Pla2g6 (NM_001005560) Rat Untagged Clone

CAT#: RN206825

Pla2g6 (untagged ORF) - Rat phospholipase A2, group VI (cytosolic, calcium-independent) (Pla2g6), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pla2g6
• Synonyms: iPla2; PNPLA9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206825 representing NM_001005560
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTCTTTGGACGCCTCGTTAACACCCTCAGTAGTGTCACCAACTTGTTCTCAAACCCATTCCGGG
  TAAAGGAGGTGTCCTTGGCTGACTATGCCTCAAGTGAACGTGTTCGAGAGGAAGGGCAGCTGATCCTGTT
  GCAGAATGCTTCCAATCGTACCTGGGACTGTGTCCTGGTCAGCCCTAGGAACCCACAGAGTGGCTTCCGA
  CTCTTCCAACTGGAGTCAGAGGCAGACGCCCTGGTGAACTTCCAGCAGTATTCCTCCCAGCTGCCACCGT
  TCTATGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTGCAGCACCTGACGGACCTCATTCGGAACCA
  CCCCAGCTGGACAGTGACGCACCTGGCCGTGGAACTTGGCATTCGGGAGTGTTTCCATCACAGCCGCATC
  ATCAGCTGCGCCAACAGCACAGAGAATGAGGAGGGCTGTACCCCACTACATCTGGCCTGCCGCAAGGGTG
  ACAGTGAGATTCTGGTGGAGTTGGTACAGTACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA
  GACCGCCTTCCATTATGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGAAAGAATGCCTCA
  GCCGGCCTGAACCAGGTGAACAACCAAGGGCTGACGCCGCTGCACCTGGCCTGCCAGATGGGGAAGCAGG
  AGATGGTGCGTGTCCTGCTGCTCTGCAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA
  CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGTAACCAGATCCAC
  AGCAAGGATCCTCGCTATGGAGCCAGCCCGCTCCACTGGGCCAAGAACGCTGAGATGGCCCGAATGCTTC
  TGAAGAGGGGCTGTGACGTGGACAGCACAAGTGCCTCGGGGAACACAGCCCTGCACGTGGCGGTGACGCG
  CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTATGGGGCCAACGCAGGTGCCCGAGGAGAGCATGGG
  AACACACCACTGCATCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG
  CAGAAGTAGACACCCCGAATGACTTTGGGGAGACTCCTGCATTCATAGCTTCCAAGATCAGCAAGCTGAT
  TACCAGGAAGGCGCTCTTGACTCTGCTGAAAACGGTGGGGGCCGACTACCACTTCCCATTCATACAAGGG
  GTCTCCACGGAGCAGAGCTCTGCAGCTGGACCTCACCCCTTCTTCTCCCTGGACAGAACTCAGCCTCCAA
  CGATCAGCTTAAACAACCTAGAGCTTCAGGACCTCATGCCCGTCTCCCGAGCCCGGAAGCCAGCGTTCAT
  CCTGAGCTCCATGAGGGATGAGAAGCGAAGCCATGACCACCTGCTCTGCTTGGATGGAGGGGGCGTGAAG
  GGCCTGGTCATCATCCAGCTTCTCATTGCCATTGAGAAGGCCTCAGGTGTGGCTACCAAGGACCTCTTCG
  ACTGGGTGGCAGGAACCAGCACAGGCGGCATCCTGGCCCTGGCCATTCTACACAGTAAATCCATGGCCTA
  TATGCGTGGCGTGTACTTCCGTATGAAGGATGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCC
  CTGGAGGAGTTCCTGAAGCGTGAGTTTGGGGAGCACACCAAGATGACAGATGTCAAGAAACCCAAGGTGA
  TGCTGACAGGGACACTGTCAGACCGGCAGCCAGCAGAGCTCCACCTCTTCCGGAATTACGACGCCCCAGA
  GGCCGTTCGGGAACCTCGCTGTACCCCAAACATTAACCTGAAGCCGCCCACCCAGCCTGCAGACCAGTTA
  GTATGGCGGGCAGCCCGGAGCAGTGGGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCTTGGATG
  GTGGGCTGCTGGCCAACAACCCCACACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGATATGAT
  CCGCAAGGGCCAGGGCAACAAGGTGAAGAAACTCTCCATAGTCGTGTCTCTGGGGACAGGAAAGTCCCCT
  CAAGTGCCTGTAACCTGTGTAGATGTCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAGACTGTTTTTG
  GAGCCAAGGAACTGGGCAAGATGGTGGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGTGC
  CCGGGCCTGGTGTGAGATGGTCGGTATCCAGTACTTCAGGCTGAACCCCCAGCTAGGCTCAGACATCATG
  CTGGACGAGGTCAGTGATGCAGTGCTGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACC
  GGGAGGAGTTCCAGAAGCTTGTCCAGCTGCTGCTATCTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005560
• Insert Size: 2424 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005560.1, NP_001005560.1
• RefSeq Size: 3100 bp
• RefSeq ORF: 2424 bp
• Locus ID: 360426
• UniProt ID: P97570
• Cytogenetics: 7q34
• Gene Summary: has Ca2+ independent phospholipase A2 activity; may play a role in regulating transmembrane ionic fluxes in pancreatic beta cells [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).