Hnf4a (NM_022180) Rat Tagged ORF Clone

CAT#: RR206976

Hnf4a (Myc-DDK-tagged ORF) - Rat hepatocyte nuclear factor 4, alpha (Hnf4a), (10 ug)

Product Images

Circular map for RR206976

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnf4a
• Synonyms: Hnf4; Hnf4alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206976 representing NM_022180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACTCTCTAAAACCCTCGCCGACATGGACATGGCTGACTACAGTGCTGCCTTGGACCCAGCCTACA
  CCACCCTGGAGTTTGAAAATGTGCAGGTGTTGACCATGGGCAATGACACATCCCCATCTGAAGGTGCCAA
  CCTCAACTCATCCAACAGCCTGGGTGTCAGTGCCCTGTGTGCCATCTGTGGCGATCGGGCCACTGGCAAA
  CACTACGGAGCCTCAAGCTGTGACGGCTGCAAGGGATTCTTCAGGAGGAGCGTGAGGAAGAACCACATGT
  ACTCCTGCAGGTTTAGCAGGCAGTGCGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCT
  CAAGAAGTGCTTCCGGGCTGGCATGAAGAAAGAAGCCGTCCAAAATGAGCGGGATCGGATCAGCACGCGG
  AGGTCAAGCTACGAGGACAGCAGCCTACCCTCCATTAATGCGCTCCTGCAGGCAGAGGTCCTGTCTCAGC
  AGATCACCTCCCCCATCTCTGGGATCAATGGCGACATTCGGGCCAAGAGGATTGCCAACATCACGGATGT
  GTGTGAGTCTATGAAGGAGCAGCTGCTGGTTCTGGTCGAATGGGCCAAGTACATCCCGGCCTTCTGTGAA
  CTTCTTCTGGATGACCAGGTGGCGCTGCTCAGAGCCCACGCTGGTGAGCACCTGCTGCTTGGAGCCACCA
  AGAGGTCCATGGTGTTCAAGGATGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGA
  GCTAGCAGAGATGAGCCGTGTGTCCATTCGCATCCTCGATGAGCTGGTCTTGCCCTTCCAAGAGCTGCAG
  ATCGATGATAATGAATACGCCTGCCTCAAAGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGTG
  ACCCAGGCAAGATCAAGCGGCTGCGGTCACAGGTGCAGGTGAGCCTGGAGGATTACATCAACGACCGGCA
  GTATGACTCTCGGGGTCGTTTTGGAGAGCTGCTGCTGCTCCTGCCCACTCTGCAGAGCATTACCTGGCAG
  ATGATCGAGCAGATCCAGTTCATCAAGCTCTTTGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGC
  TGCTTGGAGGGTCTGCCAGTGACGCGCCCCACGCCCACCACCCCCTGCACCCTCACCTGATGCAAGAACA
  CATGGGCACCAATGTCATAGTTGCCAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTGTGAGTGG
  CCCCGGCCCAGGGGGCAGGCAGCCACCCCTGAGACTCCACAGCCATCACCACCAAGTGGCTCTGGATCTG
  AATCCTACAAGCTCCTGCCAGGAGCCATCACCACCATCGTCAAGCCTCCCTCTGCCATCCCCCAGCCAAC
  GATCACCAAGCAGGAAGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206976 representing NM_022180
  Red=Cloning site Green=Tags(s)
  
  MRLSKTLADMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGK
  HYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTR
  RSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKRIANITDVCESMKEQLLVLVEWAKYIPAFCE
  LLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQ
  IDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ
  MIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEW
  PRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022180
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022180.2, NP_071516.2
• RefSeq Size: 1446 bp
• RefSeq ORF: 1425 bp
• Locus ID: 25735
• UniProt ID: P22449
• Cytogenetics: 3q42
• MW: 53.2 kDa
• Gene Summary: steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006]