTGCTGATATGCTGGTGGGACTACTTGTCATGCCCCTGTCCCTGCTTGCTATTCTTTA
  TGATTATGTCTGGCCTTTACCTAGATATTTGTGCCCCGTCTGGATTTCACTAGATGTGCTATTTTCAACT
  GCGTCCATCATGCACCTCTGCGCCATATCGCTGGACCGGTATGTAGCAATACGTAATCCTATTGAGCATA
  GCCGGTTCAATTCGCGGACTAAGGCCATCATGAAGATTGCCATCGTTTGGGCAATATCAATAGGAGTTTC
  AGTTCCTATCCCTGTGATTGGACTGAGGGACGAAAGCAAAGTGTTCGTGAATAACACCACGTGCGTGCTC
  AATGACCCCAACTTCGTTCTCATCGGGTCCTTCGTGGCATTCTTCATCCCGTTGACGATTATGGTGATCA
  CCTACTTCTTAACGATCTACGTCCTGCGCCGTCAAACTCTGATGTTACTTCGAGGTCACACCGAGGAGGA
  ACTGGCTAATATGAGCCTGAACTTTCTGAACTGCTGCTGCAAGAAGAATGGTGGTGAGGAAGAGAACGCT
  CCGAACCCTAATCCAGATCAGAAACCACGTCGAAAGAAGAAAGAAAAGCGTCCCAGAGGCACCATGCAAG
  CTATCAACAACGAAAAGAAAGCTTCCAAAGTCCTTGGCATTGTATTCTTTGTGTTTCTGATCATGTGGTG
  CCCGTTTTTCATCACCAATATCCTGTCGGTTCTTTGTGGGAAGGCCTGTAACCAAAAGCTAATGGAGAAG
  CTTCTCAATGTGTTTGTGTGGATTGGCTATGTGTGTTCAGGCATCAATCCTCTGGTGTACACTCTCTTTA
  ATAAAATTTACCGAAGGGCTTTCTCTAAATATTTGCGCTGCGATTATAAGCCAGACAAAAAGCCTCCTGT
  TCGACAGATTCCTAGGGTTGCTGCCACTGCTTTGTCTGGGAGGGAGCTCAATGTTAACATTTATCGGCAT
  ACCAATGAACGTGTGGCTAGGAAAGCTAATGACCCTGAGCCTGGCATAGAGATGCAGGTGGAGAACTTAG
  AGCTGCCAGTCAACCCCTCTAATGTGGTCAGCGAGAGGATTAGTAGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207006 representing NM_012765
  Red=Cloning site Green=Tags(s)
  
  MVNLGNAVRSLLMHLIGLLVWQFDISISPVAAIVTDTFNSSDGGRLFQFPDGVQNWPALSIVVIIIMTIG
  GNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFST
  ASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVL
  NDPNFVLIGSFVAFFIPLTIMVITYFLTIYVLRRQTLMLLRGHTEEELANMSLNFLNCCCKKNGGEEENA
  PNPNPDQKPRRKKKEKRPRGTMQAINNEKKASKVLGIVFFVFLIMWCPFFITNILSVLCGKACNQKLMEK
  LLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSKYLRCDYKPDKKPPVRQIPRVAATALSGRELNVNIYRH
  TNERVARKANDPEPGIEMQVENLELPVNPSNVVSERISSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012765
• ORF Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012765.3, NP_036897.2
• RefSeq Size: 4402 bp
• RefSeq ORF: 1383 bp
• Locus ID: 25187
• UniProt ID: P08909
• Cytogenetics: Xq34
• MW: 52.4 kDa
• Gene Summary: Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008]