Irf6 (NM_001108859) Rat Tagged ORF Clone

CAT#: RR206734

Irf6 (Myc-DDK-tagged ORF) - Rat interferon regulatory factor 6 (Irf6), (10 ug)

Product Images

Circular map for RR206734

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Irf6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206734 representing NM_001108859
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCCACCCTCGAAGAGTCCGGCTGAAGCCCTGGCTGGTGGCCCAGGTGGACAGTGGCCTCTACC
  CTGGTCTCATCTGGCTACATAGAGATTCCAAACGCTTCCAGATCCCCTGGAAACATGCCACGCGGCACAG
  CCCCCAACAAGAGGAAGAAAACACCATTTTTAAGGCTTGGGCTGTGGAGACTGGAAAGTACCAGGAAGGG
  GTGGACGATCCTGACCCAGCTAAATGGAAGGCTCAGCTCCGATGTGCTCTCAACAAAAGCCGGGAATTCA
  ACTTGATGTATGACGGCACCAAGGAAGTGCCCATGAATCCTGTGAAGATCTATCAAGTGTGTGACATCCC
  CCAGCCCCAGGGCTCTGTCATTAACCCAGGATCCACAGGGTCTGCTCCTTGGGATGAGAAAGATAATGAT
  GTGGATGAAGATGACGAGGAAGATGAACTTGAACAGTCACAGCACCACGTCCCGATCCAGGACACCTTCC
  CCTTCCTGAACATCAATGGTTCTCCCATGGCACCAGCCAGCGTGGGCAACTGCAGTGTGGGAAACTGCAG
  CCCTGAATCAGTGTGGCCCAAAACAGAACCTCTGGAAATGGAAGTACCGCAGGCACCCATTCAGCCCTTC
  TATAGTTCTCCAGAGCTGTGGATCAGCTCCCTCCCCATGACTGACTTGGACATCAAGTTTCAGTATCGTG
  GGAAGGAGTATGGGCAAACCATGACGGTGAGCAACCCCCAGGGCTGCCGGCTCTTCTATGGGGACCTGGG
  CCCCATGCCTGACCAGGAGGAGCTCTTTGGTCCTGTCAGCCTGGAGCAGGTCAAGTTCCCGGGGCCAGAG
  CATATCACCAATGAGAAGCAGAAGCTGTTCACCAGTAAGCTGTTGGATGTCATGGACAGGGGACTGATCC
  TGGAGGTCAGCGGACATGCCATTTATGCCATCCGGCTGTGCCAGTGCAAAGTGTACTGGTCGGGGCCGTG
  TGCCCCCTCACTTGCTGCTCCTAACCTGATTGAGAGGCAGAAGAAGGTCAAGTTGTTTTGTCTGGAAACA
  TTCCTGAGTGAGCTCATTGCCCATCAGAAAGGGCAGATAGAGAAGCAGCCACCTTTCGAGATTTACTTAT
  GCTTCGGGGAAGAATGGCCAGATGGAAAGCCCCTGGAGAGGAAGCTCATCTTGGTCCAGGTCATTCCAGT
  GGTGGCTCGCATGATCTATGAGATGTTTTCTGGTGATTTCACCCGGTCCTTTGACAGTGGCAGTGTTCGC
  CTGCAGATTTCCACTCCAGACATCAAAGATAACATCGTCGCTCAGCTCAAGCAGCTGTACCGCATCCTTC
  AAACCCAGGAGAGCTGGCAGCCCATGCAGCCTGCCCCCAGCATGCAGCTGCCGCCTGCCCTGCCTGCCCA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206734 representing NM_001108859
  Red=Cloning site Green=Tags(s)
  
  MALHPRRVRLKPWLVAQVDSGLYPGLIWLHRDSKRFQIPWKHATRHSPQQEEENTIFKAWAVETGKYQEG
  VDDPDPAKWKAQLRCALNKSREFNLMYDGTKEVPMNPVKIYQVCDIPQPQGSVINPGSTGSAPWDEKDND
  VDEDDEEDELEQSQHHVPIQDTFPFLNINGSPMAPASVGNCSVGNCSPESVWPKTEPLEMEVPQAPIQPF
  YSSPELWISSLPMTDLDIKFQYRGKEYGQTMTVSNPQGCRLFYGDLGPMPDQEELFGPVSLEQVKFPGPE
  HITNEKQKLFTSKLLDVMDRGLILEVSGHAIYAIRLCQCKVYWSGPCAPSLAAPNLIERQKKVKLFCLET
  FLSELIAHQKGQIEKQPPFEIYLCFGEEWPDGKPLERKLILVQVIPVVARMIYEMFSGDFTRSFDSGSVR
  LQISTPDIKDNIVAQLKQLYRILQTQESWQPMQPAPSMQLPPALPAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108859
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108859.1, NP_001102329.1
• RefSeq Size: 4044 bp
• RefSeq ORF: 1404 bp
• Locus ID: 364081
• Cytogenetics: 13q27
• MW: 53.1 kDa