Syncrip (NM_001047916) Rat Tagged ORF Clone

CAT#: RR206980

Syncrip (Myc-DDK-tagged ORF) - Rat synaptotagmin binding, cytoplasmic RNA interacting protein (Syncrip), (10 ug)

Product Images

Circular map for RR206980

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syncrip
• Synonyms: Ab2-339; hnRNP Q
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206980 representing NM_001047916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAACATGTTAATGGAAATGGTACTGAAGAGCCCATGGATACTACTTCAGCAGTTATCCATT
  CAGAAAATTTTCAGACATTGCTTGATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTA
  CGTTGCAGGTCAGAGGAAGTATGGGGGGCCACCTCCAGATTCTGTTTATTCAGGTCAGCAGCCTTCTGTT
  GGCACTGAGATATTTGTGGGGAAGATCCCCAGAGATCTGTTTGAAGATGAGCTTGTTCCATTGTTTGAGA
  AAGCTGGACCTATATGGGATCTTCGTTTAATGATGGATCCACTTACTGGTCTCAACAGAGGTTATGCCTT
  TGTCACTTTTTGTACAAAAGAAGCAGCACAAGAGGCTGTTAAACTGTATAATAATCATGAAATTCGTTCC
  GGGAAACATATTGGTGTCTGCATCTCAGTTGCCAACAATAGGCTTTTTGTGGGCTCTATTCCTAAGAGTA
  AAACCAAGGAACAGATTCTTGAAGAATTTAGTAAAGTGACAGAGGGTCTCACAGATGTCATTTTATACCA
  CCAACCTGATGACAAGAAAAAAAACAGAGGCTTTTGCTTTCTTGAATATGAAGATCATAAAACAGCTGCC
  CAGGCAAGGCGTAGGCTAATGAGTGGTAAAGTTAAAGTCTGGGGAAATGTTGGAACTGTTGAATGGGCTG
  ATCCTATTGAAGATCCTGATCCTGAAGTTATGGCAAAGGTAAAAGTGCTGTTTGTACGCAACCTTGCCAA
  CACTGTAACAGAAGAAATTTTAGAAAAGTCGTTTAGTCAGTTTGGGAAACTGGAACGAGTGAAGAAGCTA
  AAAGATTATGCTTTCATTCATTTTGATGAGAGAGATGGTGCTGTCAAGGCTATGGAAGAAATGAATGGTA
  AAGACTTGGAGGGAGAAAACATTGAAATTGTTTTTGCTAAGCCACCAGATCAGAAGAGGAAAGAAAGAAA
  AGCTCAGAGGCAAGCAGCAAAGAATCAAATGTATGATGATTACTACTATTATGGTCCACCTCATATGCCC
  CCTCCAACAAGAGGTCGAGGGCGTGGAGGTAGAGGTGGCTATGGATATCCTCCAGATTATTATGGATACG
  AAGATTATTATGATTATTATGGTTATGATTACCATAACTATCGTGGTGGATATGAAGATCCATATTATGG
  TTATGAAGATTTTCAAGTTGGAGCTAGAGGAAGGGGTGGTAGAGGAGCAAGGGGTGCTGCTCCATCCAGA
  GGTCGCGGGGCTGCTCCTCCCCGTGGTAGAGCCGGTTATTCACAGAGAGGAGGCCCTGGATCAGCAAGAG
  GCGTTCGTGGTGCGAGAGGAGGTGCCCAACAACAAAGAGGCCGCGGGGTACGTGGTGCAAGGGGTGGCCG
  CGGTGGAAATGTAGGAGGAAAGCGCAAAGCTGATGGGTACAACCAGCCAGATTCCAAGCGGCGCCAGACC
  AATAATCAGAACTGGGGCTCCCAACCCATTGCTCAGCAACCGCTCCAAGGTGGTGATCATTCTGGTAACT
  ATGGTTACAAATCTGAAAACGAGGAGTTTTATCAGGATACTTTTGGGCAACAGTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206980 representing NM_001047916
  Red=Cloning site Green=Tags(s)
  
  MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGQRKYGGPPPDSVYSGQQPSV
  GTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTFCTKEAAQEAVKLYNNHEIRS
  GKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGFCFLEYEDHKTAA
  QARRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVTEEILEKSFSQFGKLERVKKL
  KDYAFIHFDERDGAVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMP
  PPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSR
  GRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGVRGARGGRGGNVGGKRKADGYNQPDSKRRQT
  NNQNWGSQPIAQQPLQGGDHSGNYGYKSENEEFYQDTFGQQWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047916
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047916.2, NP_001041381.1
• RefSeq Size: 1648 bp
• RefSeq ORF: 1602 bp
• Locus ID: 363113
• UniProt ID: Q7TP47
• Cytogenetics: 8q31
• MW: 59.7 kDa
• Gene Summary: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Is associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Binds to apoB mRNA AU-rich sequences. Part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. May be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins (By similarity).[UniProtKB/Swiss-Prot Function]