Tnni3 (NM_017144) Rat Tagged ORF Clone

CAT#: RR206860L4

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  • LentiORF®

Lenti ORF clone of Tnni3 (mGFP-tagged ORF) - Rat troponin I type 3 (cardiac) (Tnni3), (10 ug)



  "NM_017144" in other vectors (3)

Special Offer: 20% off on control vector. Use code "Shuttle20".

USD 864.00


Availability*
8 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag mGFP
Symbol Tnni3
Synonyms cTNI; TnI
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RR206860).
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene     
ACCN NM_017144
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017144.2, NP_058840.1
RefSeq Size 829 bp
RefSeq ORF 636 bp
Locus ID 29248
UniProt ID P23693
Cytogenetics 1q12
Gene Summary cardiac isoform of troponin I, which is a subunit of the troponin complex; inhibits actomyosin ATPase activity in muscle conraction [RGD, Feb 2006]

Other Versions

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Forward primer to sequence targets cloned in expression vectors.
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Reverse primer to sequence targets cloned in expression vectors.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.