Pla2g6 (NM_001005560) Rat Tagged ORF Clone

CAT#: RR206825

Pla2g6 (Myc-DDK-tagged ORF) - Rat phospholipase A2, group VI (cytosolic, calcium-independent) (Pla2g6), (10 ug)

Product Images

Circular map for RR206825

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pla2g6
• Synonyms: iPla2; PNPLA9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206825 representing NM_001005560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTCTTTGGACGCCTCGTTAACACCCTCAGTAGTGTCACCAACTTGTTCTCAAACCCATTCCGGG
  TAAAGGAGGTGTCCTTGGCTGACTATGCCTCAAGTGAACGTGTTCGAGAGGAAGGGCAGCTGATCCTGTT
  GCAGAATGCTTCCAATCGTACCTGGGACTGTGTCCTGGTCAGCCCTAGGAACCCACAGAGTGGCTTCCGA
  CTCTTCCAACTGGAGTCAGAGGCAGACGCCCTGGTGAACTTCCAGCAGTATTCCTCCCAGCTGCCACCGT
  TCTATGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTGCAGCACCTGACGGACCTCATTCGGAACCA
  CCCCAGCTGGACAGTGACGCACCTGGCCGTGGAACTTGGCATTCGGGAGTGTTTCCATCACAGCCGCATC
  ATCAGCTGCGCCAACAGCACAGAGAATGAGGAGGGCTGTACCCCACTACATCTGGCCTGCCGCAAGGGTG
  ACAGTGAGATTCTGGTGGAGTTGGTACAGTACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA
  GACCGCCTTCCATTATGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGAAAGAATGCCTCA
  GCCGGCCTGAACCAGGTGAACAACCAAGGGCTGACGCCGCTGCACCTGGCCTGCCAGATGGGGAAGCAGG
  AGATGGTGCGTGTCCTGCTGCTCTGCAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA
  CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGTAACCAGATCCAC
  AGCAAGGATCCTCGCTATGGAGCCAGCCCGCTCCACTGGGCCAAGAACGCTGAGATGGCCCGAATGCTTC
  TGAAGAGGGGCTGTGACGTGGACAGCACAAGTGCCTCGGGGAACACAGCCCTGCACGTGGCGGTGACGCG
  CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTATGGGGCCAACGCAGGTGCCCGAGGAGAGCATGGG
  AACACACCACTGCATCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG
  CAGAAGTAGACACCCCGAATGACTTTGGGGAGACTCCTGCATTCATAGCTTCCAAGATCAGCAAGCTGAT
  TACCAGGAAGGCGCTCTTGACTCTGCTGAAAACGGTGGGGGCCGACTACCACTTCCCATTCATACAAGGG
  GTCTCCACGGAGCAGAGCTCTGCAGCTGGACCTCACCCCTTCTTCTCCCTGGACAGAACTCAGCCTCCAA
  CGATCAGCTTAAACAACCTAGAGCTTCAGGACCTCATGCCCGTCTCCCGAGCCCGGAAGCCAGCGTTCAT
  CCTGAGCTCCATGAGGGATGAGAAGCGAAGCCATGACCACCTGCTCTGCTTGGATGGAGGGGGCGTGAAG
  GGCCTGGTCATCATCCAGCTTCTCATTGCCATTGAGAAGGCCTCAGGTGTGGCTACCAAGGACCTCTTCG
  ACTGGGTGGCAGGAACCAGCACAGGCGGCATCCTGGCCCTGGCCATTCTACACAGTAAATCCATGGCCTA
  TATGCGTGGCGTGTACTTCCGTATGAAGGATGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCC
  CTGGAGGAGTTCCTGAAGCGTGAGTTTGGGGAGCACACCAAGATGACAGATGTCAAGAAACCCAAGGTGA
  TGCTGACAGGGACACTGTCAGACCGGCAGCCAGCAGAGCTCCACCTCTTCCGGAATTACGACGCCCCAGA
  GGCCGTTCGGGAACCTCGCTGTACCCCAAACATTAACCTGAAGCCGCCCACCCAGCCTGCAGACCAGTTA
  GTATGGCGGGCAGCCCGGAGCAGTGGGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCTTGGATG
  GTGGGCTGCTGGCCAACAACCCCACACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGATATGAT
  CCGCAAGGGCCAGGGCAACAAGGTGAAGAAACTCTCCATAGTCGTGTCTCTGGGGACAGGAAAGTCCCCT
  CAAGTGCCTGTAACCTGTGTAGATGTCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAGACTGTTTTTG
  GAGCCAAGGAACTGGGCAAGATGGTGGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGTGC
  CCGGGCCTGGTGTGAGATGGTCGGTATCCAGTACTTCAGGCTGAACCCCCAGCTAGGCTCAGACATCATG
  CTGGACGAGGTCAGTGATGCAGTGCTGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACC
  GGGAGGAGTTCCAGAAGCTTGTCCAGCTGCTGCTATCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206825 representing NM_001005560
  Red=Cloning site Green=Tags(s)
  
  MQFFGRLVNTLSSVTNLFSNPFRVKEVSLADYASSERVREEGQLILLQNASNRTWDCVLVSPRNPQSGFR
  LFQLESEADALVNFQQYSSQLPPFYESSVQVLHVEVLQHLTDLIRNHPSWTVTHLAVELGIRECFHHSRI
  ISCANSTENEEGCTPLHLACRKGDSEILVELVQYCHAQMDVTDNKGETAFHYAVQGDNPQVLQLLGKNAS
  AGLNQVNNQGLTPLHLACQMGKQEMVRVLLLCNARCNIMGPGGFPIHTAMKFSQKGCAEMIISMDSNQIH
  SKDPRYGASPLHWAKNAEMARMLLKRGCDVDSTSASGNTALHVAVTRNRFDCVMVLLTYGANAGARGEHG
  NTPLHLAMSKDNMEMVKALIVFGAEVDTPNDFGETPAFIASKISKLITRKALLTLLKTVGADYHFPFIQG
  VSTEQSSAAGPHPFFSLDRTQPPTISLNNLELQDLMPVSRARKPAFILSSMRDEKRSHDHLLCLDGGGVK
  GLVIIQLLIAIEKASGVATKDLFDWVAGTSTGGILALAILHSKSMAYMRGVYFRMKDEVFRGSRPYESGP
  LEEFLKREFGEHTKMTDVKKPKVMLTGTLSDRQPAELHLFRNYDAPEAVREPRCTPNINLKPPTQPADQL
  VWRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDMIRKGQGNKVKKLSIVVSLGTGKSP
  QVPVTCVDVFRPSNPWELAKTVFGAKELGKMVVDCCTDPDGRAVDRARAWCEMVGIQYFRLNPQLGSDIM
  LDEVSDAVLVNALWETEVYIYEHREEFQKLVQLLLSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005560
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005560.1, NP_001005560.1
• RefSeq Size: 3100 bp
• RefSeq ORF: 2424 bp
• Locus ID: 360426
• UniProt ID: P97570
• Cytogenetics: 7q34
• MW: 89.6 kDa
• Gene Summary: has Ca2+ independent phospholipase A2 activity; may play a role in regulating transmembrane ionic fluxes in pancreatic beta cells [RGD, Feb 2006]