Agt (NM_134432) Rat Tagged ORF Clone

CAT#: RR206780

Agt (Myc-DDK-tagged ORF) - Rat angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (Agt), (10 ug)

Product Images

Circular map for RR206780

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agt
• Synonyms: Ang; AngII; ANRT; PAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206780 representing NM_134432
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCCACGGGGGCAGGCCTGAAGGCCACCATCTTCTGCATCCTGACCTGGGTCAGCCTGACAGCTG
  GGGACCGCGTATACATCCACCCCTTTCATCTCCTCTACTACAGCAAGAGCACCTGCGCCCAGCTGGAGAA
  CCCCAGTGTGGAGACGCTCCCAGAGCCAACCTTTGAGCCTGTGCCCATTCAGGCCAAGACCTCCCCCGTG
  GATGAGAAGACCCTGCGAGATAAGCTCGTGCTGGCCACTGAGAAGCTAGAGGCTGAGGATCGGCAGCGAG
  CTGCCCAGGTCGCGATGATTGCCAACTTCATGGGTTTCCGCATGTACAAGATGCTGAGTGAGGCAAGAGG
  TGTAGCCAGTGGGGCCGTCCTCTCTCCACCGGCCCTCTTTGGCACCCTGGTCTCTTTCTACCTTGGATCG
  TTGGATCCCACGGCCAGCCAGTTGCAGGTGCTGCTGGGCGTCCCTGTGAAGGAGGGAGACTGCACCTCCC
  GGCTGGACGGACATAAGGTCCTCACTGCCCTGCAGGCTGTTCAGGGCTTGCTGGTCACCCAGGGTGGAAG
  CAGCAGCCAGACACCCCTGCTACAGTCCACCGTGGTGGGCCTCTTCACTGCCCCAGGCTTGCGCCTAAAA
  CAGCCATTTGTTGAGAGCTTGGGTCCCTTCACCCCCGCCATCTTCCCTCGCTCTCTGGACTTATCCACTG
  ACCCAGTTCTTGCTGCCCAGAAAATCAACAGGTTTGTGCAGGCTGTGACAGGGTGGAAGATGAACTTGCC
  ACTAGAGGGGGTCAGCACGGACAGCACCCTATTTTTCAACACCTACGTTCACTTCCAAGGGAAGATGAGA
  GGCTTCTCCCAGCTGACTGGGCTCCATGAGTTCTGGGTGGACAACAGCACCTCAGTGTCTGTGCCCATGC
  TCTCGGGCACTGGCAACTTCCAGCACTGGAGTGACGCCCAGAACAACTTCTCCGTGACACGCGTGCCCCT
  GGGTGAGAGTGTCACCCTGCTGCTGATCCAGCCCCAGTGCGCCTCAGATCTCGACAGGGTGGAGGTCCTC
  GTCTTCCAGCACGACTTCCTGACTTGGATAAAGAACCCGCCTCCTCGGGCCATCCGTCTGACCCTGCCGC
  AGCTGGAAATTCGGGGATCCTACAACCTGCAGGACCTGCTGGCTCAGGCCAAGCTGTCTACCCTTTTGGG
  TGCTGAGGCAAATCTGGGCAAGATGGGTGACACCAACCCCCGAGTGGGAGAGGTTCTCAACAGCATCCTC
  CTTGAACTCCAAGCAGGCGAGGAGGAGCAGCCCACAGAGTCTGCCCAGCAGCCTGGCTCACCCGAGGTGC
  TGGACGTGACCCTGAGCAGTCCGTTCCTGTTCGCCATCTACGAGCGGGACTCAGGTGCGCTGCACTTTCT
  GGGCAGAGTGGATAACCCCCAAAATGTGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206780 representing NM_134432
  Red=Cloning site Green=Tags(s)
  
  MTPTGAGLKATIFCILTWVSLTAGDRVYIHPFHLLYYSKSTCAQLENPSVETLPEPTFEPVPIQAKTSPV
  DEKTLRDKLVLATEKLEAEDRQRAAQVAMIANFMGFRMYKMLSEARGVASGAVLSPPALFGTLVSFYLGS
  LDPTASQLQVLLGVPVKEGDCTSRLDGHKVLTALQAVQGLLVTQGGSSSQTPLLQSTVVGLFTAPGLRLK
  QPFVESLGPFTPAIFPRSLDLSTDPVLAAQKINRFVQAVTGWKMNLPLEGVSTDSTLFFNTYVHFQGKMR
  GFSQLTGLHEFWVDNSTSVSVPMLSGTGNFQHWSDAQNNFSVTRVPLGESVTLLLIQPQCASDLDRVEVL
  VFQHDFLTWIKNPPPRAIRLTLPQLEIRGSYNLQDLLAQAKLSTLLGAEANLGKMGDTNPRVGEVLNSIL
  LELQAGEEEQPTESAQQPGSPEVLDVTLSSPFLFAIYERDSGALHFLGRVDNPQNVV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134432
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134432.2, NP_602308.1
• RefSeq Size: 1958 bp
• RefSeq ORF: 1434 bp
• Locus ID: 24179
• UniProt ID: P01015
• Cytogenetics: 19q12
• MW: 52 kDa
• Gene Summary: plays a role in regulation of blood pressure [RGD, Feb 2006]